抑郁发作期双相障碍患者外周血5-HTR1A启动子区甲基化水平与抑郁发作的相关性

doi:10.3877/cma.j.issn.1674-0785.2020.04.001

中华临床医师杂志(电子版) ›› 2020, Vol. 14 ›› Issue (04) : 241 -244. doi: 10.3877/cma.j.issn.1674-0785.2020.04.001

所属专题：文献；

临床研究

抑郁发作期双相障碍患者外周血5-HTR1A启动子区甲基化水平与抑郁发作的相关性

于妍¹, 杜启峰¹^,^†(

), 赵存友², 李淑芬², 张岱威¹, 蒋廷云¹

^1. 528451　中山市第三人民医院情感障碍科
^2. 510515　广州，南方医科大学遗传教研室

收稿日期:2020-02-18 出版日期:2020-04-15
通信作者: 杜启峰
基金资助:
广东省医学科研基金项目(A2018394)

5-HTR1A promoter methylation levels in peripheral blood cells of patients with depressive episode of bipolar disorder and correlation with depressive episode

Yan Yu¹, Qifeng Du¹^,^†(), Cunyou Zhao², Shufen Li², Daiwei Zhang¹, Tingyun Jiang¹

^1. Department of Affective Disorder, the Third People's Hospital of Zhongshan, Zhongshan 528451, China
^2. Department of Genetics, Southern Medical University, Guangzhou 510515, China

Received:2020-02-18 Published:2020-04-15
Corresponding author: Qifeng Du
About author:
Corresponding author: Du Qifeng, Email: 547050904@qq.com

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引用本文：

于妍, 杜启峰, 赵存友, 李淑芬, 张岱威, 蒋廷云. 抑郁发作期双相障碍患者外周血5-HTR1A启动子区甲基化水平与抑郁发作的相关性[J]. 中华临床医师杂志(电子版), 2020, 14(04): 241-244.

Yan Yu, Qifeng Du, Cunyou Zhao, Shufen Li, Daiwei Zhang, Tingyun Jiang. 5-HTR1A promoter methylation levels in peripheral blood cells of patients with depressive episode of bipolar disorder and correlation with depressive episode[J]. Chinese Journal of Clinicians(Electronic Edition), 2020, 14(04): 241-244.

目的

探讨处于抑郁发作期的双相障碍患者外周血细胞五羟色胺1A受体基因（5-HTR1A）启动子区甲基化水平以及与抑郁症状的关系。

方法

收集2017年8月至2019年8月来自广东省精神卫生中心、中山市第三人民医院和江门市第三人民医院的65例处于抑郁发作期的双相障碍患者（研究组）和59名健康人（对照组）的外周血，对2组血细胞5-HTR1A启动子区甲基化水平使用焦磷酸测序技术进行检测，采用t检验进行对比。研究组同时进行汉密尔顿抑郁量表（HAMD）评分，采用Pearson相关分析对甲基化水平与量表评分之间的相关性进行分析。

结果

抑郁发作期的双相障碍患者外周血细胞5-HTR1A启动子区甲基化水平低于对照组[（46.86±6.75）% vs （50.03±8.85）%]，差异具有统计学意义（t=2.255，P=0.013）。Pearson相关分析显示研究组HAMD评分与其甲基化率呈负相关（r=-0.631，P<0.01）。

结论

抑郁发作期的双相障碍患者外周血的5-HTR1A启动子区甲基化水平与抑郁发作之间可能存在相关性。

关键词: 双相障碍, 抑郁发作, DNA甲基化

Objective

To investigate the levels of 5-hydroxytryptamine receptor 1A (5-HTR1A) promoter methylation in peripheral blood of patients with bipolar disorder during their depressive episode, and to explore the relationship between the methylation levels and depressive symptoms of the patients.

Methods

Blood samples were collected from 65 patients (study group) with bipolar disorder who were in the stage of depressive episode and 59 healthy people (control group) from August 2017 to August 2018 at Mental Health Center of Guangdong, the Third People's Hospital and Jiangmen City People's Hospital. Methylation levels in the 5-HTR1A promoter region in peripheral blood cells were detected and compared by pyrosequencing. In the study group, the Hamilton depression scale score was used to assess the patients′ depressive level and correlation analysis was conducted between methylation levels and the Hamilton depression scale scores.

Results

The methylation level of 5-HTR1A promoter region in peripheral blood was significantly reduced in the study group than in the control group [(46.86±6.75)% vs (50.03±8.85)%, t=2.255, P=0.013] . A negative correlation was found between Hamilton depression scale scores and methylation levels in the study group (r=-0.631, P<0.01).

Conclusion

There is a correlation between depressive episode and the methylation levels of 5-HTR1A promoter region in peripheral blood of patients with bipolar disorder.

Key words: Bipolar disorder, Epigenetics, DNA methylation

表1 2组研究对象一般临床资料的比较

组别	例数	年龄（岁，±s）	性别（男/女）	受教育年限（年，±s）
研究组	65	37.42±11.90	35/30	15.91±5.34
对照组	59	40.15±11.88	30/29	17.04±3.38
统计值	?	t=1.277	χ²=0.112	t=1.392
P值	?	0.102	0.857	0.083

表1 2组研究对象一般临床资料的比较

组别	例数	年龄（岁，±s）	性别（男/女）	受教育年限（年，±s）
研究组	65	37.42±11.90	35/30	15.91±5.34
对照组	59	40.15±11.88	30/29	17.04±3.38
统计值	?	t=1.277	χ²=0.112	t=1.392
P值	?	0.102	0.857	0.083

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