Relationship of peripheral blood exosomes and RhoA/ROCK signaling pathway with bleeding symptoms in systemic lupus erythematosus patients with immune thrombocytopenia

doi:10.3877/cma.j.issn.1674-0785.2021.03.008

Abstract

Abstract:

Objective

To investigate the expression of plasma exosomes and the activation of the RhoA/ROCK signaling pathway in peripheral blood of systemic lupus erythematosus (SLE) patients with immune thrombocytopenia with or without bleeding symptoms, and to explore the related mechanism of bleeding symptoms.

Methods

The data of 22 SLE patients with immune thrombocytopenia diagnosed at the Department of Rheumatology and Clinical Immunology, the Affiliated Hospital of Qingdao University from January 2018 to January 2020 were collected. The patients were divided into two groups according to whether bleeding symptoms were present. Peripheral blood was collected and plasma exosomes were separated. The exosomes were observed by transmission electron microscopy, and the exosomes markers CD63 and CD81 were detected by Western blot. Pull-down method was used to detect the activation of RhoA/ROCK signaling pathway in mononuclear cells (MNC), and RT-PCR was used to detect the mRNA expression of RhoA/ROCK. The correlation between RhoA/ROCK mRNA expression and clinical indicators was analyzed.

Results

The number of plasma exosomes was decreased in patients with bleeding symptoms compared to that of the patients without bleeding symptoms. The activation of RhoA/ROCK signal pathway as well as the mRNA expression of RhoA/ROCK was significantly elevated in patients with bleeding symptoms compared to those without (P＜0.05). The expression of RhoA/ROCK mRNA was negatively correlated with platelet count (RhoA: r=-0.333, P=0.032; ROCK: r=-0.475, P=0.046) and positively correlated with SLEDAI score (RhoA: r=0.435, P=0.042; ROCK: r=0.462, P=0.047).

Conclusion

Plasma exosomes and the RhoA/ROCK signaling pathway in peripheral blood MNC may participate in the regulation of bleeding symptoms in SLE patients with immune thrombocytopenia.

Key words: Systemic lupus erythematosus, Thrombocytopenia, Exosomes, RhoA/ROCK signaling pathway

Hongyan Feng, Xuexia Sun, Lei Zhao, Liqin Wang, Yuanyuan Zhang, Jing Dong. Relationship of peripheral blood exosomes and RhoA/ROCK signaling pathway with bleeding symptoms in systemic lupus erythematosus patients with immune thrombocytopenia[J]. Chinese Journal of Clinicians(Electronic Edition), 2021, 15(03): 194-199.

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References 26

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