Relationship between miR-34a/SIRT1 expression and cataract in rats

doi:10.3877/cma.j.issn.1674-0785.2017.13.005

Abstract

Abstract:

Objective

To detect the changes in the expression levels of miR-34a/SIRT1 and P53 in cataract lens in rats.

Methods

Thirty-six lenses from SD rats were divided into three groups: clear lenses of young and old rats and cataractous lenses. Expression of miR-34a/SIRT1 mRNA in the clear lenses of young and old rats and cataractous lenses was detected by real-time RT-PCR, and SIRT1 and P53 expression was detected by Western blot.

Results

Real-time PCR analysis showed that miR-34a expression was significantly up-regulated, accompanied by down-regulation of SIRT1 mRNA expression in rat cataractous lenses (t = 12.60, P = 0.0002; t = 3.299, P = 0.03, respectively). miR-34a expression was significantly lower (t = 9.609, P = 0.0007) and SIRT1 mRNA expression was significantly higher (t = 7.242, P = 0.0019) in clear lenses of old rats than in clear lenses of old lenses. Western blot analysis showed that SIRT1 protein expression was significantly down-regulated, accompanied by up-regulated expression of acetylated P53 protein in rat cataractous lenses (t = 8.924, P = 0.0009; t = 9.280, P = 0.0008, respectively). SIRT1 protein expression was significantly higher (t = 3.419, P = 0.0345) and acetylated P53 protein expression was significantly lower (t = 4.768, P = 0.0089) in clear lenses of old rats than in clear lenses of old lenses.

Conclusion

High expression of miR-34a is related to cataract in rats, possibly by decreasing SIRT1 protein expression and promoting the acetylation of P53.

Key words: Cataract, miR-34a/SIRT1, P53, Lens

Qingshan Ji, Xi Yu, Siqin Sun, Yuechun Wen, Genjie Ke. Relationship between miR-34a/SIRT1 expression and cataract in rats[J]. Chinese Journal of Clinicians(Electronic Edition), 2017, 11(13): 1982-1986.

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