Value of quantitative analysis of DNA ploidy in diagnosis of lung cancer

doi:10.3877/cma.j.issn.1674-0785.2021.05.003

Abstract

Abstract:

Objective

To explore the value of DNA image cytometry (DNA-ICM) in lung cancer diagnosis.

Methods

A total of 102 specimens were obtained from Department of Pathology, Beijing Chest Hospital, Capital Medical University from September to October 2019,including 48 cases of sputum, 31 cases of thoracic-peritoneal effusion, and 23 cases of bronchial lavage fluid. Two slides were prepared from each case: one was stained with hematoxylin and eosin for conventional liquid-based cytology (LBC) examination, and the other was stained by Feulgen method for determination of DNA amount using automated DNA-ICM method. Diagnostic value of DNA-ICM and LBC in lung cancer was evaluated using the results of pathological biopsy as the golden standard.

Results

(1) The sensitivity and specificity of DNA-ICM in diagnosing lung cancer were 73.4％and 69.6％, and those of LBC were 54.4％and 82.6％, respectively. The sensitivity of DNA-ICM was significantly higher than that of LBC (P＜0.05). Combining the above two methods, the sensitivity and specificity were 77.2% and 69.6%, respectively. (2) As for the specimen type, the sensitivity of DNA-ICM in alveolar lavage fluid was higher that in sputum (P＜0.05). (3) The sensitivity of DNA-ICM in adenocarcinoma was higher than that in small cell carcinoma among different histopathological classifications of lung cancer (P＜0.05).

Conclusion

DNA-ICM is valuable in diagnosing lung cancer. Combining of LBC and DNA-ICM can further improve the detection rate of tumors. This combined method is promising for early screening and diagnosing of lung cancer.

Key words: DNA image cytometry, Liquid-based cytology, Lung cancer

Xuejing Chen, Lijuan Zhou, Weili Du, Kun Li, Nanying Che. Value of quantitative analysis of DNA ploidy in diagnosis of lung cancer[J]. Chinese Journal of Clinicians(Electronic Edition), 2021, 15(05): 331-334.

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URL: https://zhlcyszz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0785.2021.05.003

https://zhlcyszz.cma-cmc.com.cn/EN/Y2021/V15/I05/331

Figures/Tables 2

References 11

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方法	肺癌（例）		非癌（例）		敏感度（%）	特异度（%）	符合率（%）	漏诊率（%）
方法	阳性	阴性	阳性	阴性	敏感度（%）	特异度（%）	符合率（%）	漏诊率（%）
DNA-ICM	58	21	7	16	73.4^a	69.6	72.5	26.6
LBC	43	36	4	19	54.4	82.6	60.8	46.6
联合应用	61	18	7	16	77.2	69.6	75.5	22.8

方法	肺癌（例）		非癌（例）		敏感度（%）	特异度（%）	符合率（%）	漏诊率（%）
方法	阳性	阴性	阳性	阴性	敏感度（%）	特异度（%）	符合率（%）	漏诊率（%）
DNA-ICM	58	21	7	16	73.4^a	69.6	72.5	26.6
LBC	43	36	4	19	54.4	82.6	60.8	46.6
联合应用	61	18	7	16	77.2	69.6	75.5	22.8

来源	样本量（例）	DNA-ICM			LBC
来源	样本量（例）	阳性（例）	敏感度（%）	特异度（%）	阳性（例）	敏感度（%）	特异度（%）
痰	48	26	59.1	100.0	17	38.6	100.0
胸腹水	31	14	87.5	68.2	13	81.3	78.9
支气管灌洗液	23	18	94.7^a	100.0	13	68.4	100.0

来源	样本量（例）	DNA-ICM			LBC
来源	样本量（例）	阳性（例）	敏感度（%）	特异度（%）	阳性（例）	敏感度（%）	特异度（%）
痰	48	26	59.1	100.0	17	38.6	100.0
胸腹水	31	14	87.5	68.2	13	81.3	78.9
支气管灌洗液	23	18	94.7^a	100.0	13	68.4	100.0

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