To analyze the different biological phenotypic characteristics of Klebsiella pneumoniae and provide an experimental basis for clinical anti-infection treatment.

Clinical Klebsiella pneumoniae isolates were collected from the Qingdao Municipal Hospital, China from January 2018 to October 2019. VITEK-2 compact automatic bacterial identification/susceptibility sensitivity system was used for identification and drug sensitivity testing of clinically isolated strains. The origin and distribution of Klebsiella pneumoniae specimens were analyzed, and the differences in drug resistance rates of Klebsiella pneumoniae with different biological phenotypes were compared.

A total of 1435 strains of Klebsiella pneumoniae were isolated, among which 316 (22.0%) produced extended-spectrum beta-lactamases (ESBLs), 177 (12.3%) were carbapenem-resistant (CR-KP), and 113 (7.9%) were high-virulent (HvKP). Sputum specimens were the most abundant source, in which 489 strains were detected, accounting for 34.1%; the second was urine specimens, with 220 strains isolated, accounting for 15.3%. The departments involved were mainly intensive care unit, emergency ward, and neurosugical ward, accounting for 21.8%, 15.4%, and 10.8%, respectively. Drug sensitivity results showed that the sensitivity of Klebsiella pneumoniae with different biological phenotypes to commonly used antibiotics was quite different; CR-KP had the highest resistance, with a drug resistance rate to most antibiotics being above 80%, followed by ESBLs, and HvKP strains had the lowest resistance, with a drug resistance rate to most antibiotics being less than 10%.

Klebsiella pneumoniae causing nosocomial infection is widely distributed clinically, which can be manifested as a variety of biological phenotypes, and its sensitivity to commonly used antibacterial drugs is also quite different. Therefore, clinical attention should be paid to pathogen examination in the diagnosis and treatment of infectious diseases.