Expression of DUOX2 in colon cancer and its effect on malignant behavior of SW620 and HCT116 cells

doi:10.3877/cma.j.issn.1674-0785.2021.11.017

Abstract

Abstract:

Objective

To detect the expression of dual oxidase 2 (DUOX2) in colon cancer tissues and investigate its effect on the malignant biological behavior of colon cancer cells.

Methods

Real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was used to detect the expression of DUOX2 in 97 pairs of colon cancer tissues and adjacent normal tissues which were collected from October 2018 to October 2019 at the Fourth Hospital of Hebei Medical University. siRNA was used to knock down the expression of DUOX2 in SW620 and HCT116 cells. MTS assay was used to determine the cell proliferation. Transwell and scratch assays were used to determine the migration and invasion of the cells.

Results

The relative expression of DUOX2 gene in colon cancer tissues was significantly higher than that in adjacent normal tissues [0.0265 (0.0061, 0.0869) vs 0.0087 (0.0017, 0.0385), P＜0.05]. The expression of DUOX2 gene in colon cancer was correlated with gender, T stage, lymph node metastasis, and TNM stage (P＜0.05). After knockdown of DUOX2 expression in SW620 and HCT116 cells, the migration and invasion ability decreased significantly (P＜0.05), but the proliferation ability did not change significantly (P＞0.05).

Conclusion

DUOX2 is highly expressed in colon cancer tissue. DUOX2 as an oncogene promotes the migration and invasion of colon cancer cells, suggesting that DUOX2 may be a biomarker for predicting metastasis and a potential target for treatment in colon cancer patients.

Key words: Colon cancer, Dual oxidase 2, Invasion and metastasis

Xue Zhang, Miao Yu, Jing Han, Xuhua Hu, Jiayin Liu, Dan Li, Yan Liu, Li Feng, Guiying Wang. Expression of DUOX2 in colon cancer and its effect on malignant behavior of SW620 and HCT116 cells[J]. Chinese Journal of Clinicians(Electronic Edition), 2021, 15(11): 897-903.

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References 21

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项目	例数	DUOX2［例（%）］		χ²值	P值
项目	例数	低表达（48例）	高表达（49例）	χ²值	P值
年龄				0.534	0.465
≤60岁	42	19（45.23）	23（54.76）
＞60岁	55	29（52.72）	26（47.27）
性别				4.752	0.029
男	61	25（40.98）	36（59.02）
女	36	23（63.89）	13（36.11）
部位				0.048	0.827
左	65	32（49.23）	33（50.77）
右	32	15（46.88）	17（53.12）
病理类型				0.785	0.376
腺癌	82	43（52.44）	39（47.56）
非腺癌	15	6（40）	9（60）
肿瘤大小				0.011	0.917
＜5 cm	48	23（47.92）	25（52.08）
≥5 cm	49	24（48.98）	25（51.02）
浸润深度				4.194	0.041
T1+T2	7	6（85.71）	1（14.29）
T3+T4	90	41（45.56）	49（54.44）
N分期				6.395	0.011
N0	58	36（62.07）	22（37.93）
N1+2	39	14（35.90）	25（64.10）
M分期				0.003	0.957
M0	94	49（52.13）	45（47.87）
M1	3	1（33.33）	2（66.67）
TNM分期				7.284	0.007
Ⅰ+Ⅱ	56	36（64.29）	20（35.71）
Ⅲ+Ⅳ	41	15（36.59）	26（63.41）
脉管瘤栓				0.572	0.449
阴性	72	34（47.22）	38（52.78）
阳性	25	14（56.00）	11（44.00）
神经受侵				0.127	0.721
阴性	86	44（51.16）	42（48.84）
阳性	11	5（45.45）	6（54.55）
分化程度				0.335	0.563
中、高分化	83	41（49.40）	42（50.60）
低分化	12	7（58.33）	5（41.67）

项目	例数	DUOX2［例（%）］		χ²值	P值
项目	例数	低表达（48例）	高表达（49例）	χ²值	P值
年龄				0.534	0.465
≤60岁	42	19（45.23）	23（54.76）
＞60岁	55	29（52.72）	26（47.27）
性别				4.752	0.029
男	61	25（40.98）	36（59.02）
女	36	23（63.89）	13（36.11）
部位				0.048	0.827
左	65	32（49.23）	33（50.77）
右	32	15（46.88）	17（53.12）
病理类型				0.785	0.376
腺癌	82	43（52.44）	39（47.56）
非腺癌	15	6（40）	9（60）
肿瘤大小				0.011	0.917
＜5 cm	48	23（47.92）	25（52.08）
≥5 cm	49	24（48.98）	25（51.02）
浸润深度				4.194	0.041
T1+T2	7	6（85.71）	1（14.29）
T3+T4	90	41（45.56）	49（54.44）
N分期				6.395	0.011
N0	58	36（62.07）	22（37.93）
N1+2	39	14（35.90）	25（64.10）
M分期				0.003	0.957
M0	94	49（52.13）	45（47.87）
M1	3	1（33.33）	2（66.67）
TNM分期				7.284	0.007
Ⅰ+Ⅱ	56	36（64.29）	20（35.71）
Ⅲ+Ⅳ	41	15（36.59）	26（63.41）
脉管瘤栓				0.572	0.449
阴性	72	34（47.22）	38（52.78）
阳性	25	14（56.00）	11（44.00）
神经受侵				0.127	0.721
阴性	86	44（51.16）	42（48.84）
阳性	11	5（45.45）	6（54.55）
分化程度				0.335	0.563
中、高分化	83	41（49.40）	42（50.60）
低分化	12	7（58.33）	5（41.67）

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