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中华临床医师杂志(电子版)

中华临床医师杂志(电子版) ›› 2019, Vol. 13 ›› Issue (07) : 498 -503. doi: 10.3877/cma.j.issn.1674-0785.2019.07.004

所属专题： 文献；

临床研究

基于数据挖掘分析GPX4在胃癌中的表达及临床意义
王首寒1, 王斌1, 陈佳祺1, 程显斌1, 孙小单2,†()   
  1. 1. 130012　长春，吉林省肿瘤医院肝胆脾外科
    2. 130012　长春，吉林省肿瘤医院妇瘤二科
  • 收稿日期:2019-02-16 出版日期:2019-04-01
  • 通信作者: 孙小单

Clinical significance of expression of GPX4 in gastric cancer

Shouhan Wang1, Bin Wang1, Jiaqi Chen1, Xianbin Cheng1, Xiaodan Sun2,†()   

  1. 1. Department of Hepatopancreatobiliary Surgery, Jilin Province Cancer Hospital, Changchun 130012, China
    2. Department of Gynecologic Oncology (Division II), Jilin Province Cancer Hospital, Changchun 130012, China
  • Received:2019-02-16 Published:2019-04-01
  • Corresponding author: Xiaodan Sun
  • About author:
    Corresponding author: Sun Xiaodan, Email:
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引用本文：

王首寒, 王斌, 陈佳祺, 程显斌, 孙小单. 基于数据挖掘分析GPX4在胃癌中的表达及临床意义[J]. 中华临床医师杂志(电子版), 2019, 13(07): 498-503.

Shouhan Wang, Bin Wang, Jiaqi Chen, Xianbin Cheng, Xiaodan Sun. Clinical significance of expression of GPX4 in gastric cancer[J]. Chinese Journal of Clinicians(Electronic Edition), 2019, 13(07): 498-503.

目的

探索谷胱甘肽过氧化物酶4（GPX4）在胃癌中的表达及临床意义。

方法

检索收集Oncomine和基因表达谱动态分析（GEPIA）数据库中GPX4 mRNA在常见肿瘤中的表达情况，并分析其在胃癌中的表达及与患者预后的关系。利用cBioPortal在线分析癌症基因图谱（TCGA）数据库中1178例胃癌样本中的RNA测序数据，分析GPX4基因的突变情况及其与预后的关系。利用String数据库预测与GPX4相互作用的蛋白。

结果

Oncomine数据库中共收集了459项关于GPX4基因在常见肿瘤及正常组织中的对照研究，其中22项表达差异具有统计学意义（P<0.0001）。GEPIA数据库分析结果显示GPX4 mRNA在胃癌组织中高表达（P<0.01），且与不良的总体生存期及无进展生存期明显相关（P<0.05），但与胃癌的TNM分期无明显相关性（P>0.05）。对TCGA数据库的RNA测序结果分析发现，在1178例胃癌样本中有25例发生GPX4基因变异，总变异率为2.1%。Kaplan-Meier生存曲线分析显示，GPX4基因变异与否，与总体生存期及无进展生存期无明显相关性（P>0.05）。GSR、GSS、GGT1/5/6/7，GSTO2，GRSF1，SOD1/2等蛋白与GPX4有明显的相互作用（P=1.94×10-7）。

结论

利用多种肿瘤基因数据库分析表明，GPX4基因在胃癌组织中变异率低，其mRNA水平高表达与患者不良预后相关，为进一步探究GPX4在胃癌的发生发展中的作用提供了重要理论依据。

关键词: 谷胱甘肽过氧化物酶, 胃肿瘤, 数据库
Objective

To investigate the expression of glutathione peroxidase 4 (GPX4) in gastric cancer and to analyze its clinical significance.

Methods

The expression of GPX4 mRNA in common tumors was analyzed based on Oncomine and Gene Expression Profiling Interactive Analysis (GEPIA) databases, and its expression in gastric cancer tissues and relationship with prognosis were also analyzed. The RNA sequencing data of 1178 gastric cancer samples in The Cancer Geneome Atlas (TCGA) database were obtained via cBioPortal to analyze the mutations of the GPX4 gene and their relationship with prognosis. Proteins interacting with GPX4 were predicted using the String database.

Results

A total of 459 studies on GPX4 gene in common tumors and normal tissues were collected from the Oncomine database, 22 of which showed significantly differential GPX4 expression (P<0.0001). GEPIA database analysis showed that GPX4 mRNA was highly expressed in gastric cancer tissues (P<0.01), which was significantly associated with poor overall survival and progression-free survival (P<0.05), but had no significant correlation with TNM stage of gastric cancer (P>0.05). Analysis of RNA sequencing results in the TCGA database revealed that GPX4 gene mutations occurred in 25 of 1178 gastric cancer samples, with a total mutation rate of 2.1%. Kaplan-Meier survival curve analysis showed that there was no significant correlation between GPX4 gene mutation and OS or PFS (P>0.05). Proteins such as GSR, GSS, GGT1/5/6/7, GSTO2, GRSF1, and SOD1/2 had obvious interactions with GPX4 (P=1.94×10-7).

Conclusion

The analysis of multiple tumor gene databases shows that the GPX4 gene has a low mutation rate in gastric cancer tissues, and its high mRNA expression is associated with a poor prognosis. Our findings provide an important theoretical basis for further exploration of the role for GPX4 in the development of gastric cancer.

Key words: Glutathione peroxidase, Gastric cancer, Database
图1 Oncomine数据库中GPX4在常见肿瘤类型中的表达
图2 GEPIA数据库中GPX4在胃癌及正常胃组织中的表达差异
图3 GEPIA数据库中GPX4在胃癌不同临床分期中的表达
图4 GEPIA数据库中GPX4表达与胃癌患者预后的关系　图a为总生存率的生存曲线，图b为无病生存率的生存曲线
图5 TCGA数据库中GPX4基因在胃癌中的变异情况
图6 GPX4基因变异与患者预后的关系　图a为总生存率生存曲线，图b为无病生存率生存曲线
图7 与GPX4基因表达蛋白相关的蛋白网络图
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