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中华临床医师杂志(电子版) ›› 2018, Vol. 12 ›› Issue (05) : 268 -272. doi: 10.3877/cma.j.issn.1674-0785.2018.05.003

所属专题: 文献

临床研究

HBV基因型、基因亚型及基因变异与原发性肝癌的关系
石光英1, 郭新文2, 谢敬东3,()   
  1. 1. 830002 乌鲁木齐,新疆生产建设兵团医院消化肝病科
    2. 843000 新疆维吾尔自治区阿克苏地区第一人民医院消化科
    3. 200025 上海交通大学医学院附属瑞金医院感染科
  • 收稿日期:2017-12-29 出版日期:2018-03-01
  • 通信作者: 谢敬东
  • 基金资助:
    新疆兵团科技计划项目(2013AB028)院级课题(2016AD011)

Correlation of hepatocellular carcinoma with hepatitis B virus genotype, subgenotype, and mutation

Guangying Shi1, Xinwen Guo2, Jingdong Xie3,()   

  1. 1. Department of Hepatology, Xinjiang Production and Construction Corps Hospital, Urumqi 830002, China
    2. Department of Digestive Medicine, Aksu Region First People's Hospital of Xinjiang Uygur Autonomous Region, Aksu 843000, China
    3. Department of Infection, Ruijin Hospital, Shanghai Jiaotong University Medical College, Shanghai 200025, China
  • Received:2017-12-29 Published:2018-03-01
  • Corresponding author: Jingdong Xie
  • About author:
    Corresponding author: Xie Jingdong, Email:
引用本文:

石光英, 郭新文, 谢敬东. HBV基因型、基因亚型及基因变异与原发性肝癌的关系[J/OL]. 中华临床医师杂志(电子版), 2018, 12(05): 268-272.

Guangying Shi, Xinwen Guo, Jingdong Xie. Correlation of hepatocellular carcinoma with hepatitis B virus genotype, subgenotype, and mutation[J/OL]. Chinese Journal of Clinicians(Electronic Edition), 2018, 12(05): 268-272.

目的

探讨乙型肝炎病毒(HBV)不同基因型、基因亚型及基因变异与原发性肝癌(PHC)的关系,为制定PHC的预防和治疗措施提供科学依据。

方法

收集新疆生产建设兵团医院2013年3月至2016年3月共4例感染HBV基础上的PHC患者,利用荧光定量PCR法检测HBV DNA,酶联免疫法检测HBeAg。应用PCR方法检测患者HBV基因型、基因亚型分布特征,检测HBV基因变异情况。

结果

此4例感染HBV基础上的PHC患者中,3例患者HBeAg(+),HBeAg阳性率为75%。4例患者中3例HBV DNA(+),定量检测结果分别为2.03×105 IU/ml、2.56×106 IU/ml、3.12×105 IU/ml;1例患者HBV DNA(-)。检测患者基因型为B型1例(B2亚型),C型3例(C1亚型1例,C2亚型2例)。HBV B2亚型、C1亚型和1例C2亚型患者,分别在APC、CTNNB1、IGF2R基因处出现基因变异;1例C2亚型患者(80岁),在UGT1A5、ZFYVE16、MALRD1基因的外显子序列检测中发现移码变异。

结论

HBV基因型,尤其是C型可能是PHC发生的危险因素。基因变异与PHC的发生发展可能也存在密切关系。

Objective

To analyze the correlation of hepatocellular carcinoma with hepatitis B virus genotype, subgenotype, or mutation, so as to provide a scientific basis for the prevention of hepatocellular carcinoma.

Methods

Totally four patients with HBV associated primary hepatocellular carcinoma (PHC) were collected in this study. HBV DNA was tested by qRT-PCR. HBeAg was tested by enzyme linked immunosorbent assay. HBV genotypes were determined by PCR-restriction fragment length polymorphism. HBV subgenotypes were determined by nested PCR. HBV DNA mutations were determined by PCR or direct sequencing.

Results

Among the four patients with PHC, the positive rate of HBeAg was 75%. HBV DNA quantitative detection was positive in three patients (2.03×105 IU/mL, 2.56×106 IU/mL, and 3.12×105 IU/mL), and one case was HBV DNA negative. The detected genotypes included type B in one case (subtype B2) and type C in three cases, among which one was subtype C1 and two were subtype C2. Thus, in PHC patients, the main genotype and subgenotype were C and C2, respectively.

Conclusion

HBV genotypes, especially genotype C and subgenotype C2 variants, may be risk factors for the development of PHC. The gene mutations of HBV DNA may be closely related to the occurrence and development of PHC.

表1 3例原发性肝癌患者基因变异情况
表2 1例80岁原发性肝癌患者(乙型肝炎病毒C2亚型)基因变异情况
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