切换至 "中华医学电子期刊资源库"

中华临床医师杂志(电子版) ›› 2021, Vol. 15 ›› Issue (09) : 666 -671. doi: 10.3877/cma.j.issn.1674-0785.2021.09.005

临床研究

早期梅毒患者血清IL-1β、IL-27、IL-33水平与细胞免疫状况的关系
吴远慧1, 朱由瑾1, 周向昭1,(), 温杰1, 高晓莉1, 冯冬梅1   
  1. 1. 075000 河北张家口,河北北方学院附属第一医院皮肤科
  • 收稿日期:2021-04-16 出版日期:2021-09-15
  • 通信作者: 周向昭
  • 基金资助:
    河北省2020年政府资助人才项目计划

Relationship of serum IL-1β, IL-27 and IL-33 with cellular immune status in patients with early syphilis

Yuanhui Wu1, Youjin Zhu1, Xiangzhao Zhou1,(), Jie Wen1, Xiaoli Gao1, Dongmei Feng1   

  1. 1. Department of Dermatology, the First Affiliated Hospital of Hebei Northern University, Zhangjiakou 075000, China
  • Received:2021-04-16 Published:2021-09-15
  • Corresponding author: Xiangzhao Zhou
引用本文:

吴远慧, 朱由瑾, 周向昭, 温杰, 高晓莉, 冯冬梅. 早期梅毒患者血清IL-1β、IL-27、IL-33水平与细胞免疫状况的关系[J/OL]. 中华临床医师杂志(电子版), 2021, 15(09): 666-671.

Yuanhui Wu, Youjin Zhu, Xiangzhao Zhou, Jie Wen, Xiaoli Gao, Dongmei Feng. Relationship of serum IL-1β, IL-27 and IL-33 with cellular immune status in patients with early syphilis[J/OL]. Chinese Journal of Clinicians(Electronic Edition), 2021, 15(09): 666-671.

目的

探讨早期梅毒患者血清白细胞介素(IL)-1β、IL-27、IL-33水平与细胞免疫功能指标的相关性。

方法

选择2018年2月至2020年6月河北北方学院附属第一医院收治的73例早期梅毒患者(早期梅毒组)、69例中晚期梅毒患者(中晚期梅毒组)和50例健康志愿者(对照组)。根据快速血浆反应素环状卡片试验(RPR)将早期梅毒组患者分为高RPR滴度组(>1∶4)32例,低RPR滴度组(≤1∶4)41例。检测各组受试者血清IL-1β、IL-27、IL-33水平以及外周血T细胞、NK细胞占比,分析早期梅毒患者血清IL-1β、IL-27、IL-33水平与细胞免疫功能指标的相关性。

结果

早期梅毒组、中晚期梅毒组患者血清IL-1β、IL-33水平高于对照组(P均<0.05),血清IL-27水平低于对照组(P<0.05)。高RPR滴度组患者血清IL-1β、IL-33水平高于低RPR滴度组(P均<0.05),血清IL-27水平低于低RPR滴度组(P<0.05)。早期梅毒组患者治疗后血清IL-1β、IL-33水平下降(P均<0.05),血清IL-27水平上升(P<0.05)。早期梅毒患者血清IL-1β、IL-33水平与CD3+T细胞、CD4+T细胞、CD4+/CD8+、CD3-CD56+NK细胞、CD56brightNK细胞、CD56dimNK细胞占比呈负相关关系(IL-1β:r=-0.819、-0.735、0.773、-0.842、-0.742、-0.793、IL-33:r=-0.802、-0.815、-0.792、-0.863、-0.802、-0.786,P均<0.05),与CD8+T细胞呈正相关关系(r=0.764、0.751,P均<0.05);IL-27与CD3+T细胞、CD4+T细胞、CD4+/CD8+、CD3-CD56+NK细胞、CD56brightNK细胞、CD56dimNK细胞占比呈正相关关系(r=0.737、0.857、0.769、0.812、0.825、0.809,P均<0.05),与CD8+T细胞呈负相关关系(r=-0.738,P<0.05)。

结论

早期梅毒患者血清IL-1β、IL-33水平升高,IL-27水平降低,其可能通过抑制细胞免疫功能参与早期梅毒的发病过程。

Objective

To investigate the correlation of serum interleukin (IL)-1β, IL-27, and IL-33 with cellular immunity in patients with early syphilis.

Methods

Seventy-three syphilis patients with early syphilis, 69 patients with middle-late syphilis (course of infection ≥1 year), and 50 healthy volunteers (control group) were selected at the First Affiliated Hospital of Hebei Northern University from February 2018 to June 2020. According to the rapid plasma reacting ring card test (RPR), the patients in the early syphilis group were divided into either a high RPR titer group (>1∶4) (n=32) or a low RPR titer group (≤1∶4) (n=41). Serum levels of IL-1β, IL-27, and IL-33 and the percentage of T cells and NK cells were determined, and the correlation of serum IL-1β, IL-27, and IL-33 with immune indexes in patients with early syphilis was analyzed.

Results

The levels of IL-1β and IL-33 in the early syphilis group and middle-late syphilis group were significantly higher than those in the control group (P<0.05 each), and the level of IL-27 was significantly lower than that of the control group (P<0.05). The levels of IL-1β and IL-33 in the high RPR titer group were significantly higher than those in the control group and low RPR titer group (P<0.05 each), and the level of IL-27 was significantly lower than that in the low RPR titer group (P<0.05). The levels of serum IL-1β and IL-33 in the syphilis group decreased after treatment (P<0.05 each), while the level of serum IL-27 increased after treatment (P<0.05). Serum IL-1β and IL-33 in early syphilis patients were negatively correlated with CD3+ T cells, CD4+ T cells, CD4+/CD8+, CD3-CD56+ NK cells, CD56brightNK cells, and CD56dimNK cells (IL-1β: r=-0.819, -0.735, 0.773, -0.842, -0.742, and -0.793, respectively; IL-33: r=-0.802, -0.815, -0.792, -0.863, -0.802, and -0.786, respectively; P<0.05 for all), and positively correlated with CD8+ T cells (r=0.764 and 0.751, respectively; P<0.05 for both). Serum IL-27 levels were positively correlated with the levels of CD3+T cells, CD4+ T cells, CD4+/CD8+, CD3-CD56+ NK cells, CD56brightNK cells, and CD56dimNK cells (r=0.737, 0.857, 0.769, 0.812, 0.825, and 0.809; P<0.05 for all), and negatively correlated with CD8+ T cells (r=-0.738, P<0.05).

Conclusion

Serum levels of IL-1β and IL-33 in patients with early syphilis increase, while the levels of IL-27 and IL-1β decrease in such patients. The decrease of IL-27 may participate in the pathogenesis of early syphilis by inhibiting cellular immunity.

表1 各组受试者血清IL-1β、IL-27、IL-33水平和细胞免疫功能指标比较(
xˉ
±s
表2 不同RPR滴度的早期梅毒患者血清IL-1β、IL-27、IL-33水平和细胞免疫功能指标比较(
xˉ
±s
表3 早期梅毒患者血清IL-1β、IL-27、IL-33水平与细胞免疫功能指标的相关性
1
龚向东, 蒋娟, 苏晓红. 我国梅毒流行形势与控制对策探讨 [J]. 中华皮肤科杂志, 2014, 47(5): 307-309.
2
张伟, 王政洁, 闫越颖, 等. Tim-3对早期梅毒患者外周血CD56 dim NK细胞的影响及初步机制 [J]. 中国免疫学杂志, 2019, 35(16): 2005-2010.
3
梁丽贞, 王俊, 张艳, 等. 神经梅毒患者外周血和脑脊液Th1/Th2型细胞因子及CXCL13的临床价值 [J]. 中华临床感染病杂志, 2018, 11(2): 102-107.
4
Blom L, Poulsen LK. IL-1 family members IL-18 and IL-33 upregulate the inflammatory potential of differentiated human Th1 and Th2 cultures [J]. J Immunol, 2012, 189(9): 4331-4337.
5
Jones GW, Hill DG, Cardus A, et al. IL-27: a double agent in the IL-6 family [J]. Clin Exp Immunol, 2018, 193(1): 37-46.
6
焦书竹. 梅毒的诊断与治疗 [J]. 中国实用妇科与产科杂志, 1998, 14(2): 13-15.
7
樊尚荣, 梁丽芬. 2015年美国疾病控制中心性传播疾病诊断和治疗指南(续)-梅毒的诊断和治疗指南 [J]. 中国全科医学, 2015, 18(27): 3260-3264.
8
Radolf JD, Deka RK, Anand A, et al. Treponema pallidum, the syphilis spirochete: making a living as a stealth pathogen [J]. Nat Rev Microbiol, 2016, 14(12): 744-759.
9
唐利利, 程玉燕, 高金平, 等. 梅毒免疫学机制研究进展 [J]. 中国麻风皮肤病杂志, 2017, 33(5): 317-320.
10
高晓莉, 武铮, 王剑锋, 等. 梅毒的细胞免疫学发病机制研究进展 [J]. 中国艾滋病性病, 2020, 26(10): 1144-1146.
11
Lopez-Castejon G, Brough D. Understanding the mechanism of IL-1β secretion [J]. Cytokine Growth Factor Rev, 2011, 22(4): 189-195.
12
张跃军, 蒋传好, 刘良专, 等. 梅毒螺旋体膜蛋白Tp0971经TLR2/NF-κB诱导巨噬细胞分泌细胞因子 [J]. 中国免疫学杂志, 2017, 33(5): 668-672.
13
Kay AB, Clark P, Maurer M, et al. Elevations in T-helper-2-initiating cytokines (interleukin-33, interleukin-25 and thymic stromal lymphopoietin) in lesional skin from chronic spontaneous ('idiopathic') urticaria [J]. Br J Dermatol, 2015, 172: 1294-1302.
14
Zheng D, Yang X. Clinical observation on the therapeutic effect of desloratadine citrate disodium in the treatment of chronic urticaria and changes in IL4, IL18, IL23 and IL-33 levels before and after treatment [J]. Pak J Pharm Sci, 2017, 30: 1139-1142.
15
孟晓莹, 柴立民, 王长志, 等. 黄芪、当归、忍冬藤水提冻干粉组合对白细胞介素1β诱导大鼠T细胞亚群增殖与分化的影响 [J]. 环球中医药, 2019, 12(8): 1154-1159.
16
Lin W, Zhou Q, Liu C, et al. Increased plasma IL-17, IL-31, and IL-33 levels in chronic spontaneous urticaria [J]. Sci Rep, 2017, 7(1): 17797.
17
Fabbi M, Carbotti G, Ferrini S. Dual roles of IL-27 in cancer biology and immunotherapy [J]. Mediators Inflamm, 2017, 2017: 3958069.
18
Povroznik JM, Robinson CM. IL-27 regulation of innate immunity and control of microbial growth [J]. Future Sci OA, 2020, 6(7): FSO588.
19
章鹏飞, 季必华. 早期梅毒患者治疗前后血清IL-27和IL-33水平检测 [J]. 皖南医学院学报, 2016, 35(5): 453-456.
20
蒲新露. 早期梅毒患者治疗前后白细胞介素-27、33和γ干扰素的检测及分析 [J]. 实用皮肤病学杂志, 2019, 12(6): 329-332.
21
Yoshida H, Hunter CA. The Immunobiology of Interleukin-27 [J]. Annu Rev Immunol, 2015, 33: 417-443.
22
Choi YH, Lim EJ, Kim SW, et al. IL-27 enhances IL-15/IL-18-mediated activation of human natural killer cells [J]. J Immunother Cancer, 2019, 7(1): 168.
[1] 张艳兰, 徐琳, 王彩英, 杨洪玲, 庞琳. 56例先天性梅毒新生儿的临床特征及预后[J/OL]. 中华实验和临床感染病杂志(电子版), 2024, 18(03): 163-169.
[2] 师璐, 黄宇明, 寇程, 秦开宇, 吴雅丽, 马小扬, 许东梅. 夫妻共同感染梅毒螺旋体临床表型及实验室指标差异[J/OL]. 中华实验和临床感染病杂志(电子版), 2022, 16(05): 328-336.
[3] 许东梅, 马小扬, 黄宇明. 神经梅毒诊疗现状及进展[J/OL]. 中华实验和临床感染病杂志(电子版), 2022, 16(05): 300-306.
[4] 中华医学会器官移植学分会, 国家肾脏移植质控中心. 肾移植受者人类微小病毒B19感染临床诊疗技术规范(2022版)[J/OL]. 中华移植杂志(电子版), 2022, 16(04): 193-200.
[5] 朱丽臻, 范文萃, 李梦瑶, 李丽, 张月, 石艳红. 外周血NLRP3、IL-18和IL-1β在肺炎支原体肺炎患儿中的表达及检测意义[J/OL]. 中华肺部疾病杂志(电子版), 2023, 16(06): 860-863.
[6] 席素雅, 商焕霞, 贺向红, 张玲, 郑立恒, 乔庆哲, 刘薇. IL-27、IFN-γ、IGRA、ADA在结核性胸腔积液中的表达[J/OL]. 中华肺部疾病杂志(电子版), 2023, 16(05): 664-666.
[7] 魏晓辉, 苏智祥, 陈文娟, 张燕军, 刘佳, 丁彩霞, 王云梅, 侯银银. PD-1单抗治疗晚期非小细胞肺癌血清IL-27与预后相关性[J/OL]. 中华肺部疾病杂志(电子版), 2023, 16(05): 650-653.
[8] 吴素馨, 叶韵斌. TCR-T治疗中靶点选择的策略[J/OL]. 中华细胞与干细胞杂志(电子版), 2022, 12(04): 250-256.
[9] 陈伟杰, 何小东. 胆囊癌免疫靶向治疗进展[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 763-768.
[10] 章启铭, 肖爵贤, 程祖珏. 脊柱外伤后表现为脊髓脊膜炎的神经梅毒一例报道[J/OL]. 中华神经创伤外科电子杂志, 2024, 10(03): 186-188.
[11] 姬丽娅, 姬昂, 狄政莉, 熊婧, 刘志勤, 薛秀云, 费晓炜, 豆雅楠, 王利. 叶酸通过NLRP3/ASC/Caspase-1信号通路对HT22细胞拟帕金森损伤作用的研究[J/OL]. 中华神经创伤外科电子杂志, 2022, 08(04): 196-203.
[12] 张钊龙, 郑卉, 赵丹阳, 赵悰怡, 刘之琪, 张优佳, 秦秉玉. 趋化因子CXC配体13在中枢神经系统感染中的意义及相关研究进展[J/OL]. 中华重症医学电子杂志, 2024, 10(01): 54-59.
[13] 胡志恒, 任洪波, 宋志远, 张运刚, 韩晓正. 血清sTIM-3及其配体Gal-9、CEACAM-1与创伤性颅脑损伤患者脑损伤程度及预后的关系[J/OL]. 中华脑科疾病与康复杂志(电子版), 2024, 14(04): 201-207.
[14] 符梅沙, 周玉华, 李慧, 薛春颜. 淋巴细胞免疫治疗对复发性流产患者外周血T淋巴细胞亚群分布与PD1/PD-L1表达的影响及意义[J/OL]. 中华临床医师杂志(电子版), 2023, 17(06): 726-730.
[15] 冯家立, 徐鲁. 提高化学发光法检测梅毒螺旋体准确性和效率的探索[J/OL]. 中华临床实验室管理电子杂志, 2024, 12(02): 86-90.
阅读次数
全文


摘要