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中华临床医师杂志(电子版)

中华临床医师杂志(电子版) ›› 2022, Vol. 16 ›› Issue (12) : 1175 -1179. doi: 10.3877/cma.j.issn.1674-0785.2022.12.005

全科医学·临床研究

中老年硒及硒蛋白水平与血脂的关联性研究
郑家阳1, 林锦钰2, 王张民2, 罗琴2, 张琳1, 秦立强1, 陈婧司1,†(), 尹雪斌2   
  1. 1. 215123　江苏苏州，苏州大学公共卫生学院
    2. 215123　江苏苏州，中科大苏州高等研究院功能农业重点实验室；210008　江苏南京，南京恒宝田功能农业产业研究院
  • 收稿日期:2022-04-20 出版日期:2022-12-15
  • 通信作者: 陈婧司
  • 基金资助:
    膳食硒摄入安全范围研究（国际合作）(D20180031); 南京国家农创园功能农业发展专项资金(NJGJNCY-FAST01); 天然生物营养强化国际合作计划（NBP）宜春研究基地建设服务项目(大业-YC2019-121)

Association of selenium and selenoprotein with blood lipids in elderly people

Jiayang Zheng1, Jinyu Lin2, Zhangmin Wang2, Qin Luo2, Lin Zhang1, LiQiang Qin1, Jingsi Chen1,†(), Xuebin Yin2   

  1. 1. School of Public Health, Soochow University, Suzhou 215123, China
    2. Key Laboratory of Functional Agriculture, Suzhou Institute for Advanced Study, USTCM, Suzhou 215123, China; Nanjing Hengbaotian Functional Agricultural Industry Research Institute, Nanjing 210008, China
  • Received:2022-04-20 Published:2022-12-15
  • Corresponding author: Jingsi Chen
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引用本文：

郑家阳, 林锦钰, 王张民, 罗琴, 张琳, 秦立强, 陈婧司, 尹雪斌. 中老年硒及硒蛋白水平与血脂的关联性研究[J]. 中华临床医师杂志(电子版), 2022, 16(12): 1175-1179.

Jiayang Zheng, Jinyu Lin, Zhangmin Wang, Qin Luo, Lin Zhang, LiQiang Qin, Jingsi Chen, Xuebin Yin. Association of selenium and selenoprotein with blood lipids in elderly people[J]. Chinese Journal of Clinicians(Electronic Edition), 2022, 16(12): 1175-1179.

目的

探讨中老年硒水平与血脂指标的相关性，及抗氧化酶超氧化物歧化酶（superoxide dismutase，SOD）在两者关联的中介作用。

方法

对宜春市的85名中老年居民进行横断面观察，收集其血样，对血浆中硒、硒蛋白［硒蛋白P（selenoprotein P，SEPP1）和谷胱甘肽过氧化物酶（glutathione peroxidase，GPX）］及血脂浓度进行检测，采用线性回归分析硒水平与SOD、血脂指标的相关性，筛选出与SOD和血脂指标相关联的硒生物标志，进行中介效应分析。

结果

线性回归结果显示血浆硒浓度与4个血脂指标均无显著相关性，但硒蛋白（SEPP1和GPX）与血脂指标低密度蛋白胆固醇（low density protein cholesterol，LDL-C）和总胆固醇（total cholesterol，TC）均呈显著负相关，且与抗氧化酶SOD正相关（P＜0.05）。中介效应分析结果显示SOD在SEPP1与LDL-C关联中具有显著中介作用（P＜0.05）。

结论

硒蛋白可能通过抑制氧化损伤进而降低血脂水平。

关键词: 硒, 硒蛋白P, 超氧化物歧化酶, 中介效应
Objective

To investigate the association between the levels of selenium and blood lipids in middle-aged and elderly people, and assess the mediatory effect of superoxide dismutase (SOD) in their association.

Methods

A cross-sectional study was conducted in 85 middle-aged and elderly people in Yichun. Blood samples were collected to determine the concentrations of selenium, selenoproteins [selenoprotein P (SEPP1) and glutathione peroxidase (GPX)], and blood lipids. Linear regression analyses were performed to investigate the association between the levels of selenium and SOD, as well as the association between the levels of selenium and blood lipids. Then,selenium biomarkers related to blood lipids were identified to further investigate whether there is any mediatory effect in the relationship between selenium biomakers and blood lipids.

Results

Linear regression analysis revealed that the level of selenium in plasma was not correlated with blood lipids, but the selenoproteins (SEPP1 and GPX) were negatively associated with low density protein cholesterol (LDL-C) and total cholesterol (TC). In addition, selenoproteins were found to be associated with SOD (P＜0.05). In the mediation analysis, SOD was found to be a significant mediator of the association between SEPP1 and LDL-C (P＜0.05).

Conclusion

Selenoproteins may reduce blood lipid levels by inhibiting oxidative stress.

Key words: Selenium, Selenoprotein P, Superoxide dismutase, Mediatory effect
表1 研究对象基本情况
指标 类别 分布描述
年龄［median（IQR），year］ - 56（51，65）
性别（n，%） 男 38（44.7）
女 47（55.3）
吸烟情况（n，%） 从不 61（71.8）
现在/曾经吸烟 24（28.2）
饮酒情况（n，%） 从不 65（76.5）
现在/曾经饮酒 20（23.5）
BMI［median（IQR），kg/m2］ - 21.8（20.1，24.2）
收缩压［median（IQR），mm Hg］ - 130（120，140）
舒张压［median（IQR），mm Hg］ - 80（75，90）
血浆硒［median（IQR），μg/L］ - 130.1（93.5，154.7）
SEPP1［median（IQR），μg/mL］ - 24.9（17.8，34.1）
GPX［median（IQR），U/L］ - 122.3（94.9，182.5）
SOD［median（IQR），U/mL］ - 48.7（28.2，93.6）
LDL-C［median（IQR），mmol/L］ - 2.0（2.6，3.6）
HDL-C［median（IQR），mmol/L］ - 1.1（0.8，1.4）
TC［median（IQR），mmol/L］ - 4.7（4.2，5.4）
TG［median（IQR），mmol/L］ - 1.1（0.8，1.9）
表2 血浆硒和硒蛋白水平与血脂指标的多元线性回归
LDL-C HDL-C TC TG
β P值 β P值 β P值 β P值
Model 1
血浆硒 0.088 0.425 -0.060 0.588 0.176 0.107 0.250 0.021
SEPP1 -0.212 0.046 0.063 0.564 -0.220 0.043 -0.107 0.332
GPX -0.241 0.026 -0.081 0.463 -0.296 0.006 -0.056 0.611
Model 2
血浆硒 0.105 0.382 -0.001 0.991 0.198 0.096 0.203 0.086
SEPP1 -0.293 0.047 0.077 0.486 -0.213 0.051 -0.124 0.258
GPX -0.242 0.027 -0.063 0.568 -0.301 0.005 -0.082 0.453
Model 3
血浆硒 0.101 0.406 0.001 0.997 0.194 0.108 0.200 0.089
SEPP1 -0.221 0.047 0.080 0.467 -0.227 0.039 -0.130 0.230
GPX -0.247 0.025 -0.062 0.574 -0.306 0.005 -0.085 0.435
图1 血浆硒和硒蛋白水平与SOD的线性回归分析
表3 SOD在SEPP1与LDL-C关联中的中介效应
效应 β SE P值 Lower 95%CI Upper 95%CI
A（暴露-中介变量） 3.325 0.252 ＜0.001 2.825 3.826
B（中介变量-结局） -0.005 0.002 0.031 -0.010 -0.001
C（直接效应） 0.006 0.009 0.511 -0.012 0.025
Ab（中介效应） -0.017 0.007 0.015 -0.032 -0.004
图2 中介效应
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