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中华临床医师杂志(电子版) ›› 2017, Vol. 11 ›› Issue (18) : 2237 -2240. doi: 10.3877/cma.j.issn.1674-0785.2017.18.006

所属专题: 文献

基础论著

慢性皮肤溃疡癌变与癌基因RasC-myc异常表达的关系
沈锐1,(), 张金明2, 张凤刚3, 陈平1, 杜学亮1, 黎咏璇1, 刘必来1, 邓林1, 宋红梅4, 徐路生4   
  1. 1. 528000 佛山,广东省佛山市第一人民医院整形美容科
    2. 450120 广州,中山大学附属第二医院整形外科
    3. 528000 佛山,广东省佛山市第一人民医院烧伤整形外科
    4. 528000 佛山,广东省佛山市第一人民医院手足整形外科
  • 收稿日期:2017-03-09 出版日期:2017-09-15
  • 通信作者: 沈锐
  • 基金资助:
    广东省医学科研基金资助项目(A2012637)

Relationship between pathogenesis of chronic skin ulcer and overexpression of Ras and C-myc

Rui Shen1,(), Jingming Zhang2, Fenggang Zhang3, Ping Chen1, Xueliang Du1, Yongxuan Li1, Bilai Liu1, lin Deng1, Hongmei Song4, Lusheng Xu4   

  1. 1. Department of Plastic and Cosmetic Surgery, First People’s Hospital of Foshan, Foshan 528000, China
    2. Department of Plastic Surgery, Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 450052, China
    3. Department of Burn and plastic Surgery, First People’s Hospital of Foshan, Foshan 528000, China
    4. Department of Hand and Foot Plastic Surgery, First People’s Hospital of Foshan, Foshan 528000, China
  • Received:2017-03-09 Published:2017-09-15
  • Corresponding author: Rui Shen
  • About author:
    Corresponding author: Shen Rui, Email:
引用本文:

沈锐, 张金明, 张凤刚, 陈平, 杜学亮, 黎咏璇, 刘必来, 邓林, 宋红梅, 徐路生. 慢性皮肤溃疡癌变与癌基因RasC-myc异常表达的关系[J]. 中华临床医师杂志(电子版), 2017, 11(18): 2237-2240.

Rui Shen, Jingming Zhang, Fenggang Zhang, Ping Chen, Xueliang Du, Yongxuan Li, Bilai Liu, lin Deng, Hongmei Song, Lusheng Xu. Relationship between pathogenesis of chronic skin ulcer and overexpression of Ras and C-myc[J]. Chinese Journal of Clinicians(Electronic Edition), 2017, 11(18): 2237-2240.

目的

探讨RasC-myc癌基因异常表达与慢性皮肤溃疡癌变的关系。

方法

选取10例Marjolin溃疡鳞状细胞癌组织标本。选取10例慢性皮肤溃疡伴假上皮瘤样增生组织标本,选取10例原发性皮肤鳞状细胞癌组织标本,选取5例正常皮肤组织标本。对标本中RasC-myc癌基因表达进行免疫组化分析。

结果

Marjolin溃疡中鳞状细胞癌组织中,散在癌细胞RasC-myc阳性表达,为核染色,而正常组织以及原发性皮肤鳞状细胞癌组织两癌基因均阴性表达,另外,癌基因RasC-myc在慢性皮肤溃疡伴假上皮瘤样组织中不典型增生的上皮脚部位,也呈阴性表达。

结论

RasC-myc的表达异常与慢性皮肤溃疡癌变成鳞状细胞癌有关。

Objective

To explore the relationship between the abnormal overexpression of Ras and C-myc in chronic ulcers of the skin and the pathogenesis of squamous cell carcinoma (SCC) in Marjolin′s ulcer.

Methods

Ten tissue specimens of SCC in Marjolin′s ulcer, ten tissue specimens of chronic skin ulcer accompanied with pseudoepitheliomatous hyperplasia, ten tissue specimens of primary SCC, and five tissue specimens of normal skin were collected. The expression of Ras and C-myc in these specimens was detected by immunohistochemistry.

Results

Both Ras and C-myc were overexpressed in the tissues of SCC in Marjolin′s ulcer. The nuclear staining of Ras and C-myc was positive in some carcinoma cells. However, they were negatively expressed in normal skin tissue and primary SCC cells. They were also not expressed in the epithelial cells with atypical hyperplasia in chronic skin ulcer accompanied with pseudoepitheliomatous hyperplasia.

Conclusion

The abnormal expression of Ras and C-myc is closely related to the carcinogenesis in chronic ulcer.

图1 RasC-myc癌基因在Marjolin溃疡鳞癌、皮肤溃疡并假上皮瘤样增生、原发皮肤鳞癌、正常皮肤组织中的不同的表达情况(H&E染色×200)。图1a为Ras在Marjolin溃疡鳞癌部分细胞表达阳性;图1b为C-myc在Marjolin溃疡鳞癌部分细胞表达阳性;图1c为Ras在皮肤溃疡并假上皮瘤样增生组织中表达阴性;图1d为C-myc在皮肤溃疡并假上皮瘤样增生组织中表达阴性;图1e为Ras在原发鳞癌中表达阴性;图1f为C-myc在原发鳞癌中表达阴性;图1g为Ras在正常皮肤组织表达阴性;图1h为C-myc在正常皮肤组织中表达阴性
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