晚期氧化蛋白产物对妊娠滋养细胞生物学功能的影响

doi:10.3877/cma.j.issn.1674-0785.2019.02.010

中华临床医师杂志(电子版) ›› 2019, Vol. 13 ›› Issue (02) : 129 -135. doi: 10.3877/cma.j.issn.1674-0785.2019.02.010

所属专题：文献；

基础研究

晚期氧化蛋白产物对妊娠滋养细胞生物学功能的影响

王硕石¹, 王英兰¹, 张竣², 折瑞莲¹, 钟梅³^,^†(

)

^1. 518002　暨南大学第二临床医学院深圳市人民医院妇产科
^2. 518002　暨南大学第二临床医学院深圳市人民医院计划生育科
^3. 510515　广州，南方医科大学南方医院妇产科

收稿日期:2018-11-07 出版日期:2019-01-15
通信作者: 钟梅
基金资助:
国家自然科学基金(81671466); 广东省自然科学基金项目(2015A030313003); 广东省自然科学基金项目(2018A0303100021); 深圳市卫生计生系统科研项目(201505003); 深圳市科技创新委员会知识创新计划(JCYJ20150403101028171)

Modulatory effect of advanced oxidation protein products on biological function of human first trimester trophoblast cells

Shuoshi Wang¹, Yinglan Wang¹, Jun Zhang², Ruilian Zhe¹, Mei Zhong³^,^†()

^1. Department of Obstetrics and Gynecology, 2nd Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen 518002, China
^2. Department of Family Planning, 2nd Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen 518002, China
^3. Department of Obstetrics and Gynecology, Southern Medical University, Nanfang Hospital, Guangzhou 510515, China

Received:2018-11-07 Published:2019-01-15
Corresponding author: Mei Zhong
About author:
Corresponding author: Zhong Mei, Email: zhongmei@fimmu.com

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引用本文：

王硕石, 王英兰, 张竣, 折瑞莲, 钟梅. 晚期氧化蛋白产物对妊娠滋养细胞生物学功能的影响[J]. 中华临床医师杂志(电子版), 2019, 13(02): 129-135.

Shuoshi Wang, Yinglan Wang, Jun Zhang, Ruilian Zhe, Mei Zhong. Modulatory effect of advanced oxidation protein products on biological function of human first trimester trophoblast cells[J]. Chinese Journal of Clinicians(Electronic Edition), 2019, 13(02): 129-135.

目的

观察晚期氧化蛋白产物（AOPP）对妊娠滋养细胞（HTR8/SVneo）功能的影响。

方法

体外培养HTR8/SVneo细胞株，用次氯酸（HClO）氧化鼠白蛋白（MSA）体外制备AOPP-MSA，将HTR8/SVneo滋养细胞与不同浓度（50 μg/ml组、100 μg/ml组或200 μg/ml组）的AOPP-MSA共同培养。四甲基偶氮唑蓝（MTT）比色法检测滋养细胞的增殖能力，以平均吸光度（A）值表示；侵袭小室检测滋养细胞侵袭能力，以穿膜细胞数表示；检测上清液中滋养细胞分泌的β-HCG水平评价滋养细胞分化能力；Hoechest33258染色检测滋养细胞凋亡。采用单因素方差分析比较不同浓度AOPP下，滋养细胞的增殖能力、侵袭能力、细胞分泌β-HCG、细胞凋亡数的差异。

结果

与对照组比较，AOPP浓度为50 μg/ml时，对HTR8SVneo滋养细胞增殖、侵袭、分化能力及凋亡影响不明显（P>0.05）；AOPP浓度为100 μg/ml和200 μg/ml时，AOPP明显抑制滋养细胞增殖、侵袭和分化能力，并促进滋养细胞凋亡（P<0.05）。

结论

AOPP可以抑制滋养细胞的增殖、侵袭、分化能力及诱导滋养细胞凋亡。降低血浆中AOPP水平可能有利于改善滋养细胞生物学功能。

关键词: 晚期氧化蛋白产物, 妊娠滋养细胞, 增殖能力, 侵袭能力, 分化能力

Objective

To evaluate the effects of advanced oxidation protein products (AOPP) on the biological function of first-trimester trophoblast cell line (HTR8/SVneo).

Methods

HTR8/SVneo cells were cultured in vitro. Mouse serum albumin (MAS) was oxidized by hypochloric acid (HClO) to prepare AOPP-MAS in vitro. Based on concentration of AOPP-MAS used, the HTR8/SVneo cells were classified into a 50 μg/mL group, 100 μg/mL group, and 200 μg/ml group. Methyl thiazolyl tetrazolium (MTT) assay was used to assess the cell proliferation, which was showed by the mean absorbance (A) value. Transwell assay was used to measure cell invasion, which was showed by the number of cells passing the filter membrane. β-HCG secretion was used to assess the cell differentiation. The apoptosis of trophoblast cells was detected by Hoechest33258 staining. One-way ANOVA was used to compare the proliferation, invasive ability, β-HCG, and apoptosis of trophoblast cells treated with different concentrations of AOPP.

Results

Compared with the control group, the 50 μg/ml AOPP group had no significant change in the proliferation, invasion, differentiation, or apoptosis of HTR8SVneo cells (P>0.05). However, when AOPP concentration was 100 μg/ml or 200 μg/ml, AOPP significantly inhibited the proliferation, invasion, and differentiation of trophoblast cells, and increased the apoptosis of trophoblast cells (P<0.05).

Conclusion

AOPP could modulate trophoblast cell proliferation, invasion, and differentiation and induce apoptosis of trophoblast cells. Down-regulation of plasma AOPP levels may help to improve the biological function of trophoblast cells.

Key words: Advanced oxidation protein products, Trophoblast cells, Proliferation, Invasion, Differentiation

图1 妊娠滋养细胞增殖能力的检测结果

图2 不同浓度的AOPP抑制妊娠滋养细胞的能力（苏木精染色，×400）

图3 AOPP抑制妊娠滋养细胞分泌β-HCG的能力

图4 不同浓度的AOPP诱导妊娠滋养细胞的凋亡（Hochest33258染色，×400）

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