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中华临床医师杂志(电子版) ›› 2017, Vol. 11 ›› Issue (16) : 2109 -2114. doi: 10.3877/cma.j.issn.1674-0785.2017.16.001

所属专题: 文献

临床论著

云南西双版纳地区乙型肝炎病毒基因型和基因亚型研究
高建梅1,(), 张雯1, 左荣霞1, 王金丽1, 严新民1   
  1. 1. 650032 昆明,云南省第一人民医院临床基础医学研究所
  • 收稿日期:2017-03-08 出版日期:2017-08-15
  • 通信作者: 高建梅
  • 基金资助:
    云南省应用基础研究计划—昆医联合专项(2014FB094); 云南省卫生科技计划项目(2017NS225)

Hepatitis B virus genotypes and subgenotypes in Xishuangbanna, China

Jianmei Gao1,(), Wen Zhang1, Rongxia Zuo1, Jinli Wang1, Xinming Yan1   

  1. 1. Institute of Clinical and Basic Medicine Research, the First People’s Hospital of Yunnan Province, Kunming 650032, China
  • Received:2017-03-08 Published:2017-08-15
  • Corresponding author: Jianmei Gao
  • About author:
    Corresponding author: Gao Jianmei, Email:
引用本文:

高建梅, 张雯, 左荣霞, 王金丽, 严新民. 云南西双版纳地区乙型肝炎病毒基因型和基因亚型研究[J/OL]. 中华临床医师杂志(电子版), 2017, 11(16): 2109-2114.

Jianmei Gao, Wen Zhang, Rongxia Zuo, Jinli Wang, Xinming Yan. Hepatitis B virus genotypes and subgenotypes in Xishuangbanna, China[J/OL]. Chinese Journal of Clinicians(Electronic Edition), 2017, 11(16): 2109-2114.

目的

探讨云南西双版纳地区乙型肝炎病毒(HBV)基因型和基因亚型分布情况。

方法

选取2012年5月至2013年4月西双版纳地区汉族和人数最多的两个少数民族(傣族和哈尼族)人群为研究对象,采用巢式PCR、基因序列测定等技术获取HBV全基因组序列后,构建系统进化树并计算遗传距离鉴定HBV基因型和基因亚型。

结果

在此地区人群中共检出3种HBV基因型:B基因型139例(76.0%),其中以B2亚型为主(105例,57.4%),quasi B3亚型次之(34例,18.6%);C基因型41例(22.4%),主要为C1亚型(33例,18.0%),其次为C2(4例,2.2%)和C5(4例,2.2%)亚型;I基因型3例(1.6%)。三民族人群HBV基因型与基因亚型分布比较:哈尼族人群HBV基因型分布与其他两个民族显著不同(哈尼族vs汉族:χ2=8.416,P=0.006;哈尼族vs傣族:χ2=15.636,P<0.001),其B基因型显著高于其他两个民族,同时没有发现I基因型;三民族人群HBV基因亚型组成也显著不同(汉族vs傣族:χ2=33.598,P<0.001;汉族vs哈尼族:χ2=22.102,P<0.001;哈尼族vs傣族:χ2=16.129,P=0.001),quasi B3亚型仅存在于傣族和哈尼族等少数民族人群,汉族人群中未检出;傣族C1亚型较其他两个民族人群高,且C5仅在傣族人群中发现;哈尼族人群C基因亚型全部为C1亚型。

结论

此地区HBV基因型和基因亚型分布复杂且不同民族人群HBV基因型和基因亚型分布具有民族的自身特点。

Objective

To investigate the distribution of hepatitis B virus (HBV) genotypes and subgenotypes in Xishuangbanna, Yunnan Province, China.

Methods

Study subjects were selected from people of Han nationality and two ethnic minorities (Dai and Hani) from May 2012 to April 2013, which are the two largest ethnic groups in Xishuangbanna. Nest-PCR and gene sequencing were performed to obtain the full genome sequence of HBV, and HBV genotypes and subgenotypes were analyzed by phylogenetic tree construction and genetic distance calculation.

Results

Tree analysis of the full genome sequence of HBV detected three HBV genotypes in this areas: 139 (76.0%) cases of genotype B, among which 105 (57.4%) were subgenotype B2 and 34 (18.6%) were subgenotype quasi B3; 41 (22.4%) cases of genotype C, among which 33 (18.0%) were subgenotype C1 (18.0%), 4 (2.2%) were subgenotype C2, and 4 (2.2%) were subgenotype C5 (2.2%); and 3 (1.6%) cases of genotype I. Comparative analysis of HBV genotype and subgenotype distribution in the three populations revealed that the HBV genotype distribution in Hani people was significantly different from that in other two ethnic groups (Hani vs Han: χ2 = 8.416, P = 0.006; Hani vs Dai: χ2 = 15.636, P<0.001); the prevalence of genotype B was significantly higher in Hani people than in the other two groups, but genotype I was not detected in Hani people. The distribution of HBV subgenotypes in the three populations was significantly different as well (Han vs Dai: χ2 = 33.598, P< 0.001; Han vs Hani: χ2 = 22.102, P< 0.001; Hani vs Dai: χ2 = 16.129, P = 0.001). Subgenotype quasi B3 existed only in Dai and Hani minority populations but not in Han nationality. The prevalence of subgenotype C1 in Dai people was higher than that in the other two ethnic groups, and subgenotype C5 was detected only in Dai people. The subgenotypes of genotype C in Hani population were all subgenotype C1.

Conclusion

The distribution of HBV genotypes and subgenotypes in this area is complicated and has unique characteristics among different ethnic groups.

表1 研究样本一般人口学资料
表2 HBV-DNA PCR扩增及测序引物
表3 西双版纳地区景洪市汉族、傣族和哈尼族人群基因型分布比较[例(%)]
图1 HBV全基因序列系统进化树分析
表4 西双版纳地区景洪市汉族、傣族和哈尼族人群基因亚型分布比较[例(%)]
图2 HBV B基因亚型系统进化树分析
图3 HBV C基因亚型系统进化树分析
图4 HBV I基因亚型系统进化树分析
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