探讨CD147表达对结肠癌细胞增殖、凋亡的影响和潜在的分子机制。

分析UCSC数据库中基因BSG（编码CD147蛋白）在结肠癌中的表达，利用Kaplan-Meier Plotter数据库评估BSG的表达与生存结局之间的关系。应用Western blot检测CD147在HCT116和HCoEPiC细胞中的表达，通过慢病毒转染HCT116细胞下调CD147表达进一步验证其转染效率。应用CCK-8、平板克隆和Hoechst 33342染色实验检测细胞增殖和凋亡能力。Western blot检测各组细胞内MAPK、p-MAPK、C-MYC、BAX和BCL-2的表达；同时应用TIMER 2.0数据库对结肠癌组织中的BSG表达和MAPK1、MYC、BAX、BCL-2进行相关性分析。

UCSC数据库观察到CD147在结肠癌组织中表达高于正常组织（P<0.01）；过表达CD147患者的预后不良。Western blot结果显示，与HCoEPiC细胞比较，HCT116细胞中CD147蛋白表达增高（P<0.001）。荧光显微镜下shCD147低表达组与sh-NON空载组细胞的荧光表达丰度均可达90%；Western blot结果显示，与HCT116细胞比较，shCD147组细胞中CD147蛋白表达降低（P<0.001）。下调CD147后，与HCT116组比较，sh-CD147组细胞增殖和细胞集落形成能力降低；Hoechst 33342染色结果显示，与HCT116组相比，shCD147组凋亡的细胞核染色增强，荧光更为明亮（P<0.001）。与HCT116相比，sh-CD147组细胞内p-MAPK、C-MYC和BCL-2表达降低，但BAX表达升高；TIMER 2.0数据库相关性分析，结肠癌组织中BSG表达水平与MAPK1、MYC、BCL-2表达呈现正相关，但与BAX表达呈现负相关（P<0.001）。

下调CD147表达降低结肠癌细胞增殖能力，促进凋亡发生，该过程可能与抑制MAPK信号通路有关。

To investigate the effect of CD147 expression on the proliferation and apoptosis of colon cancer cells and the potential underlying molecular mechanism.

The expression level of BSG gene (encoding CD147 protein) in colon cancer was analyzed in the UCSC database, and the relationship between BSG expression and survival outcome was evaluated based on the Kaplan-Meier Plotter database. The expression of CD147 in HCT116 and HCoEPiC cells was detected by Western blot. Then, lentivirus-mediated shRNA transfection was used to down-regulate the expression of CD147 in HCT116 cells using shRNA against CD147 (shCD147), with the empty vector (sh-NON) used as the negative control. CCK-8 assay, plate clone formation assay, and Hoechst 33342 staining were used to detect cell proliferation and apoptosis. The expression of MAPK, p-MAPK, C-MYC, BAX, and BCL-2 was detected by Western blot. The correlation between the expression of BSG and MAPK1, MYC, BAX, and BCL-2 in colon cancer was analyzed based on the TIMER2.0 database.

The expression of CD147 in colon cancer tissues was higher than that in normal tissues (P<0.01), and the prognosis of patients with overexpression of CD147 was poor. Western blot analysis showed that the expression of CD147 protein in HCT116 cells was increased compared with that in HCoEPiC cells (P<0.001). Under the fluorescence microscope, the fluorescence expression abundance in the shCD147 low expression group and sh-NON group could reach 90%. Western blot analysis showed that compared with untransfected HCT116 cells, the expression of CD147 protein in the shCD147 group was decreased (P<0.001). After down-regulation of CD147, cell proliferation and colony formation in the sh-CD147 group were decreased compared with untransfected HCT116 cells. The results of Hoechst 33342 staining showed that the nuclear staining of apoptosis in the shCD147 group was stronger and the fluorescence was brighter than that of untransfected HCT116 cells (P<0.001). Compared with untransfected HCT116 cells, the expression of p-MAPK, C-MYC, and BCL-2 in the sh-CD147 group was decreased, but the expression of BAX was increased. Correlation analysis based on the TIMER 2.0 database showed that the expression level of BSG in colon cancer tissue was positively correlated with the expression of MAPK1, MYC, and BCL-2, but negatively correlated with the expression of BAX (P<0.001).

Down-regulation of CD147 expression reduces the proliferation of colon cancer cells and promotes apoptosis, which may be related to the inhibition of the MAPK signaling pathway.