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中华临床医师杂志(电子版) ›› 2019, Vol. 13 ›› Issue (09) : 680 -683. doi: 10.3877/cma.j.issn.1674-0785.2019.09.008

所属专题: 文献

综述

纯化痘病毒筛选的常用方法
方敬敬1, 唐慧2,()   
  1. 1. 650504 昆明理工大学医学院;650032 云南省第一人民医院,昆明理工大学附属医院,临床基础医学研究所,云南省临床病毒学重点实验室,昆明市肿瘤分子与免疫防治重点实验室
    2. 650032 云南省第一人民医院,昆明理工大学附属医院,临床基础医学研究所,云南省临床病毒学重点实验室,昆明市肿瘤分子与免疫防治重点实验室
  • 收稿日期:2019-03-06 出版日期:2019-05-01
  • 通信作者: 唐慧
  • 基金资助:
    国家自然科学基金项目(81460463); 云南省中青年学术技术带头人后备人才培养基金(2013HB083); 云南省卫生和计划生育委员会医学学科带头人培养基金(D-201642)

Commonly used methods for screening and purifying vaccinia virus

Jingjing Fang1, Hui Tang2,()   

  1. 1. College of Medicine, Kunming University of Science and Technology, Kunming 650504, China; Institute of Basic Medical Sciences, Affiliated Hospital of Kunming University of Science and Technology, The First People′s Hospital of Yunnan Province, Yunnan Provincial Key Laboratory of Clinical Virology, Kunming Key Laboratory of Tumor Molecular & Immune Prevention, Kunming 650032, China
    2. Institute of Basic Medical Sciences, Affiliated Hospital of Kunming University of Science and Technology, The First People′s Hospital of Yunnan Province, Yunnan Provincial Key Laboratory of Clinical Virology, Kunming Key Laboratory of Tumor Molecular & Immune Prevention, Kunming 650032, China
  • Received:2019-03-06 Published:2019-05-01
  • Corresponding author: Hui Tang
  • About author:
    Corresponding author: Tang Hui, Email:
引用本文:

方敬敬, 唐慧. 纯化痘病毒筛选的常用方法[J]. 中华临床医师杂志(电子版), 2019, 13(09): 680-683.

Jingjing Fang, Hui Tang. Commonly used methods for screening and purifying vaccinia virus[J]. Chinese Journal of Clinicians(Electronic Edition), 2019, 13(09): 680-683.

痘病毒是自然界普遍存在的DNA病毒,能够高效表达外源基因,诱导较强的细胞免疫和体液免疫,受到基因治疗学者的广泛关注。痘病毒发生同源重组的概率较低,所以有效地筛选和纯化痘病毒十分重要。目前常用的筛选重组痘病毒方法有基于CRISPR进行筛选;根据报告基因GFP、LacZ、GPT等进行筛选;利用TK-和Brdu结合荧光进行筛选;利用药物抗性基因与荧光或显色基因融合,即可在药物筛选的同时观察荧光或显色基因表达情况。本文旨在对目前常用的痘病毒的筛选纯化方法进行综述。

关键词: 痘病毒, 筛选

Vaccinia virus is a ubiquitous DNA virus in nature. It can express exogenous genes efficiently and induce both humoral and cellular immunity. This property has made it become a hot topic in gene therapy research. Since the probability of homologous recombination is low, effective screening and purification of vaccinia virus is very important. At present, the commonly used methods for screening vaccinia virus are based on the use of reporter genes, such as GFP, LacZ, gpt, and other genes, or the use of TK- and Brdu combined with fluorescence. Some scholars have tried to fuse drug resistance gene, fluorescence, and chromogenic genes to carry out drug screening and observe gene expression. In this article, we will make a summary of the commonly used methods for vaccinia virus screening and purification, and compare their advantages and disadvantages.

表1 重组痘病毒经常使用的报告基因
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