Relationship between abnormal expression of Blimp-1 and SLAMF6 and immune function and pathogenesis of aplastic anemia patients

doi:10.3877/cma.j.issn.1674-0785.2022.04.005

Abstract

Abstract:

Objective

To explore the significance of expression of B-lymphocyte-induced mature protein (Blimp-1) and recombinant human SLAM family member 6 (SLAMF6) in patients with aplastic anemia (AA).

Methods

From January 2017 to June 2019, 89 AA patients (AA group) were selected at the First Affiliated Hospital of Xi'an Jiaotong University, including 51 non-severe AA patients and 38 severe AA patients. Fifty healthy people were selected as a control group. The expression of Blimp-1 mRNA was detected by RT-PCR, while the expression of SLAMF6 protein, CD3+ T cells, CD4+ T cells, CD8+ T cells, and regulatory T (Treg) cells was detected by flow cytometry.

Results

The relative expression of Blimp-1 mRNA and the expression of SLAMF6 protein, CD4+ T cells, and Treg cells in the AA group were (0.65±0.20), (55.10±11.82)%, (23.39±3.11)%, and (3.03±0.90) %, respectively, which were significantly lower than those of the control group (P＜0.05), while the expression of CD8+ T cells was (40.02±5.59)%, which was significantly higher than that of the control group (P＜0.05). The relative expression of Blimp-1 mRNA and the expression of SLAMF6 protein and Treg cells in severe AA patients were (0.45±0.12), (47.02±11.04)%, and (2.19±0.70)%, respectively, which were significantly lower than those in non-severe patients (P＜0.05), while the expression of CD8+ T cells was (47.54±7.21)%, which was significantly higher than that of non-severe patients (P＜0.05). The relative expression of Blimp-1 mRNA was positively correlated with the expression of Treg cells (r=0.589, P＜0.05), and the expression of SLAMF6 protein was negatively correlated with the expression of CD8+ T cells (r=-0.320, P＜0.05). After treatment, the relative expression of Blimp-1 mRNA and the expression of SLAMF6 protein, CD4+ T cells, and Treg cells in the AA group were (0.82±0.16), (62.01±10.12), (32.02±5.09)%, and (6.62±1.12)%, respectively, which were significantly higher than those before treatment (P＜0.05), while the expression of CD8+ T cells was (31.15±6.68)%, which was significantly lower than that before treatment (P＜0.05).

Conclusion

The expression of Blimp-1 and SLAMF6 is down-regulated in AA patients, which may be related to the severity of the disease. Blimp-1 expression is positively correlated with Treg cells, and SLAMF6 expression is negatively correlated with the expression of CD8+ T cells.

Key words: Aplastic anemia, B-lymphocyte-induced mature protein, Recombinant human SLAM family member 6, Immune function

Yangxin He, Ying Chen, Huaiyu Wang, Pengcheng He, Xiaoning Wang. Relationship between abnormal expression of Blimp-1 and SLAMF6 and immune function and pathogenesis of aplastic anemia patients[J]. Chinese Journal of Clinicians(Electronic Edition), 2022, 16(04): 312-318.

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Figures/Tables 9

组别	例数	性别［例（%）］		年龄（岁， $x ¯$ ±s）	身高（cm， $x ¯$ ±s）	体重（kg， $x ¯$ ±s）
组别	例数	男	女	年龄（岁， $x ¯$ ±s）	身高（cm， $x ¯$ ±s）	体重（kg， $x ¯$ ±s）
AA组	89	51（57.30）	38（42.70）	45.50±7.29	167.70±9.20	65.50±8.10
对照组	50	30（60.00）	20（40.00）	47.80±8.11	170.01±8.19	64.40±9.30
t/χ²值		0.096		-1.714	-1.477	0.728
P值		0.757		0.089	0.142	0.468

组别	例数	性别［例（%）］		年龄（岁， $x ¯$ ±s）	身高（cm， $x ¯$ ±s）	体重（kg， $x ¯$ ±s）
组别	例数	男	女	年龄（岁， $x ¯$ ±s）	身高（cm， $x ¯$ ±s）	体重（kg， $x ¯$ ±s）
AA组	89	51（57.30）	38（42.70）	45.50±7.29	167.70±9.20	65.50±8.10
对照组	50	30（60.00）	20（40.00）	47.80±8.11	170.01±8.19	64.40±9.30
t/χ²值		0.096		-1.714	-1.477	0.728
P值		0.757		0.089	0.142	0.468

组别	例数	Blimp-1 mRNA相对表达量	SLAMF6表达（%）	CD3+（%）	CD4+（%）	CD8+（%）	Treg（%）
AA组	89	0.65±0.20	55.10±11.82	65.50±6.06	23.39±3.11	40.02±5.59	3.03±0.90
对照组	50	1.03±0.19	78.82±9.92	67.01±5.89	41.10±5.60	25.59±5.10	8.10±1.88
t值		-10.943	-12.007	-1.424	-24.002	15.064	-21.475
P值		＜0.001	＜0.001	0.157	＜0.001	＜0.001	＜0.001

组别	例数	Blimp-1 mRNA相对表达量	SLAMF6表达（%）	CD3+（%）	CD4+（%）	CD8+（%）	Treg（%）
非重型	51	0.81±0.19	61.12±10.43	66.02±7.11	24.02±3.76	34.42±6.50	3.65±0.56
重型	38	0.45±0.12	47.02±11.04	64.80±6.82	22.49±4.05	47.54±7.21	2.19±0.70
t值		10.248	6.153	0.815	1.837	-8.989	10.929
P值		＜0.001	＜0.001	0.417	0.070	＜0.001	＜0.001

组别	例数	Blimp-1 mRNA相对表达量	SLAMF6表达（%）	CD3+（%）	CD4+（%）	CD8+（%）	Treg（%）
性别
男	51	0.67±0.18	53.20±8.28	64.49±7.82	23.50±3.35	41.10±8.10	3.00±0.65
女	38	0.61±0.13	56.60±7.04	66.10±8.03	22.93±3.50	39.93±7.94	3.09±0.60
t值		1.743	-2.040	-0.950	0.779	0.680	-0.667
P值		0.085	0.044	0.345	0.438	0.498	0.506
年龄
＜50岁	42	0.66±0.13	53.54±9.12	64.49±7.90	23.42±3.12	40.04±6.92	3.02±0.82
≥50岁	47	0.62±0.11	55.82±8.43	66.03±6.97	22.80±3.08	41.02±7.16	3.04±0.72
t值		1.572	-1.225	-0.977	0.942	-0.655	-0.123
P值		0.120	0.224	0.331	0.349	0.514	0.903

组别	Blimp-1 mRNA相对表达量	SLAMF6表达（%）	CD3+（%）	CD4+（%）	CD8+（%）	Treg（%）
治疗前	0.65±0.20	55.10±11.82	65.50±6.06	23.39±3.11	40.02±5.59	3.03±0.90
治疗后	0.82±0.16	62.01±10.12	66.04±5.92	32.02±5.09	31.15±6.68	6.62±1.12
t值	-6.262	-4.189	-0.601	-13.649	9.607	-23.572
P值	＜0.001	＜0.001	0.548	＜0.001	＜0.001	＜0.001

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