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中华临床医师杂志(电子版) ›› 2017, Vol. 11 ›› Issue (19) : 2280 -2285. doi: 10.3877/cma.j.issn.1674-0785.2017.19.006

所属专题： 文献；

基础论著

阿司匹林对周围神经损伤修复影响的实验性研究
徐连彬1, 常文凯2,†()   
  1. 1. 030001　太原，山西医科大学
    2. 030001　太原，山西医科大学第二医院
  • 收稿日期:2017-02-20 出版日期:2017-10-01
  • 通信作者: 常文凯

Effect of aspirin on repair of peripheral nerve injury in rats

Lianbin Xu1, Wenkai Chang2,†()   

  1. 1. Shanxi Medical University, Taiyuan 030001, China
    2. Shanxi Medical University Second Hospital, Taiyuan 030001, China
  • Received:2017-02-20 Published:2017-10-01
  • Corresponding author: Wenkai Chang
  • About author:
    Corresponding author: Chang Wenkai, Email:
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引用本文：

徐连彬, 常文凯. 阿司匹林对周围神经损伤修复影响的实验性研究[J]. 中华临床医师杂志(电子版), 2017, 11(19): 2280-2285.

Lianbin Xu, Wenkai Chang. Effect of aspirin on repair of peripheral nerve injury in rats[J]. Chinese Journal of Clinicians(Electronic Edition), 2017, 11(19): 2280-2285.

目的

研究阿司匹林对周围神经损伤修复的促进作用。

方法

将30只SD大鼠随机分组。所有大鼠左侧坐骨神经离断，显微镜下行神经吻合术，实验组（A）阿司匹林溶液灌胃，对照组（S）等量0.9%NaCl灌胃。术后2、4、6周分别测量感觉神经再生距离，术后2、4、6、8周分别取双侧腓肠肌称重并计算湿质量比恢复率，术后8周检测大鼠坐骨神经传导速度及潜伏期、计算神经传导恢复率，术后4、8周分别取材行免疫组化S-100染色，观察坐骨神经施万细胞增生情况，应用Olympus软件并计算单位视野中施万细胞的数量，进行统计学分析；术后8周电镜下观察施万细胞增生情况及神经髓鞘排列变化情况。组间比较采用两两之间的t检验，以P<0.05为差异有统计学意义。

结果

感觉神经再生距离：实验组较对照组恢复距离更长，术后2、4、6周差异均具有统计学意义[2周：（16.54±1.43）mm vs （9.73±1.21）mm，t=－8.1313，P<0.001；4周：（18.04±1.08）mm vs （13.20±1.11）mm，t=－13.104，P<0.001；6周：（22.67±1.27）mm vs （15.19±0.57）mm，t=－5.746，P<0.001]。大鼠腓肠肌术侧与健侧湿质量比：术后第2周实验组与对照组比较差异无统计学意义（0.955±0.011 vs 0.944±0.013，t=－1.695，P=0.132），术后第4、6、8周差异均具有统计学意义（4周：0.872±0.005 vs 0.828±0.057，t=－13.104，P<0.001；4周：0.798±0.007 vs 0.764±0.0128，t=－5.746，P<0.001；8周：0.783±0.011 vs 0.74±0.008，t=－6.123，P<0.001）。神经电生理：实验组潜伏期明显短于对照组[（18.71±1.49）ms vs （30.84±1.36） ms，t=－13.695，P<0.001]，波幅、传导速度、运动神经传导速度恢复率明显高于对照组[（5.58±0.55）mV vs （4.42±0.58）mV，t=3.224，P=0.012；（31.99±1.22）m/s vs （21.06±0.78）m/s，t=16.776，P<0.001；（76.5±20.8）% vs （63.9±26.1）%，t=8.370，P<0.001]，差异具有统计学意义（P<0.001）。免疫组化：术后4、8周实验组免疫组化S100B蛋白抗体表达的施万细胞数量均高于同时期对照组，差异具有统计学意义[4周：（442.00±35.36）个 vs （242.80±24.33）个，t=－10.378，P<0.001；8周：（928.00±43.25）个 vs （552.80±65.23）个，t=－10.719，P<0.001]。电镜显示：术后各时间点实验组较对照组髓鞘板层分布较好，厚度较均匀，排列整齐。

结论

阿司匹林对周围神经损伤修复具有促进作用。

关键词: 周围神经损伤, 阿司匹林, 神经再生
Objective

To investigate whether aspirin promotes the repair of peripheral nerve injury.

Methods

Thirty SD rats were randomly divided into two groups: an experimental group (A) and a control group (S). The left sciatic nerve of all rats was severed and anastomosed under a microscope. The rats in group A were treated with aspirin, and those in group S were given the same amount of saline. The sensory nerve regeneration distance was measured at the second, fourth, and sixth weeks after operation. The bilateral gastrocnemius muscle was weighed and the recovery rate of wet weight was calculated at the second, fourth, sixth, and eighth weeks after operation. The sciatic nerve conduction velocity was measured, and the number of Schwann cells in each field of view was calculated using Olympus software. The number of Schwann cells in each visual field was also calculated by immunohistochemical S-100 staining at the fourth and eighth weeks after operation. The proliferation of Schwann cells and the changes of myelin sheath were observed by electron microscopy at the eighth week after operation. Comparison between groups was performed using the t test, and P<0.05 was considered to have statistical difference.

Results

The regeneration distance of sensory nerve was significantly longer in group A than in group S at the second [(16.54±1.43)mm vs (9.73±1.21)mm, t=-8.131 3, P<0.001], fourth [(18.04±1.08)mm vs (13.20±1.1)mm, t=-13.104, P<0.001], and sixth weeks [(22.67±1.27)mm vs (15.19±0.57)mm, t=-5.746, P<0.001] after operation. There was no significant difference in muscle wet weight ratio between groups A and S at the second week postoperatively (0.955±0.011 vs 0.944±0.013, t=-1.695, P=0.132), but a significant difference was observed at the fourth (0.872±0.005 vs 0.828±0.057, t=-13.104, P<0.001), sixth (0.798±0.007 vs 0.764±0.0128, t=-5.746, P<0.001), and eighth weeks (0.783±0.011 vs 0.741±0.008, t=-6.123, P<0.001) after operation. Electrophysiology of group A was significantly superior to that of group S [(31.99±1.22)ms vs (21.06±0.78)ms, t=16.776, P<0.001]. The latency of group A was significantly lower than that of group S [(18.71±1.49)ms vs (30.84±1.36)ms, t=-13.695, P<0.001]. The amplitude of group A was significantly higher than that of group S [(5.58±0.55)mV vs (4.42±0.58)mV, t=3.224, P=0.012]. The recovery rate of conduction velocity in group A was significantly better than that of group S [(76.5±20.8)% vs (63.9±26.1)%, t=8.370, P<0.001]. The number of Schwann cells expressing S-100 protein was significantly higher in group A than in group S at the fourth [(442.00±35.36)cells vs (242.80±24.33)cells, t=-10.378, P<0.001] and eighth weeks [(928.00±43.25)cells vs (552.80±65.23)cells, t=-10.719, P<0.001] after operation. Electron microscopy showed that the distribution of myelin sheath was better in the aspirin group than in the control group at each time point, and the thickness was more uniform and neat.

Conclusion

Aspirin can promote the repair of peripheral nerve injury.

Key words: Peripheral nerve injury, Aspirin, Nerve regeneration
图3 2组大鼠术后8周坐骨神经横切面电镜图像　3a：实验组（枸橼酸铅染色，×40 000）；3b：对照组（×4000）
表1 2组大鼠术后2、4、6周感觉神经再生距离的比较（mm，±s）
组别 动物只数 2周 4周 6周
对照组 5 9.73±1.21 13.20±1.11 15.19±0.57
实验组 5 16.54±1.43 18.04±1.08 22.67±1.27
t值 ? -8.1313 -13.104 -5.746
P值 ? <0.001 <0.001 <0.001
表2 2组大鼠术后2、4、6、8周腓肠肌术侧与健侧湿质量比的比较（5只，±s）
组别 动物只数 2周 4周 6周 8周
对照组 5 0.944±0.011 0.828±0.057 0.764±0.0128 0.741±0.008
实验组 5 0.955±0.013 0.872±0.005 0.798±0.007 0.783±0.011
t值 ? -1.695 -13.104 -5.746 -6.123
P值 ? 0.132 <0.001 <0.001 <0.001
表3 2组大鼠术后8周神经电生理检测结果比较（±s）
组别 动物只数 潜伏期（ms） 波幅（mV） 传导速度（m/s） 运动神经传导速度恢复率（%）
对照组 5 30.84±1.36 4.42±0.58 21.06±0.78 63.9±26.1
实验组 5 18.71±1.49 5.58±0.55 31.99±1.22 76.5±20.8
t值 ? -13.695 3.224 16.776 8.370
P值 ? <0.001 0.012 <0.001 <0.001
表4 2组大鼠术后4、8周S100B蛋白抗体表达施万细胞数量（个，±s）
组别 动物只数 4周 8周
对照组 5 242.80±24.33 552.80±65.23
实验组 5 442.00±35.36 928.00±43.25
t值 ? -10.378 -10.719
P值 ? <0.001 <0.001
图1 2组大鼠术后4、8周免疫组化镜下图像（×400）　1a：对照组4周；1b：实验组4周；1c：对照组8周；1d：实验组8周；蓝色为阳性结果
图2 周围神经损伤大鼠模型的制备　A：离断神经；B：吻接神经
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