IL-27、TNF-α和5-HT在CIA共病抑郁症大鼠模型中的表达特点

doi:10.3877/cma.j.issn.1674-0785.2019.04.009

目的

探讨肿瘤坏死因子-α（TNF-α）、白介素-27（IL-27）和5-羟色胺（5-HT）在胶原诱导的关节炎（CIA）共病抑郁症大鼠模型中的表达特点。

方法

通过复合造模思路，在Ⅱ型CIA大鼠模型基础上联合慢性不可预知温和刺激抑郁症模型，建立类风湿关节炎（RA）共病抑郁症大鼠模型。32只雌性Wistar大鼠随机分为4组：（1）正常对照组：每笼4只，正常饲养；（2）RA组：每笼4只，建模后正常饲养；（3）抑郁症组：每笼1只，与RA共病抑郁症组同时给予慢性不可预知温和刺激方法；（4）RA共病抑郁症组：每笼饲养1只，关节炎模型建立1周后，给予慢性不可预知温和刺激建立。大鼠处死后腹主动脉采血，采用酶联免疫吸附法测定血清TNF-α、IL-27和5-HT水平。采用方差分析比较组间差异，LSD-t检验进行组间两两比较。

结果

TNF-α和IL-27水平在RA共病抑郁症组均高于正常对照组［（297.97±34.23）ng/L vs （142.31±13.24）ng/L；（297.97±34.73）ng/L vs （212.43±14.52）ng/L］，差异具有统计学意义（t=3.41，P=0.021；t=4.78，P=0.029）。5-HT水平在RA共病抑郁症组低于正常对照组［（108.54±11.51） vs （241.23±13.48）ng/L］，差异具有统计学意义（t=7.821，P=0.017）。

结论

RA共病抑郁症大鼠模型中TNF-α、IL-27水平升高同时伴有5-HT水平降低，提示TNF-α、IL-27和5-HT可能参与了RA共病抑郁症的发病过程，可以作为临床上判断疾病进展及治疗效果的重要参考指标。

关键词: 关节炎，类风湿, 抑郁症, 细胞因子

Objective

To investigate the expression of tumor necrosis factor (TNF)-α, interleukin (IL)-27, and 5-hydroxytryptamine (5-HT) in collagen-induced arthritis (CIA) comorbid with depression.

Methods

A rat model of rheumatoid arthritis (RA) comorbid with depression was established by subjecting type II CIA rats to chronic unpredictable mild stress (CUMS). Thirty-two female Wistar rats were randomly divided into four groups: normal group (four rats per cage, normal feeding), RA group (four rats per cage, normal feeding after modeling), depression group (one rat per cage, CUMS was given to rats), and RA comorbid with depression group (one rat in each cage, CUMS was given one week after the arthritis model was established). Serum TNF-α, IL-27, and 5-HT levels were analyzed by enzyme-linked immunosorbent assay. One-way ANOVA and LSD-t test were used for the overall comparison between groups.

Results

The levels of TNF-α and IL-27 in the model group were significantly higher than those in the control group [(297.97±34.23) ng/L vs (142.31±13.24) ng/L, t=3.41, P=0.021; (297.97±34.73) ng/L vs (212.43±14.52) ng/L, t=4.78, P=0.029]. The levels of 5-HT in the model group was significantly lower than that in the control group [(108.54±11.51) ng/L vs (241.23±13.48) ng/L, t=7.821, P=0.017].

Conclusion

The increase of TNF-α and IL-27 levels and the decrease of 5-HT levels in rats with RA comorbid with depression suggest that TNF- α, IL-27, and 5-HT might be involved in the pathogenesis of RA comorbid with depression. They can be used as important reference indexes to judge the progression of the disease and the therapeutic effect.

Key words: Arthritis, rheumatoid, Depression, Cytokines

表1 各组大鼠TNF-α、IL-27和5-HT水平比较（ng/ml，±s）

组别	数量	TNF-α	IL-27	5-HT
正常组	8	142.31±13.24	212.43±14.52	241.23±13.48
RA组	8	220.15±16.38	258.67±23.17	232.65±14.17
抑郁症组	8	214.83±14.71	238.45±33.08	158.36±12.65
RA共病抑郁症组	8	254.16±19.23^a	297.97±34.73^b	108.54±11.51^c
F值	?	19.62	18.41	12.31
P值	?	<0.001	<0.001	0.023

表1 各组大鼠TNF-α、IL-27和5-HT水平比较（ng/ml，±s）

组别	数量	TNF-α	IL-27	5-HT
正常组	8	142.31±13.24	212.43±14.52	241.23±13.48
RA组	8	220.15±16.38	258.67±23.17	232.65±14.17
抑郁症组	8	214.83±14.71	238.45±33.08	158.36±12.65
RA共病抑郁症组	8	254.16±19.23^a	297.97±34.73^b	108.54±11.51^c
F值	?	19.62	18.41	12.31
P值	?	<0.001	<0.001	0.023

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Expression characteristics of TNF-α, IL-2, and 5-HT in a model of collagen-induced arthritis comorbid with depression

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Expression characteristics of TNF-α, IL-2, and 5-HT in a model of collagen-induced arthritis comorbid with depression