探讨羊使用水囊叠加加压法建立改良腹腔高压动物模型。

实验羊5只，麻醉后沿中线切开6.0 cm小口，预置缝线，先置入空加压水囊，再置入基础水囊6个（共计1200 ml），无张力下缝线打结关腹。再经过引流管向加压水囊中注入0.9%氯化钠溶液，增加腹腔容积，制作动物模型；每注入50 ml 0.9%氯化钠溶液，测压1次，绘制腹腔压力-容积曲线；调定腹腔内压力40~50 cmH₂O，完成动物模型制作。术后72 h，处死实验动物，取出肝、肾，用10%甲醛溶液固定24 h后常规石蜡包埋，切片，HE染色，在生物光学显微镜下观察。

腹间隔室综合征动物模型腹腔压力-容积曲线是一条腹腔压力在腹腔容积达一定程度后随容积增加而快速升高的单向曲线，二者呈函数关系：Y=-75.13＋63.45X，二者呈正相关关系（r²=0.9407，P=0.0001）。病理改变：（1）肾：镜下肾皮髓界限明确，肾小球有集中现象，系膜细胞（＋）及基质（＋）增生，近曲小管上皮细胞颗粒、气球变性，部分腔内可见大量粉染管型，管型结构呈不规则球状，另有一些蛋白管型，近曲小管间有出血。远曲小管可见玻璃样变性。（2）肝：肝细胞广泛气球样变性，伴颗粒变性，汇管区血管显著扩张、充血，内有大量红染的液体及脂褐素沉积，伴少量炎细胞浸润。

水囊叠加加压法可制作羊腹腔高压动物模型。

To establish an improved animal model of abdominal compartment syndrome by adding water in water sac successively.

Five sheep were chosen as experimental subjects. After anesthesia, 6.0 cm incisions were made along the midline with suture. One empty water sac was placed at first, and then six basic water sacs (total volume, 1200 ml) were added. The sutures were then knotted and the abdomen was closed without tension. Animal models were made by adding normal saline into the water sac through a conduit. The pressure was measured every 50 ml of normal saline were added, and these data were used to plot a curve. The modeling process was finished once the pressure reached 45-50 cmH₂O. At 72 hours after surgery, the sheep were executed and livers and kidneys were cut out, fixed in 10% formaldehyde solution, paraffin-embedded, and sectioned. The sections were stained with hematoxylin and eosin and observed under an optical microscope.

A curve of ACS animal abdominal volume-pressure was plotted. It showed that after a certain point, the abdominal pressure increased dramatically while abdominal volume increased (Y=-75.13+ 63.45X), which had a positive correlation (r²=0.9407, P=0.0001). Pathological examination of the kidney showed that the boundary between the cortex and medulla was clear. Glomeruli were concentrated in some parts. There was proliferation of mesangial cells (+ ) and matrix (+ ). Ballooning degeneration and granular degeneration were observed in some cells. There were many powder casts in some cavities, which were present as irregular globes. Besides, some protein casts and proximal convoluted tubule hemorrhage were observed. Distal convoluted tubules had hyaline degeneration. Pathological examination of the liver showed that many liver cells had ballooning degeneration and granular degeneration. There were dilation, congestion, and a large number of red stained liquid and lipofuscin deposition in blood vessels in the portal area, with a small amount of inflammatory cell infiltration.

An improved sheep model of abdominal compartment syndrome has been successfully created by adding water in water sac successively.