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中华临床医师杂志(电子版) ›› 2017, Vol. 11 ›› Issue (15) : 2077 -2082. doi: 10.3877/cma.j.issn.1674-0785.2017.15.004

所属专题: 文献

临床论著

Ⅱ型多磷酸肌醇4-磷酸酶、蛋白激酶B在食管鳞癌组织中表达的临床意义及其相关性
张龙1, 郭石平2,()   
  1. 1. 030001 太原,山西医科大学
    2. 030001 太原,山西省肿瘤医院胸外二科
  • 收稿日期:2017-04-05 出版日期:2017-08-01
  • 通信作者: 郭石平

Clinical significance of expression of inositol polyphosphate-4-phosphatase type Ⅱ and Akt in esophageal squamous cell carcinoma

Long Zhang1, Shiping Guo2,()   

  1. 1. Shanxi Medical University, Taiyuan 030001, China
    2. Department of Thoracic Surgery, Tumour Hospital of Shanxi Province, Taiyuan 030001, China
  • Received:2017-04-05 Published:2017-08-01
  • Corresponding author: Shiping Guo
  • About author:
    Corresponding author: Guo Shiping, Email:
引用本文:

张龙, 郭石平. Ⅱ型多磷酸肌醇4-磷酸酶、蛋白激酶B在食管鳞癌组织中表达的临床意义及其相关性[J]. 中华临床医师杂志(电子版), 2017, 11(15): 2077-2082.

Long Zhang, Shiping Guo. Clinical significance of expression of inositol polyphosphate-4-phosphatase type Ⅱ and Akt in esophageal squamous cell carcinoma[J]. Chinese Journal of Clinicians(Electronic Edition), 2017, 11(15): 2077-2082.

目的

探讨Ⅱ型多磷酸肌醇4-磷酸酶(INPP4B)及蛋白激酶B(Akt)在食管鳞癌组织中表达的临床意义及其相关性。

方法

采用实时荧光定量PCR(qRT-PCR)检测32例食管癌患者的癌组织及相应的癌旁组织中(距癌组织>5 cm)中INPP4B mRNA的表达情况,并分析其与食管癌临床病理特征之间的关系。采用免疫组织化学SP法检测70例食管癌组织及20例正常食管上皮组织中INPP4B、Akt蛋白的表达情况,并分析与食管癌临床病理特征的关系及其之间的相关性。

结果

与癌旁组织相比,32例食管癌组织中INPP4B mRNA表达水平上调的比例为65.5%(P<0.05)。INPP4B mRNA的表达水平与食管癌患者的年龄、性别、肿瘤直径、有无淋巴结转移及TNM分期均无相关性(P>0.05)。在70例食管癌组织中及20例正常食管上皮组织中,INPP4B蛋白的阳性表达率显著高于正常食管组织(67.1% vs 40.0%,P<0.05),Akt蛋白的阳性表达率显著高于正常食管组织(72.8% vs 25.0%,P<0.05)。INPP4B蛋白的表达水平与食管癌患者的年龄、性别及临床病理特征无明显相关(均P>0.05);Akt蛋白的表达水平与肿瘤直径、分化程度、有无淋巴结转移及TNM分期均有关(均P<0.05)。INPP4B与Akt蛋白表达呈正相关(r=0.531,P=0.000)。

结论

INPP4B在食管鳞癌组织中的表达上调,INPP4B可能通过激活Akt的活化从而在食管癌中起着癌基因的作用,其可能成为食管癌靶向治疗的一个新的靶点。

Objective

To investigate the expression of inositol polyphosphate-4-phosphatase type Ⅱ (INPP4B) and Akt in esophageal squamous cell carcinoma (ESCC) and to explore their correlation with clinicopathologic factors.

Methods

The expression of INPP4B mRNA in 32 ESCC tissues was detected by quantitative real-time PCR (qRT-PCR). The relationship between INPP4B expression and clinical and pathological characteristics was then analyzed. Immunohistochemistry was performed to detect the expression of INPP4B and Akt proteins in 70 specimens of ESCC and 20 specimens of normal esophageal tissues. The relationship of their expression with clinicopathologic factors was analyzed.

Results

INPP4B mRNA expression was significantly higher in 32 ESCC tissues than in adjacent normal tissues (P<0.05). The expression of INPP4B mRNA was not related to tumor differentiation, gender, age, tumor size, lymph node metastasis, or TNM stage in ESCC (P>0.05). In 70 ESCC tissues and 20 normal esophageal tissues, the rates of INPP4B and Akt expression were significantly higher in ESCC tissue than in normal esophageal tissue (INPP4B: 67.1% vs 40.0%, P<0.05; Akt: 72.8% vs 25.0%, P<0.05). INPP4B protein expression was not correlated with tumor differentiation, lymph node metastasis, or TNM stage. Akt expression was correlated with tumor size, tumor differentiation, lymph node metastasis, and TNM stage. INPP4B expression was positively correlated with Akt expression (r = 0.531, P = 0.000) in ESCC.

Conclusion

INPP4B expression is up-regulated in ESCC at both the mRNA and protein levels. INPP4B may act as an oncogene through promoting the activation of Akt in ESCC. INPP4B may be a new therapeutic target for ESCC.

表1 INPP4B mRNA表达与食管鳞癌临床病理特征之间的关系
表2 INPP4B、Akt在食管鳞癌及食管正常组织中的表达
图2 蛋白激酶B在食管鳞癌组织中的阳性表达(SP,×100)
表3 INPP4B、Akt在食管鳞癌中的表达情况与临床病理特征之间的关系
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