Significance of changes of peripheral blood miRNA-150 and CD19+CD24hiCD38hiBreg cells in children with immune thrombocytopenia

doi:10.3877/cma.j.issn.1674-0785.2022.04.007

Abstract

Abstract:

Objective

To explore the significance of changes of peripheral blood miRNA-150 and CD19⁺CD24^hiCD38^hiBreg cells in children with immune thrombocytopenia (ITP).

Methods

A total of 93 children with ITP treated at the Department of Hematology of Affiliated Maternity and Child Health Care Hospital of Nantong University from March 2017 to September 2020 were selected, including 56 cases with newly diagnosed ITP (course of disease＜3 months) and 37 cases with persistent ITP (course of disease 3-12 months). Another 50 healthy children were selected as a control group. The expression of miRNA-150 in peripheral blood was detected by real-time PCR, and the expression of CD19⁺CD24^hiCD38^hiBreg cells was detected by flow cytometry.

Results

The expression of miRNA-150 in peripheral blood of ITP children was higher than that of control group, while the expression rate of CD19⁺CD24^hiCD38^hiBreg cells was lower than of the control group (P＜0.05). The expression of miRNA-150 in the persistent ITP group was higher than that in the newly diagnosed ITP group, while the expression rate of CD19⁺CD24^hiCD38^hiBreg cells was lower than that in the newly diagnosed ITP group (P＜0.05). The expression of miRNA-150 was lower in the complete remission (CR) group than in the remission (R) group and no-remission (NR) group, and in the R group than in the NR group (P＜0.05). The expression rate of CD19⁺CD24^hiCD38^hiBreg cells was higher in the CR group than in the R group and NR group, and in the R group than in the NR group (P＜0.05). The sensitivity and specificity of peripheral blood miRNA-150 for diagnosing ITP were 78.18% and 60.53%, respectively, and those of CD19⁺CD24^hiCD38^hiBreg cells for diagnosing ITP were 83.63% and 71.05%, respectively. The expression miRNA-150 was negatively correlated with platelet count (r=-0.738), while CD19⁺CD24^hiCD38^hiBreg cells were positively correlated with platelet count (r=0.796).

Conclusion

The expression of miRNA-150 in peripheral blood is high in children with ITP, while the expression of CD19⁺CD24^hiCD38^hiBreg cells is low in children with ITP. The expression of miRNA-150 and CD19⁺CD24^hiCD38^hiBreg cells in peripheral blood is closely related to the curative effect.

Key words: miRNA-150, CD19⁺CD24^hiCD38^hiBreg cells, Immune thrombocytopenia in children

Yuanyuan Qian, Juan Zhang, Weigang Ji, Jinjun Zhou, Meiyun Zhou. Significance of changes of peripheral blood miRNA-150 and CD19⁺CD24^hiCD38^hiBreg cells in children with immune thrombocytopenia[J]. Chinese Journal of Clinicians(Electronic Edition), 2022, 16(04): 326-331.

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Figures/Tables 8

References 15

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指标	曲线下面积	截断值	敏感度（%）	特异度（%）
miRNA-150	0.959	0.89	78.18	60.53
CD19⁺CD24^hiCD38^hiBreg细胞	0.923	2.33	83.63	71.05

指标	曲线下面积	截断值	敏感度（%）	特异度（%）
miRNA-150	0.959	0.89	78.18	60.53
CD19⁺CD24^hiCD38^hiBreg细胞	0.923	2.33	83.63	71.05

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