分析张家口地区碳青霉烯耐药铜绿假单胞菌（CRPA）的耐药机制。

收集2021年6月至2022年7月河北北方学院附属第一医院临床分离的49株非重复碳青霉烯耐药铜绿假单胞菌菌株进行菌株鉴定与药敏分析，提取CRPA菌株DNA，采用二代测序技术测序，分析其耐药机制。

49株CRPA对不同抗菌药物的耐药率分别为：亚胺培南100%，美罗培南75.51%，氨曲南63.27%，左氧氟沙星57.14%，头孢他啶55.10%，美罗培南法硼巴坦55.10%，头孢吡肟55.10%，头孢哌酮舒巴坦53.06%，哌拉西林他唑巴坦46.94%，环丙沙星46.94%，头孢他啶阿维巴坦46.94%，亚胺培南瑞丽巴坦36.73%，阿米卡星34.68%，头孢洛扎他唑巴坦34.69%，磷霉素28.57%，多黏菌素B 6.12%。49株CRPA中仅3株检出携带碳青霉烯酶基因，14株携带OXA-10型β-内酰胺酶基因。

产碳青霉烯酶非张家口地区CRPA对碳青霉烯类药物耐药的主要耐药机制，其具体机制仍需进一步试验探究。

To analyze the antimicrobial drug resistance of carbapenem-resistant Pseudomonas aeruginosa (CRPA) and the possible mechanisms in Zhangjiakou, China.

A total of 49 non-repetitive CRPA isolates from clinical samples were collected in Zhangjiakou, China between June 2021 and July 2022 for strain identification and antimicrobial susceptibility testing. DNA was extracted from CRPA isolates, and next-generation sequencing technology was used to sequence their genomes to explore their resistance mechanisms.

The resistance rates of 49 strains of CRPA to different antibacterial drugs were as follows: imipenem 100%, meropenem 75.51%, amikacin 63.27%, levofloxacin 57.14%, ceftazidime 55.10%, polymyxin B 55.10%, cefepime 55.10%, ceftolozane-tazobactam 53.06%, piperacillin-tazobactam 46.94%, ciprofloxacin 46.94%, ceftazidime-avibactam 46.94%, imipenem-relebactam 36.73%, amoxicillin 34.68%, ceftolozane-tazobactam 34.69%, colistin 28.57%, and polymyxin B 6.12%. Among the 49 CRPA strains, only 3 were found to carry carbapenemase genes, and 14 carried the OXA-10 type β-lactamase gene.

Carbapenemase production is the main resistance mechanism of CRPA to carbapenems in areas other than Zhangjiakou. The specific mechanism still requires further experimental investigation.