Association of miR-146a and miR-499 polymorphisms with susceptibility to rheumatoid arthritis: a Meta-analysis

doi:10.3877/cma.j.issn.1674-0785.2021.06.004

Abstract

Abstract:

Objective

To evaluate the association between microRNA gene polymorphisms and rheumatoid arthritis (RA) using Meta-analysis.

Methods

A systematic search of Chinese biomedical literature database, VIP database, Wanfang data, CNKI, PubMed, Cochrane, and Embase database was performed to identify articles reporting on the association between microRNA gene polymorphisms and RA. The search time limit was from the inception of the database to May 1, 2020. The references of retrieved articles were manually searched to ensure the comprehensiveness of the retrieval. Studies meeting the inclusion criteria were included to conduct data extraction and quality evaluation. Two researchers independently screened the literature, extracted the data, and evaluated the risk of bias in the included studies. A Meta-analysis was performed using Stata 15.1 software.

Results

A total of 14 cohort studies were included, involving 1945 RA cases and 2405 controls. Meta-analysis results showed that the expression level of G/G genotype of miR-146a in RA patients was significantly higher than that in normal controls [OR=1.15, 95%CI (1.00, 1.33), P=0.048], other phenotypes were not significantly associated with the risk of RA [C/C: OR=0.94, 95%CI (0.83, 1.07), P=0.353; G/C: OR=0.92, 95%CI(0.82,1.03), P=0.135]. The C/T genotype of miR-499 in RA patients was significantly lower than that in normal controls [OR=0.84, 95%CI (0.72,1.00), P=0.044], there were no significant differences in other genotypes [T/T: OR=0.97, 95%CI (0.77,1.24), P=0.831; C/C: OR=1.40, 95%CI (0.96,2.05), P=0.713]. Analysis of subgroups by race showed that there was no statistically significant association between the polymorphisms of miR-146a and miR-499 and the incidence of RA in Caucasian and Asian populations (P＞0.05).

Conclusion

This study find that the G/G genotype of miR-146a and C/T genotype of miR-499 are related to genetic susceptibility to RA.

Key words: MicroRNA, Rheumatoid arthritis, Gene polymorphism, Meta-analysis

Yuelin Zhang, Jiaming Cao, Huiyuan Liu, Qiao Wang, Lingyu Fu. Association of miR-146a and miR-499 polymorphisms with susceptibility to rheumatoid arthritis: a Meta-analysis[J]. Chinese Journal of Clinicians(Electronic Edition), 2021, 15(06): 418-428.

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URL: https://zhlcyszz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0785.2021.06.004

https://zhlcyszz.cma-cmc.com.cn/EN/Y2021/V15/I06/418

Figures/Tables 12

编号	作者	年份（年）	国家	研究方法	样本量（例）		RA患者年龄（岁， $x ¯$ ±s）	RA患者性别（例，男/女）	微小RNA	结局指标
编号	作者	年份（年）	国家	研究方法	RA组	对照组	RA患者年龄（岁， $x ¯$ ±s）	RA患者性别（例，男/女）	微小RNA	结局指标
1	Fattah et al^［17］	2018	埃及	队列研究	100	100	41.7±8.5	14/86	miR-499	1、2、3
2	高登文等^［18］	2012	中国	队列研究	123	220	49±13	18/105	miR-146a	4、5、6
3	Zhang et al^［19］	2013	中国	队列研究	214	478	54.63±15.77	61/153	miR-499	1、2、3
4	Bogunia-Kubik et al^［20］	2016	波兰	队列研究	111	130	-	-	miR-146a	4、5、6
5	Ayeldeen et al^［21］	2018	埃及	队列研究	52	56	39.0±10.7	-	miR-499、 miR-146a	1、2、3、4、5、6
6	Ciccacci et al^［22］	2016	意大利	队列研究	192	298	54.1±13.2	45/147	miR-146a	4、5、6
7	Hassine et al^［23］	2017	突尼斯	队列研究	165	150	-	-	miR-146a	4、5、6
8	谢传美等^［24］	2018	中国	队列研究	600	398	41.4±5.9	134/466	miR-499、 miR-146a	1、2、3、4、5、6
9	肖茗渊等^［25］	2015	中国	队列研究	200	120	49.0±12.9	30/170	miR-499	1、2、3
10	Chatzikyriakidou et al^［26］	2010	希腊	队列研究	136	147	60.8±12.7	27/109	miR-146a	4、5、6
11	El-Shal et al^［27］	2013	埃及	队列研究	217	245	38.4±7.0	-	miR-499、 miR-146a	1、2、3、4、5、6
12	Alemán-Ávila et al^［28］	2017	墨西哥	队列研究	412	486	51.8±13.6	34/378	miR-499	1、2、3
13	Yang et al^［29］	2010	中国	队列研究	208	240	48±13	36/172	miR-499、 miR-146a	1、2、3、4、5、6
14	Zhou et al^［30］	2015	中国	队列研究	615	839	54.5±15.2	143/472	miR-146a	4、5、6

编号	作者	年份（年）	国家	研究方法	样本量（例）		RA患者年龄（岁， $x ¯$ ±s）	RA患者性别（例，男/女）	微小RNA	结局指标
编号	作者	年份（年）	国家	研究方法	RA组	对照组	RA患者年龄（岁， $x ¯$ ±s）	RA患者性别（例，男/女）	微小RNA	结局指标
1	Fattah et al^［17］	2018	埃及	队列研究	100	100	41.7±8.5	14/86	miR-499	1、2、3
2	高登文等^［18］	2012	中国	队列研究	123	220	49±13	18/105	miR-146a	4、5、6
3	Zhang et al^［19］	2013	中国	队列研究	214	478	54.63±15.77	61/153	miR-499	1、2、3
4	Bogunia-Kubik et al^［20］	2016	波兰	队列研究	111	130	-	-	miR-146a	4、5、6
5	Ayeldeen et al^［21］	2018	埃及	队列研究	52	56	39.0±10.7	-	miR-499、 miR-146a	1、2、3、4、5、6
6	Ciccacci et al^［22］	2016	意大利	队列研究	192	298	54.1±13.2	45/147	miR-146a	4、5、6
7	Hassine et al^［23］	2017	突尼斯	队列研究	165	150	-	-	miR-146a	4、5、6
8	谢传美等^［24］	2018	中国	队列研究	600	398	41.4±5.9	134/466	miR-499、 miR-146a	1、2、3、4、5、6
9	肖茗渊等^［25］	2015	中国	队列研究	200	120	49.0±12.9	30/170	miR-499	1、2、3
10	Chatzikyriakidou et al^［26］	2010	希腊	队列研究	136	147	60.8±12.7	27/109	miR-146a	4、5、6
11	El-Shal et al^［27］	2013	埃及	队列研究	217	245	38.4±7.0	-	miR-499、 miR-146a	1、2、3、4、5、6
12	Alemán-Ávila et al^［28］	2017	墨西哥	队列研究	412	486	51.8±13.6	34/378	miR-499	1、2、3
13	Yang et al^［29］	2010	中国	队列研究	208	240	48±13	36/172	miR-499、 miR-146a	1、2、3、4、5、6
14	Zhou et al^［30］	2015	中国	队列研究	615	839	54.5±15.2	143/472	miR-146a	4、5、6

作者	年份（年）	研究人群选择	组间可比性	暴露因素的测量	得分（分）
Fattah et al^［17］	2018	☆☆☆	☆☆	☆☆☆	8
高登文等^［18］	2012	☆☆☆	☆☆	☆☆☆	8
Zhang et al^［19］	2013	☆☆	☆☆	☆☆☆	7
Bogunia-Kubik et al^［20］	2016	☆☆☆	☆☆	☆☆☆	8
Ayeldeen et al^［21］	2018	☆☆☆	☆☆	☆☆	7
Ciccacci et al^［22］	2016	☆☆☆	☆☆	☆☆☆	8
Hassine et al^［23］	2017	☆☆	☆☆	☆☆☆	7
谢传美等^［24］	2018	☆☆☆	☆☆	☆☆	7
肖茗渊等^［25］	2015	☆☆☆	☆☆	☆☆☆	8
Chatzikyriakidou et al^［26］	2010	☆☆	☆☆	☆☆☆	7
El-Shal et al^［27］	2013	☆☆☆	☆☆	☆☆☆	8
Alemán-Ávila et al^［28］	2017	☆☆☆	☆☆	☆☆☆	8
Yang et al^［29］	2010	☆☆	☆☆	☆☆☆	7
Zhou et al^［30］	2015	☆☆	☆☆	☆☆☆	7

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