AQP4 expression, apoptosis and brain edema in severe traumatic brain injury treated by traditional Chinese medicine and by refreshing method

doi:10.3877/cma.j.issn.1674-0785.2018.06.007

Abstract

Abstract:

Objective

To investigate the difference in AQP4 expression, apoptosis and brain edema between severe traumatic brain injury treated by refreshing method and by blood circulation activating method.

Methods

Adult Sprague-Dawley rats were randomly divided into a control group (12 rats), a model group (20 rats), a refreshing method group (20 rats), and a blood circulation activating method (20 rats). Severe traumatic brain injury was induced in rats by the improved Feeney′s methods. Brain samples were collected at 24 h, 48 h, 72 h and 7 d for measurement of water content. HE staining was performed to observe the changes in brain tissue. TUNEL method was used to detect apoptotic cells, and immunohistochemical method was used to detect the expression of AQP4 after injury. Besides, RT-PCR was utilized to validate the mRNA expression of AQP4 in different groups.

Results

The water content of brain tissue, the number of apoptotic cells around the focal brain injury and the expression of AQP4 at different times in the model group were significantly higher than those in the control group (P<0.05), while these indexes in the two treatment groups were significantly lower than those in the model group (P<0.05), with the refreshing method being superior to the blood circulation activating method. RT-PCR results suggested that AQP4 mRNA expression was similar to its protein expression pattern.

Conclusion

The changes in edema, apoptotic cells and expression of AQP4 after treatment for severe traumatic brain injury suggested that the refreshing method could improve the outome of severe traumatic brain injury, and it is better than the blood circulation activating method.

Key words: Severe traumatic brain injury, Aquaporins 4, Edema, Refreshing Method

Min Wei, Hao Meng, Lun Dong, Xingdong Wang, Zhengcun Yan, Hengzhu Zhang. AQP4 expression, apoptosis and brain edema in severe traumatic brain injury treated by traditional Chinese medicine and by refreshing method[J]. Chinese Journal of Clinicians(Electronic Edition), 2018, 12(06): 347-353.

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Figures/Tables 6

References 13

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组别	鼠数	伤后24 h	伤后48 h	伤后72 h	伤后7 d
假手术组	12	71.51±2.88	71.53±2.97	71.63±2.76	71.57±2.86
模型组	20	80.02±0.54^b	83.42±0.48^b	83.50±0.56^b	80.50±1.49^b
传统活血组	20	79.40±0.72	80.81±0.80^a	80.71±0.81^a	77.97±0.81^a
醒脑静治疗组	20	77.85±0.79^a	80.68±0.90^a	77.42±0.80^ac	75.47±1.44^ac

组别	鼠数	伤后24 h	伤后48 h	伤后72 h	伤后7 d
假手术组	12	71.51±2.88	71.53±2.97	71.63±2.76	71.57±2.86
模型组	20	80.02±0.54^b	83.42±0.48^b	83.50±0.56^b	80.50±1.49^b
传统活血组	20	79.40±0.72	80.81±0.80^a	80.71±0.81^a	77.97±0.81^a
醒脑静治疗组	20	77.85±0.79^a	80.68±0.90^a	77.42±0.80^ac	75.47±1.44^ac

组别	鼠数	伤后24 h	伤后48 h	伤后72 h	伤后7 d
假手术组	12	0.43±0.01	0.46±0.01	0.43±0.01	0.44±0.01
模型组	20	0.73±0.01	1.43±0.04	1.21±0.12	0.98±0.08
传统活血组	20	0.75±0.08	1.23±0.10^a	1.05±0.08^a	0.93±0.10
醒脑静治疗组	20	0.54±0.05^ac	0.74±0.04^ac	0.72±0.03^ac	0.61±0.02^ac

组别	鼠数	伤后24 h	伤后48 h	伤后72 h	伤后7 d
假手术组	12	0.43±0.01	0.46±0.01	0.43±0.01	0.44±0.01
模型组	20	0.73±0.01	1.43±0.04	1.21±0.12	0.98±0.08
传统活血组	20	0.75±0.08	1.23±0.10^a	1.05±0.08^a	0.93±0.10
醒脑静治疗组	20	0.54±0.05^ac	0.74±0.04^ac	0.72±0.03^ac	0.61±0.02^ac

组别	鼠数	伤后24 h	伤后48 h	伤后72 h	伤后7 d
假手术组	12	4.80±3.93	3.60±2.69	4.20±2.51	3.20±2.78
模型组	20	176.24±10.16	281.80±10.89	224.48±16.56	217.56±15.66
传统活血组	20	155.28±8.53^a	228.44±17.07^a	216.32±12.64^a	191.60±8.81^a
醒脑静治疗组	20	143.52±10.16^ac	197.08±10.55^ac	182.08±7.94^ac	154.52±6.64^ac

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