富氢生理盐水对新生SD大鼠高氧肺损伤白介素-8含量及病理的影响

doi:10.3877/cma.j.issn.1674-0785.2019.10.011

中华临床医师杂志(电子版) ›› 2019, Vol. 13 ›› Issue (10) : 767 -770. doi: 10.3877/cma.j.issn.1674-0785.2019.10.011

所属专题：文献；

基础研究

富氢生理盐水对新生SD大鼠高氧肺损伤白介素-8含量及病理的影响

吴春友¹^,^†(

), 宋玉环¹, 苏永红¹

^1. 262700　潍坊市妇幼保健院综合儿科

收稿日期:2019-03-24 出版日期:2019-05-15
通信作者: 吴春友
基金资助:
潍坊市科技局资助项目(2017YX073)

Effects of hydrogen-rich saline on interleukin-8 and pathology in rats with hyperoxia-induced lung injury

Chunyou Wu¹^,^†(), Yuhuan Song¹, Yonghong Su¹

^1. Department of Comprehensive Pediatrics, Weifang Maternal and Child Health Hospital, Weifang 262700, China

Received:2019-03-24 Published:2019-05-15
Corresponding author: Chunyou Wu
About author:
Corresponding author: Wu Chunyou, Email: 13626460831@163.com

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目的

观察富氢生理盐水对新生SD大鼠高氧肺损伤的预防作用。

方法

将新生SD大鼠随机分为3组，空气对照组、高氧对照组、高氧＋氢水组。高氧对照组、高氧＋氢水组大鼠置于氧浓度为90%~95%的氧气箱中，高氧＋氢水组每日腹腔注射富氢生理盐水2次（10 ml/kg），空气对照组、高氧对照组每日腹腔注射生理盐水2次（10 ml/kg），高氧暴露后第1、3、7、10天分别测量各组白介素（IL）-8水平，并观察肺部病理切片。

结果

与空气对照组对比，高氧对照组IL-8含量明显升高，且肺部病理切片出现肺出血、肺泡间隔变薄，并出现大量肺泡融合现象；与高氧对照组比较，高氧+氢水组IL-8含量明显降低，病理图示肺出血、肺泡融合明显减少。

结论

富氢生理盐水可一定程度减轻高氧对新生SD大鼠的肺部炎症损伤。

关键词: 富氢生理盐水, 高氧肺损伤, 白介素-8

Objective

To observe the effect of hydrogen-rich saline on hyperoxia-induced lung injury in neonatal Sprague-Dawley (SD) rats.

Methods

Newborn SD rats were randomly divided into three groups: air group, hyperoxia group, and hyperoxia + hydrogen-rich saline group. The hyperoxia group and the hyperoxia+ hydrogen-rich saline group were placed in an oxygen chamber with a 90%-95% oxygen concentration. The hyperoxia + hydrogen-rich saline group was injected with hydrogen-rich saline twice a day (10 ml/kg). The air group and hyperoxia group were injected with normal saline twice a day (10 ml/kg). The clinical manifestations of each group were recorded at 1, 3, 7, and 10 days after birth. At the same time, the alveolar lavage fluid of each group was taken and interleukin-8 was detected by enzyme linked immunosorbent assay (ELISA), and the pathological changes of the lungs were observed.

Results

Compared with the air group, the content of interleukin-8 in the hyperoxia group was significantly higher. In the hyperoxia group, pulmonary hemorrhage, thinning of alveolar septum, and massive alveolar fusion were observed. Compared with the hyperoxia group, the content of interleukin-8 in the hyperoxia+ hydrogen-rich saline group was significantly lower, and pulmonary hemorrhage and pulmonary alveolar fusion were significantly reduced.

Conclusion

Hydrogen-rich saline can alleviate hyperoxia-induced lung inflammation in neonatal SD rats.

Key words: Hydrogen-rich saline, Hyperoxia-induced lung injury, Interleukin-8

表1 3组新生鼠不同高浓度氧暴露时间肺组织白介素-8水平的比较（±s）

分组	只数	1 d	3 d	7 d	10 d
空气对照组	16	92.15±28.71	67.78±32.23^a	59.38±36.13^a	77.94±38.43^a
高氧对照组	16	128.27±65.49	274.65±89.72	353.81±46.51	317.57±86.05
高氧＋氢水组	16	110.54±22.65	154.85±71.53^a	263.36±27.87^a	197.59±61.31^a
F值	?	0.52	6.84	48.22	10.23
P值	?	0.6181	0.0284	0.0002	0.0117

表1 3组新生鼠不同高浓度氧暴露时间肺组织白介素-8水平的比较（±s）

分组	只数	1 d	3 d	7 d	10 d
空气对照组	16	92.15±28.71	67.78±32.23^a	59.38±36.13^a	77.94±38.43^a
高氧对照组	16	128.27±65.49	274.65±89.72	353.81±46.51	317.57±86.05
高氧＋氢水组	16	110.54±22.65	154.85±71.53^a	263.36±27.87^a	197.59±61.31^a
F值	?	0.52	6.84	48.22	10.23
P值	?	0.6181	0.0284	0.0002	0.0117

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