Effect of probiotics combined with periodontal therapy on inflammatory factors and bone metabolic factors in gingival crevicular fluid of patients with periodontitis combined type 2 diabetes

doi:10.3877/cma.j.issn.1674-0785.2021.07.007

Abstract

Abstract:

Objective

To analyze the effect of probiotics combined with periodontal therapy on inflammatory factors and bone metabolic factors in gingival crevicular fluid of patients with periodontitis combined type 2 diabetes.

Methods

One hundred and fifty patients with periodontitis accompanied by alveolar ridge absorption combined type 2 diabetes admitted to Fuyang Hospital of Anhui Medical University from July 2017 to January 2021 were randomly divided into study group and control group, with 75 cases in each group. All patients were given periodontal basic treatment, and study group was additionally treated with probiotics. The therapeutic effects, changes of inflammatory factors (IL-6, hs-CRP, and TNF-α) and bone metabolic factor (OC) in gingival crevicular fluid, and periodontal status (SBI, PD, and CAL) before and after treatment were compared between two groups.

Results

After 3 months of treatment, the total effective rate of study group was 85.33%, which was higher than that of control group (64.00%, P＜0.05). Compared with the values before treatment [study group: (5.41±0.66), (17.41±2.49), (7.59±1.13), (648.29±65.77) μg/L; control group: (5.38±0.71), (17.52±2.38), (7.61±1.14), (649.57±66.38) μg/L], the levels of IL-6, hs-CRP, TNF-α, and OC in gingival crevicular fluid decreased after 4 weeks of treatment in both groups [study group: (3.01±0.29), (8.23±0.91), (3.68±0.54), (346.32±51.59) μg/L; control group: (3.66±0.38), (12.14±1.24), (5.41±0.69), (450.35±52.67) μg/L; P all＜0.05]; the decreases in study group were lower than those of control group (P＜0.05). After 3 months of treatment, SBI and PD in both groups decreased [study group: 0.37±0.15, (3.32±0.41) mm; control group: 0.97±0.21, (3.71±0.36) mm] compared with those before treatment [study group: 3.67±0.45, (5.59±0.38) mm; control group: 3.65±0.47, (5.62±0.40) mm; P all＜0.05], and SBI and PD in study group were lower than those in control group after 3 months of treatment (P＜0.05).

Conclusion

On the basis of periodontal initial treatment, the application of probiotics in the treatment of type 2 diabetes patients combined periodontitis accompanying alveolar ridge absorption can achieve better clinical efficacy, and can effectively regulate inflammatory factors and bone metabolic factor in gingival crevicular fluid, and promote the improvement of periodontal status.

Key words: Probiotics, Type 2 diabetes, Alveolar ridge absorption, Periodontitis, Bone metabolic factor

Jianbo Xu, Dandan Wang, Xulin Peng. Effect of probiotics combined with periodontal therapy on inflammatory factors and bone metabolic factors in gingival crevicular fluid of patients with periodontitis combined type 2 diabetes[J]. Chinese Journal of Clinicians(Electronic Edition), 2021, 15(07): 515-519.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhlcyszz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0785.2021.07.007

https://zhlcyszz.cma-cmc.com.cn/EN/Y2021/V15/I07/515

Figures/Tables 4

组别	例数	性别［例（%）］		年龄（岁， $x ¯$ ±s）	病程（年， $x ¯$ ±s）		牙周疾病严重度［例（%）］
组别	例数	男	女	年龄（岁， $x ¯$ ±s）	2型糖尿病	牙周炎	中度	重度
研究组	75	46（61.33）	29（38.67）	44.31±10.25	7.51±1.84	2.37±0.65	42（56.00）	33（44.00）
对照组	75	48（64.00）	27（36.00）	44.48±10.17	7.66±1.59	2.41±0.59	39（52.00）	36（48.00）
统计值		χ²=0.114		t=0.102	t=0.534	t=0.395	χ²=0.242
P值		0.736		0.919	0.594	0.694	0.623

组别	例数	性别［例（%）］		年龄（岁， $x ¯$ ±s）	病程（年， $x ¯$ ±s）		牙周疾病严重度［例（%）］
组别	例数	男	女	年龄（岁， $x ¯$ ±s）	2型糖尿病	牙周炎	中度	重度
研究组	75	46（61.33）	29（38.67）	44.31±10.25	7.51±1.84	2.37±0.65	42（56.00）	33（44.00）
对照组	75	48（64.00）	27（36.00）	44.48±10.17	7.66±1.59	2.41±0.59	39（52.00）	36（48.00）
统计值		χ²=0.114		t=0.102	t=0.534	t=0.395	χ²=0.242
P值		0.736		0.919	0.594	0.694	0.623

组别	例数	显效	有效	无效	总有效
研究组	75	30（40.00）	34（45.33）	11（14.67）	64（85.33）
对照组	75	19（25.33）	29（38.67）	27（36.00）	48（64.00）
χ²值					9.023
P值					0.003

组别	例数	IL-6	hs-CRP	TNF-α	OC
研究组	75
治疗前		5.41±0.66	17.41±2.49	7.59±1.13	648.29±65.77
治疗4周		3.01±0.29^a	8.23±0.91^a	3.68±0.54^a	346.32±51.59^a
t值		28.831	29.988	27.037	31.285
P值		＜0.001	＜0.001	＜0.001	＜0.001
对照组	75
治疗前		5.38±0.71	17.52±2.38	7.61±1.14	649.57±66.38
治疗4周		3.66±0.38	12.14±1.24	5.41±0.69	450.35±52.67
t值		18.497	17.361	14.298	20.360
P值		＜0.001	＜0.001	＜0.001	＜0.001

组别	例数	SBI	CAL（mm）	PD（mm）
研究组	75
治疗前		3.67±0.45	4.01±0.39	5.59±0.38
治疗3个月		0.37±0.15^a	3.90±0.54	3.32±0.41^a
t值		60.249	1.430	35.167
P值		＜0.001	0.155	＜0.001
对照组	75
治疗前		3.65±0.47	3.98±0.41	5.62±0.40
治疗3个月		0.97±0.21	3.87±0.55	3.71±0.36
t值		45.086	1.389	30.737
P值		＜0.001	0.167	＜0.001

References 20

1	Gatej S, Gully N, Gibson R, et al. Probiotics and periodontitis—a literature review [J]. J Int Acad Periodontol, 2017, 19(2): 42-50.
2	肖玥, 叶丽君, 殷小佳, 等. 益生菌联合牙周基础治疗对慢性牙周炎的疗效分析 [J]. 实用口腔医学杂志, 2020, 36(6): 110-114.
3	Bustamante M, Oomah BD, Mosi-Roa Y, et al. Probiotics as an adjunct therapy for the treatment of halitosis, dental caries and periodontitis [J]. Probiotics Antimicro, 2020, 12(2): 325-334.
4	李永强, 张英, 周芳, 等. 益生菌含片联合基础牙周治疗对慢性牙周炎伴口臭患者口腔微生态的影响探讨 [J]. 实用临床医药杂志, 2019, 23(1): 69-72, 76.
5	Armitage GC. Development of a classification system for periodontal diseases and conditions [J]. Northwest Dent, 2000, 79(6): 31-35.
6	Copeland KC, Silverstein J, Moore KR, et al. Management of newly diagnosed type 2 diabetes mellitus (T2DM) in children and adolescents [J]. Pediatrics, 2013, 131(2): 364-382.
7	瓦莱丽·克拉里修. 牙周病诊疗指南 [M]. 潘亚萍, 译. 沈阳: 辽宁科学技术出版社, 2015: 91-92.
8	Cosme-Silva L, Dal-Fabbro R, Cintra LTA, et al. Reduced bone resorption and inflammation in apical periodontitis evoked by dietary supplementation with probiotics in rats [J]. Int Endod J, 2020, 53(8): 1084-1092.
9	杨磊, 郭留云, 程志芬. 益生菌辅助治疗慢性牙周炎的疗效的meta分析 [J]. 临床口腔医学杂志, 2019, 35(12): 752-756.
10	佘林, 冯岩, 曲静, 等. 益生菌治疗牙周炎疗效的Meta分析 [J]. 实用口腔医学杂志, 2019, 35(4): 548-553.
11	Invernici MM, Salvador SL, Silva PHF, et al. Effects of Bifidobacterium probiotic on the treatment of chronic periodontitis: a randomized clinical trial [J]. J Clin Periodontol, 2018, 45(10): 1198-1210.
12	Nguyen T, Brody H, Lin GH, et al. Probiotics, including nisin‐based probiotics, improve clinical and microbial outcomes relevant to oral and systemic diseases [J]. Periodontology, 2000, 2020 ,82(1): 173-185.
13	张美龄. 益生菌联合牙周基础治疗对慢性牙周炎患者口腔致病菌的影响 [J]. 中国微生态学杂志, 2021, 33(3): 297-300.
14	吕敏敏, 于兰, 郭洁, 等. 益生菌制剂辅助治疗慢性牙周炎疗效和美学效果观察 [J]. 中国美容医学, 2020, 28(12): 113-117.
15	Alshareef A, Attia A, Almalki M, et al. Effectiveness of probiotic lozenges in periodontal management of chronic periodontitis patients: clinical and immunological study [J]. Eur J Paediatr Dent, 2020, 14(2): 281.
16	宋金科. 用益生菌含片辅助治疗慢性牙周炎的效果探究 [J]. 当代医药论丛, 2019, 17(7): 181-182.
17	Gatej SM, Marino V, Bright R, et al. Probiotic lactobacillus rhamnosus GG prevents alveolar bone loss in a mouse model of experimental periodontitis [J]. J Clin Periodontol, 2018, 45(2): 204-212.
18	Dhaliwal PK, Grover V, Malhotra R, et al. Clinical and microbiological investigation of the effects of probiotics combined with scaling and root planing in the management of chronic periodontitis: a randomized, controlled study [J]. J Int Acad Periodontol, 2017, 19(3): 101-108.
19	Kumar S, Madurantakam P. Limited evidence shows short-term benefit of probiotics when used as an adjunct to scaling and root planing in the treatment of chronic periodontitis [J]. Evid Based Dent, 2017, 18(4): 109-110.
20	Cantore S, Ballini A, De Vito D, et al. Clinical results of improvement in periodontal condition by administration of oral probiotics [J]. J Biol Reg Homeos Ag, 2018, 32(5): 1329-1334.

[1]	Jinmei He, Lixue Yin, Jing Tan, Wenjun Zhang, Rui Wang, Mei Ren, Mingjiao Liao. Evaluation of potential left ventricular systolic dysfunction in patients with type 2 diabetes mellitus using ultrasonic myocardial work technology [J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2023, 20(10): 1029-1035.
[2]	Hong Ye, Jingzhe Lyu, Liangjun Zhong. New study progress of resveratrol in the treatment of periodontitis and diabetes [J]. Chinese Journal of Stomatological Research(Electronic Edition), 2023, 17(05): 376-380.
[3]	Yanqun Xu, Ping Li, Xing Yang, Hui Xue. Lipopolysaccharide mediates interleukin-6 release from periodontal ligament cells by promoting hyaluronan receptor CD44 transfer to nuclear [J]. Chinese Journal of Stomatological Research(Electronic Edition), 2023, 17(05): 335-344.
[4]	Xiaoyin Zhang, Yang Yu. Research progress on the occurrence and development of chronic kidney disease by Porphyromonas gingivalis [J]. Chinese Journal of Stomatological Research(Electronic Edition), 2023, 17(05): 328-334.
[5]	Chen Li, Weijia Zhang, Yaping Pan. Application and experience of the relationship between periodontitis and systematic diseases: Interpretation and enlightenment of the 2022 Joint Workshop by the EFP and WONCA Europe [J]. Chinese Journal of Stomatological Research(Electronic Edition), 2023, 17(05): 322-327.
[6]	Yueqi Chen, Rui Luo, Han Xiang, Yongyan Yu, Ting Yu. The causal association between osteoporosis and periodontitis: A two-sample Mendelian randomization study [J]. Chinese Journal of Stomatological Research(Electronic Edition), 2023, 17(04): 292-298.
[7]	Jixin He, Yanni Yang, Jiwei Wang, Jianguo Li, Ming Xie. Research progress on the mechanism of intestinal flora metabolites involved in the occurrence of chronic constipation [J]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2023, 12(06): 495-499.
[8]	Na Li, Guozhen Zhu. Progress of research on the roles of gut microbiota and its metabolites in acute kidney injury [J]. Chinese Journal of Kidney Disease Investigation(Electronic Edition), 2023, 12(04): 215-219.
[9]	Zhengying Zhang, Yang Ju, Xiaoning Liu. Effect of oral metformin on postoperative recurrence of type 2 diabetes mellitus with colorectal adenoma [J]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2023, 13(06): 485-488.
[10]	Nianyu Xue, Shengmin Zhang, Lingheng Wu, Lei Sha, Lanyue Tong, Cuiqin Shen, Zhaojun Li, Lianfang Du. Effect of serum bilirubin on myocardial function in patients with type 2 diabetes before cardiac structural changes [J]. Chinese Journal of Clinicians(Electronic Edition), 2023, 17(9): 1004-1009.
[11]	Lu Tao, Nan Chu, Jie Han, Chunying Bai, Wenli Pang, Haiyuan Yu. Association of serum PECAM-1 and Sirt1 levels with carotid atherosclerosis in type 2 diabetic patients [J]. Chinese Journal of Clinicians(Electronic Edition), 2023, 17(03): 291-296.
[12]	Wei You, Zhuoqi Wei, Wenhui Chen, Zhiyong Dong, Wah Yang, Cunchuan Wang. A case report of laparoscopic narrow and long pouch Roux-en-Y gastric bypass surgery for the treatment of severe obesity [J]. Chinese Journal of Obesity and Metabolic Diseases(Electronic Edition), 2023, 09(03): 218-223.
[13]	Jinliang Li, Maojuan Zeng, Jinbao Zhong, Weiqiang He, Wenxin Lin. Effect of semaglutide on skin microcirculatory function in obese patients with type 2 diabetes mellitus [J]. Chinese Journal of Obesity and Metabolic Diseases(Electronic Edition), 2023, 09(03): 192-196.
[14]	Geng Wang, Zefeng Xia, Kaixiong Tao. Effects of bariatric surgery on non-obese type 2 diabetes mellitus [J]. Chinese Journal of Obesity and Metabolic Diseases(Electronic Edition), 2023, 09(03): 155-160.
[15]	Manna Mu, Yingqing Hu, Yuan Li, Yongjun Zhang, Xiling Hu, Beisi Lin, Dezhao Liu. Clinical observation of desloratadine combined with pregabalinin the treatment of diabetic pruritus [J]. Chinese Journal of Obesity and Metabolic Diseases(Electronic Edition), 2023, 09(02): 114-119.

Please choose a citation manager

Content to export