Role of lincRNA ULK4P2 in biological behavior of hepatocellular carcinoma cells

doi:10.3877/cma.j.issn.1674-0785.2018.08.006

Abstract

Abstract:

Objective

To investigate the role of the long-strand intergenic non-coding RNA (lincRNA) ULK4P2 in the biological behavior of hepatocellular carcinoma cells by interfering with the expression of lincRNA ULK4P2 in hepatocellular carcinoma cell lines.

Methods

Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of lincRNA ULK4P2 in different hepatoma cell lines and normal cells. According to the results of RT-qPCR, hepatocellular carcinoma cell line HepG2 was selected for subsequent interference experiments. Small interfering RNA (siRNA) targeting lincRNA ULK4P2, including siRNA-1, siRNA-2, and siRNA-3, as well as siRNA-mock (blank control) and siRNA-Scramble (reconstructed after shuffling the siRNA sequence of interest; negative control) were transfected into HepG2 cells, and siRNA-2 was selected for subsequent experiments according to the interference effect on lincRNA ULK4P2. The expression of lincRNA ULK4P2 in HepG2 cells was down-regulated by siRNA-mediated interference. The role of lincRNA ULK4P2 expression in the proliferation of hepatoma cells was studied by CCK-8 assay. Flow cytometry was used to detect the expression of lincRNA ULK4P2 and the influence on apoptosis and cell cycle distribution of liver cancer cells. One-way analysis of variance was used to compare the proliferation activity (absorbance) and cell cycle distribution of HepG2 cells transfected with siRNA-2, siRNA-mock, or siRNA-Scramble.

Results

Compared with the siRNA-mock group and siRNA-Scramble group, the proliferation of hepatocellular carcinoma cells was significantly inhibited after the down-regulation of lincRNA ULK4P2 in the siRNA-2 group (P<0.05). After down-regulating the expression of lincRNA ULK4P2 in HepG2 cells, there was no significant change in the number of cells in each phase. The proportion of G2/M phase cells in the siRNA-2 group (43.92%) was significantly higher than that in the siRNA-mock group (7.81%) and siRNA-Scramble group (8.21%) (P<0.05).

Conclusion

Our findings indicate that lincRNA ULK4P2 may be involved in the proliferation of hepatocellular carcinoma cells .

Key words: Long intergenic non-coding RNA ULK4P2, Hepatocellular carcinoma, Mechanism

Tingting Yu, Fei Chen, Hongshan Yang, Jun Li. Role of lincRNA ULK4P2 in biological behavior of hepatocellular carcinoma cells[J]. Chinese Journal of Clinicians(Electronic Edition), 2018, 12(08): 456-461.

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Figures/Tables 4

References 15

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