内蒙古地区健康成年大鼠血清甲状腺激素参考值范围

doi:10.3877/cma.j.issn.1674-0785.2018.06.005

中华临床医师杂志(电子版) ›› 2018, Vol. 12 ›› Issue (06) : 337 -340. doi: 10.3877/cma.j.issn.1674-0785.2018.06.005

所属专题：文献；

基础研究

内蒙古地区健康成年大鼠血清甲状腺激素参考值范围

刘友胜¹, 金山¹^,^†(

), 艾静雯¹, 白银宝¹, 塔拉¹, 乌云图¹, 成格乐¹, 勇宏¹

^1. 010050　呼和浩特，内蒙古医科大学；内蒙古医科大学附属医院普外科

收稿日期:2018-03-01 出版日期:2018-03-15
通信作者: 金山
基金资助:
国家自然科学基金(81460157)

Reference range of serum thyroid hormones in healthy adult rats in Inner Mongolia Autonomous Region

Yousheng Liu¹, Shan Jin¹^,^†(), Jingwen Ai¹, Yinbao Bai¹, La Ta¹, Yuntu Wu¹, Gele Cheng¹, Hong Yong¹

^1. Inner Mongolia Medical University; Department of General Surgery, Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010050, China

Received:2018-03-01 Published:2018-03-15
Corresponding author: Shan Jin
About author:
Corresponding author: Jin Shan, Email: jinshangood@163.com

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目的

建立内蒙古地区大鼠血清甲状腺激素水平的正常参考值范围。

方法

通过严格控制实验条件，分别从内蒙古医科大学实验动物中心筛选出50只正常SPF级Wistar大鼠并经饲养6周，体质量达（200±11）g后收集血清，并对甲状腺组织进行病理学检查，排除各类甲状腺疾病；ELISA法测定血清甲状腺激素水平，用Shapiro-Wilk法进行正态性检验，对符合正态分布的数据应用±s进行描述，利用正态分布法确定本实验的医学参考值范围。

结果

内蒙古地区Wistar大鼠血清甲状腺激素（T3，T4和TSH）水平正常参考值分别为：0.73~0.99 ng/ml、4.02~7.91 ug/dl、0.72~1.44 μIU/ml。

结论

内蒙古地区成年大鼠血清甲状腺激素水平的正常参考值成功建立，可为本地区大鼠甲状腺激素水平提供了基础性数据。

关键词: Wistar大鼠, 甲状腺激素, 正常值

Objective

To establish the reference range of serum thyroid hormones in rats in Inner Mongolia area.

Methods

By strictly controlling experimental conditions, 50 normal Wistar rats of SPF grade were screened from the Experimental Animal Center of Inner Mongolia Medical University and fed for 6 weeks. Then, serum samples were collected after the rats weighed (200±11) g and pathological examination of thyroid tissue excluded all types of thyroid disease. Serum thyroid hormone levels were determined by ELISA, and a normality test was performed by the Shapiro-Wilk method. Data following a normal distribution are expressed mean ± standard deviation, and the medical reference range was determined by a normal distribution method.

Results

The normal reference values of serum T3, T4 and TSH levels in Wistar rats in this region were 0.73-0.99 ng/mL, 4.02-7.91 μg/dL and 0.72-1.44 μIU/mL, respectively.

Conclusion

The normal reference values of serum thyroid hormone levels in adult rats in Inner Mongolia have been successfully established, which provided basic data for thyroid hormone levels in rats in this region.

Key words: Wistar rats, Thyroid hormone, Normal reference

图1 大鼠甲状腺切片（HE染色4×10）

表1 Wistr大鼠血清甲状腺激素实验结果

指标	±s	范围	95%CI
T3（ng/ml)	0.86±0.07	0.73~0.99	0.84~0.88
T4（μg/dl)	5.97±0.99	4.02~7.91	5.69~6.25
TSH（μIU/ml)	1.05±0.14	0.72~1.44	1.01~1.09

表1 Wistr大鼠血清甲状腺激素实验结果

指标	±s	范围	95%CI
T3（ng/ml)	0.86±0.07	0.73~0.99	0.84~0.88
T4（μg/dl)	5.97±0.99	4.02~7.91	5.69~6.25
TSH（μIU/ml)	1.05±0.14	0.72~1.44	1.01~1.09

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