Abstract:
Objective To investigate the factors that affect the collection of non-mobilized peripheral blood stem cells (PBSCs).
Methods Autologous PBSCs without mobilization with granulocyte colony stimulating factor (G-CSF) were collected from 56 patients with solid tumor and 53 healthy donors at the Affiliated Tongji Hospital of Tongji University from January 2016 to June 2018 using an MCS+ blood cell separator. White blood cells (WBC), hematocrit (Hct), platelets (PLT), hemoglobin (Hb), mononuclear cells (MNC), and collection index before and after collection were compared between the two groups. The correlation between collection effects and some influencing factors was analyzed.
Results A certain number of MNC and CD34+ cells were successfully collected in both groups. Hct, PLT, and Hb in the healthy group were significantly higher than those in the patient group [(44.81±3.99)% (38.38±4.49)%; (230.91±44.63)×109/kg vs (202.88±60.39)×109/kg; (146.09±16.62)g/L vs (129.07±15.54) g/L; t=7.84, 2.74, 5.51, P<0.05)]. The total number of MNC and WBC in the control group was significantly higher than that in the patient group [(2.81±1.35)×108/kg vs (1.99±1.05)×108/kg; (92.23±29.46)×109/L vs (54.60±23.61)×109/L; t=3.54, 7.35, P<0.05], but the percentages of CD34+ cells and MNC were significantly lower than those in the patient group [(1.17±0.66)% vs (1.65±1.01)%; (53.68±20.19)% vs (65.11±16.45)%; t=2.91, 3.24, P<0.05]. The total number of CD34+ cells and MNC in the healthy group was negatively correlated with the pre-harvest Hct index (r=-0.30915, -0.19624; P=0.00004, 0.01119), and the number of CD34+ cells was negatively correlated with the total volume of blood collected and the body mass of the collected patients (r=-0.04114, -0.15436, P=0.02612, 0.03071). The total number of CD34+ cells and MNC in the patient group was positively correlated with the pre-harvest PLT count and the body mass index (r=0.14135, 0.19609; r=0.04678, 0.14765, P<0.05). The number of CD34+ cells was positively correlated with WBC count and body mass before harvest (r=0.02028, 0.18605, P<0.05).
Conclusion There is a correlation between the collection of non-mobilized PBSCs and WBC, PLT, Hct, body mass index, product volume, and the amount of blood circulation. The total number of MNC and WBC in healthy controls are significantly higher than those in the patient group.
Key words:
Solid tumor,
Healthy donor,
Non-mobilization,
Peripheral blood stem cells,
Collection
Liming Ji, Zimin Lu, Qi Fang, Chengzhong Liu, Xuechen Li. Factors influencing collection of non-mobilized peripheral blood stem cells from solid tumor patients and healthy donors[J]. Chinese Journal of Clinicians(Electronic Edition), 2019, 13(06): 419-423.