Clinical significance of expression of glutathione peroxidase 2 in lung adenocarcinoma

doi:10.3877/cma.j.issn.1674-0785.2020.06.001

Abstract

Abstract:

Objective

To explore the significance of expression of glutathione peroxidase 2 (GPX2) in lung adenocarcinoma (LUAD).

Methods

One hundred and fifty-two cases of primary LUAD and matched paracancerous tissue samples were collected from January 2010 to December 2013 at the Department of Pathology of Yijishan Hospital of Wannan Medical College and the Department of Pathology of Xuzhou Central Hospital. Immunohistochemistry was used to detect the protein expression of GPX2 and ATP binding cassette subfamily B member 6 (ABCB6). Positron emission tomography/computed tomography (PET/CT) was performed preoperatively. The expression of GPX2 mRNA in LUAD tissues was analyzed online with GEPIA and cBioPortal tools. The t-test was used to compare the difference between GPX2 expression and maximum standardized uptake value (SUV) of 18-fluorodeoxyglucose (¹⁸F-FDG) in lung cancer tissues and normal tissues, Pearson correlation analysis was performed to analyze the correlation between GPX2 expression and ABCB6 protein expression, the χ²-test was used to compare the difference between different clinicopathological features, and Kaplan-Meier method was used to analyze the survival prognosis of lung adenocarcinoma patients.

Results

Based on the TCGA and GTEx data, GPX2 mRNA expression was upregulated in LUAD tissues (n=483) compared with that in normal tissues (n=347), and it was positively correlated with ABCB6 mRNA expression (r=0.57, P<0.001). In our cohort, there were 89 (58.6%) cases showing a higher level of GPX2 protein expression in tumor tissues than in adjacent normal tissues (P<0.001). Also, GPX2 protein expression was positively correlated with ABCB6 expression (r=0.697, P<0.001). Furthermore, the maximum SUV of ¹⁸F-FDG was higher in the GPX2 high expression group (n=29) than in the GPX2 low expression group (n=26). Although there were no correlations between GPX2 expression and patients′ clinicopathological features, LUAD patients with elevated GPX2 expression had a shorter disease-free survival (DFS) than those with low GPX2 expression [mean 34.75 (95%CI: 29.746-39.747) months vs 48.91 (95%CI: 41.033-56.779) months, P=0.009].

Conclusion

GPX2 acts as an oncogene in LUAD and promotes LUAD progression by regulating ABCB6-mediated glycolysis.

Key words: Adenocarcinoma, lung, Glutathione peroxidase, ATP binding cassette subfamily B member 6, Positron emission tomography/computed tomography, Prognosis

Nanlin Jiao, Bi Chen, Youwei Zhang, Sanyuan Sun. Clinical significance of expression of glutathione peroxidase 2 in lung adenocarcinoma[J]. Chinese Journal of Clinicians(Electronic Edition), 2020, 14(06): 401-406.

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Figures/Tables 4

References 25

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病理因素		例数	高/中表达	低表达	χ²值	P值
性别		?	?	?	0.547	0.460
?	男	85	52	33	?	?
?	女	67	37	30	?	?
年龄（岁）		?	?	?	0.269	0.604
?	<60	71	40	31	?	?
?	≥60	81	49	32	?	?
肿瘤大小（cm）		?	?	?	0.344	0.557
?	>3	67	41	26	?	?
?	≤3	85	48	37	?	?
分化程度		?	?	?	1.703	0.192
?	好/中	102	56	46	?	?
?	差	50	33	17	?	?
淋巴结转移		?	?	?	1.280	0.258
?	无	81	44	37	?	?
?	有	71	45	26	?	?
TNM分期		?	?	?	1.596	0.206
?	Ⅰ/Ⅱ	68	36	32	?	?
?	Ⅲ/Ⅳ	84	53	31	?	?

病理因素		例数	高/中表达	低表达	χ²值	P值
性别		?	?	?	0.547	0.460
?	男	85	52	33	?	?
?	女	67	37	30	?	?
年龄（岁）		?	?	?	0.269	0.604
?	<60	71	40	31	?	?
?	≥60	81	49	32	?	?
肿瘤大小（cm）		?	?	?	0.344	0.557
?	>3	67	41	26	?	?
?	≤3	85	48	37	?	?
分化程度		?	?	?	1.703	0.192
?	好/中	102	56	46	?	?
?	差	50	33	17	?	?
淋巴结转移		?	?	?	1.280	0.258
?	无	81	44	37	?	?
?	有	71	45	26	?	?
TNM分期		?	?	?	1.596	0.206
?	Ⅰ/Ⅱ	68	36	32	?	?
?	Ⅲ/Ⅳ	84	53	31	?	?

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