血清及脑脊液中白介素-1β、白介素-6及肿瘤坏死因子-α水平与病毒性脑炎急性期伴癫痫发作的关系

doi:10.3877/cma.j.issn.1674-0785.2017.16.003

目的

探讨病毒性脑炎急性期伴癫痫发作与血清及脑脊液白介素-1β（IL-1β）、白介素-6（IL-6）及肿瘤坏死因子-α（TNF-α）水平之间的关系。

方法

收集2016年3月至2017年1月于山西医科大学第一临床医院神经内科住院的30例病毒性脑炎急性期患者，根据有无癫痫发作，分为病毒性脑炎急性期伴癫痫发作组（10例）和病毒性脑炎急性期不伴癫痫发作组（20例）。同时选取10例非病毒性脑炎性癫痫患者和10例需脊髓麻醉并排除感染及炎症的患者作为对照组。采集各组患者的血液和脑脊液，并采用夹心法酶联免疫吸附试验（ELISA）方法测定各组患者血清和脑脊液IL-1β、IL-6及TNF-α浓度水平，比较各组差异。

结果

（1）病毒性脑炎患者急性期血清及脑脊液IL-1β、IL-6及TNF-α浓度水平明显高于对照组（P<0.05）；（2）病毒性脑炎急性期伴癫痫发作组较病毒性脑炎急性期不伴癫痫发作组血清及脑脊液IL-1β、IL-6及TNF-α水平明显升高（P<0.05）。

结论

病毒性脑炎急性期伴癫痫发作血清及脑脊液IL-1β、IL-6及TNF-α水平明显高于病毒性脑炎急性期不伴癫痫发作患者。

关键词: 病毒性脑炎, 急性期, 癫痫发作, 细胞因子

Objective

To investigate the relationship between interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) levels in serum and cerebrospinal fluid and acute viral encephalitis with seizures.

Methods

Thirty patients with viral encephalitis were enrolled in the Department of Neurology, the First Clinical Hospital of Shanxi Medical University from March 2016 to January 2017. According to the presence or absence of seizures, the patients were divided into two groups: patients with seizures and those without seizures. At the same time, ten non-viral encephalitis patients with seizures and ten patients undergoing spinal cord anesthesia without infection or inflammation were included as controls. Blood and cerebrospinal fluid were collected from each group. The levels of IL-1β, IL-6, and TNF-α in serum and cerebrospinal fluid were measured by enzyme-linked immunosorbent assay.

Results

The levels of IL-1β, IL-6, and TNF-α in serum and cerebrospinal fluid were significantly higher in patients with viral encephalitis than in controls (P<0.05), and in acute viral encephalitis patients with seizures than in those without (P<0.05).

Conclusion

The levels of IL-1β, IL-6, and TNF-α in serum and cerebrospinal fluid are significantly higher in acute viral encephalitis patients with seizures than in those without.

Key words: Viral encephalitis, Epilepsy, Seizure, Cytokines

表1 各组血清IL-1β、IL-6及TNF-α水平比较（ng/ml，±s）

组别	例数	IL-1β	IL-6	TNF-α
A组	20	2.474±0.221	21.318±2.542	29.585±3.861
B组	10	2.675±0.072^a	22.878±1.232^a	25.789±6.378^a
C组	10	1.144±0.020^ab	6.491±0.545^ab	20.023±3.407^ab
D组	10	1.049±0.033^ab	6.669±0.294^ab	20.902±5.930^ab

表1 各组血清IL-1β、IL-6及TNF-α水平比较（ng/ml，±s）

组别	例数	IL-1β	IL-6	TNF-α
A组	20	2.474±0.221	21.318±2.542	29.585±3.861
B组	10	2.675±0.072^a	22.878±1.232^a	25.789±6.378^a
C组	10	1.144±0.020^ab	6.491±0.545^ab	20.023±3.407^ab
D组	10	1.049±0.033^ab	6.669±0.294^ab	20.902±5.930^ab

表2 各组脑脊液IL-1β、IL-6及TNF-α水平比较（ng/ml，±s）

组别	例数	IL-1β	IL-6	TNF-α
A组	20	3.073±0.565	631.972±58.733	33.227±3.016
B组	10	3.573±0.148^a	950.794±1.926^a	28.958±3.676^a
C组	10	1.269±0.332^ab	108.763±1.536^ab	24.269±1.700^ab
D组	10	1.318±0.243^ab	103.463±0.481^ab	25.043±3.708^ab

表2 各组脑脊液IL-1β、IL-6及TNF-α水平比较（ng/ml，±s）

组别	例数	IL-1β	IL-6	TNF-α
A组	20	3.073±0.565	631.972±58.733	33.227±3.016
B组	10	3.573±0.148^a	950.794±1.926^a	28.958±3.676^a
C组	10	1.269±0.332^ab	108.763±1.536^ab	24.269±1.700^ab
D组	10	1.318±0.243^ab	103.463±0.481^ab	25.043±3.708^ab

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Relationship between interleukin-1β, interleukin-6, and tumor necrosis factor-α levels in serum and cerebrospinal fluid and acute viral encephalitis with seizures

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Relationship between interleukin-1β, interleukin-6, and tumor necrosis factor-α levels in serum and cerebrospinal fluid and acute viral encephalitis with seizures