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中华临床医师杂志(电子版) ›› 2023, Vol. 17 ›› Issue (04) : 386 -390. doi: 10.3877/cma.j.issn.1674-0785.2023.04.004

临床研究

DDX5在甲状腺癌中的表达及其临床意义
赵雪琴, 苏学峰, 任东伟, 崔冰冰, 王振东(), 李慧   
  1. 041000 山西临汾,临汾市人民医院病理科
    041000 山西临汾,临汾市人民医院院办
    041000 山西临汾,临汾市人民医院甲乳科
    041000 山西临汾,临汾市人民医院临床研究中心
    041000 山西临汾,临汾市人民医院胸外科
    041000 山西临汾,临汾市人民医院智慧医院办公室
  • 收稿日期:2022-02-07 出版日期:2023-04-15
  • 通信作者: 王振东
  • 基金资助:
    山西省卫生计生委科研项目(2017192)

Clinical significance of expression of DDX5 in thyroid cancer

Xueqin Zhao, Xuefeng Su, Dongwei Ren, Bingbing Cui, Zhendong Wang(), Hui Li   

  1. Department of Pathology, Linfen People's Hospital, Linfen 041000, China
    Hospital Office, Linfen People's Hospital, Linfen 041000, China
    Department of Thyroid and Breast Diseases, Linfen People's Hospital, Linfen 041000, China
    Clinical Research Center, Linfen People's Hospital, Linfen 041000, China
    Department of Thoracic Surgery, Linfen People's Hospital, Linfen 041000, China
  • Received:2022-02-07 Published:2023-04-15
  • Corresponding author: Zhendong Wang
引用本文:

赵雪琴, 苏学峰, 任东伟, 崔冰冰, 王振东, 李慧. DDX5在甲状腺癌中的表达及其临床意义[J]. 中华临床医师杂志(电子版), 2023, 17(04): 386-390.

Xueqin Zhao, Xuefeng Su, Dongwei Ren, Bingbing Cui, Zhendong Wang, Hui Li. Clinical significance of expression of DDX5 in thyroid cancer[J]. Chinese Journal of Clinicians(Electronic Edition), 2023, 17(04): 386-390.

目的

探讨DEAD-box家族的DDX5(RNA解旋酶)在甲状腺癌组织中的表达,并分析其临床病理意义。

方法

收集临汾市医联体范围内2014年10月~2021年9月手术治疗切除的甲状腺癌组织标本100例,甲状腺良性病变组织标本100例,并收集患者临床病理学资料;采用免疫组化法与Western blot法检测甲状腺癌及良性病变组织中DDX5的表达情况,并对检测结果进行统计学分析。

结果

甲状腺癌组织中DDX5/β-actin蛋白表达情况显著高于良性组织(30.44±3.71 vs 13.22±2.83,P<0.01);DDX5阳性表达与淋巴结转移、甲状腺癌临床分期显著相关(P<0.05),与甲状腺癌患者的年龄、性别、病理类型无相关性(P>0.05)。

结论

DDX5过表达参与了甲状腺癌的发生与发展。

Objective

To investigate the expression of DEAD-box RNA helicase 5 (DDX5) in thyroid cancer and to analyze its clinical significance.

Methods

Specimens of thyroid cancer (n=100) and benign thyroid lesions (n=100) surgically removed at the hospitals belonging to Linfen Medical Association from October 2014 to September 2021 were selected, and the clinicopathological data of these patients were collected. Western blot and immunohistochemistry were used to detect the expression of DDX5 in thyroid cancer and benign lesions, and the results were statistically analyzed.

Results

The expression of DDX5 protein in thyroid carcinoma was significantly higher than that in benign tissues (30.44±3.71 vs 13.22±2.83, P<0.01). The positive expression of DDX5 was significantly correlated with lymph node metastasis and TNM stage of thyroid cancer (P<0.05), but not with age, gender, or pathological type (P>0.05).

Conclusion

Overexpression of DDX5 might be involved in the occurrence and development of thyroid cancer.

图1 免疫组化检测DDX5在甲状腺癌组织和良性病变组织中的表达(×100) 注:T为甲状腺癌组织;BLT为良性病变组织
表1 DDX5表达与甲状腺癌临床病理特征间的关系
图2 甲状腺癌组织与良性组织DDX5表达Western blot鉴定结果 注:T为甲状腺癌组织;BLT为良性病变组织
图3 甲状腺癌组织与良性病变组织DDX5表达Western blot鉴定灰度值结果。图a为患者1 DDX5的相对表达量;图b为患者2 DDX5的相对表达量 注:Tumor为甲状腺癌组织;BLT为良性病变组织
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