MiRNA-224-5p regulates apoptosis of papillary thyroid carcinoma cells via the transforming growth factor beta/Smad4 signaling pathway

doi:10.3877/cma.j.issn.1674-0785.2018.11.004

Abstract

Abstract:

Objective

To explore the role of miRNA-224-5p in the apoptosis of papillary thyroid carcinoma cells and the underlying mechanism.

Methods

The expression of miRNA-224-5p in 60 cases of papillary thyroid carcinoma and adjacent tissue was detected by RT-PCR. Human papillary thyroid carcinoma GLAG-66 cells were cultured, miRNA-224-5p and miRNA-224-5p inhibitor were transfected into cells, and the expression of miRNA-224-5p was detected by RT-PCR. Cell apoptosis was detected by flow cytometry 48 h later. The expression of Bcl-2, Bax, Cleaved-Caspase3, TGF-β1, and Smad4 proteins was detected by Western blot.

Results

The expression of miRNA-224-5p in papillary thyroid carcinoma tissue was significantly higher than that in paracancerous tissue [(4.175±0.523) vs (1.236±0.236), t=9.675, P=0.001], and the expression of miRNA-224-5p was significantly lower in the miRNA-224-5p inhibitor group than in the miRNA-224-5p group [(0.381±0.078) vs (1.000±0.023), t=19.785, P<0.001)]. Compared with the miRNA-224-5p group, the apoptosis rate significantly increased in the miRNA-224-5p inhibitor group [(18.66%±0.98%) vs (3.78%±0.36%), t=17.561, P<0.001]. Compared with the miRNA-224-5p group, the expression of Bcl-2, TGF-β1 and Smad4 in the miRNA-224-5p inhibitor group was significantly decreased [(0.197±0.008) vs (0.278±0.013), t=8.259, P=0.001; (0.117±0.013) vs (0.227±0.012), t=13.269, P<0.001; (0.268±0.012) vs (0.439±0.016), t=9.897, P=0.001], and the expression of Bax and Cleaved-Caspase3 protein was significantly increased [(0.286±0.011) vs (0.159±0.009), t=12.569, P<0.001; (0.128±0.009) vs (0.083±0.010), t=7.693, P=0.002].

Conclusion

MiRNA-224-5p is highly expressed in papillary thyroid carcinoma, and down-regulation of miRNA-224-5p promotes cell apoptosis via mechanisms possibly related to regulation of the TGF-β/Smad4 signaling pathway.

Key words: MiRNA-224-5p, Papillary thyroid carcinoma, TGF-β/Smad4 signaling pathway, Apoptosis

Nan Ma, Rong Xu, Pengcheng Su. MiRNA-224-5p regulates apoptosis of papillary thyroid carcinoma cells via the transforming growth factor beta/Smad4 signaling pathway[J]. Chinese Journal of Clinicians(Electronic Edition), 2018, 12(11): 609-613.

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URL: https://zhlcyszz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0785.2018.11.004

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References 25

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