In the human genome, at least 70% of DNA will be transcribed into RNA, but less than 2% of DNA can encode proteins, and most of the DNA will eventually be transcribed into non-coding RNAs (ncRNAs). In the past few decades, ncRNAs were thought to be useless and play no role in the process of life. But in recent years, with the deepening of the research and the development of sequencing technology, more and more evidence shows that ncRNAs play an important regulatory role at the epigenetic, transcriptional, and post-transcriptional levels. Pulpitis is one of the most common symptoms in clinical stomatology. Currently, tooth extraction or root canal therapy is the common treatment for the pulpitis. However, these methods are very destructive to the teeth, so new methods should be explored to protect the dental pulp as much as possible. The advantages of ncRNAs in the diagnosis and evaluation of pulpitis are increasingly recognized. A large number of studies have shown that ncRNAs play an important role not only in the occurrence and development of pulpitis, but also in the control of inflammation and the reduction of the response of stimulating factors to cell damage. The purpose of this review is to elucidate the relationship between ncRNAs and pulpitis, and to explore their application in the treatment of pulpitis.