Clinical significance of expression of plasmacytoid dendritic cells and interferon-γ in labial gland tissue of patients with Sjogren′s syndrome

doi:10.3877/cma.j.issn.1674-0785.2019.06.008

Abstract

Abstract:

Objective

To determine the expression of plasmacytoid dendritic cells and interferon-gama (IFN-γ) in the labial gland tissue of patients with primary Sjogren′s syndrome (pSS) to investigate the role of pDC in the pathogenesis of pSS and their significance.

Methods

Gland tissue samples were collected from 39 patients with pSS and 19 patients with dry mouth who did not meet the diagnostic criteria for pSS. pDC and IFN-γ were determined by fluorescent antibody technique to assess the association between pDC and lymphocyte focal index as well as the association between pDC and clinical indexes of pSS patients. The difference between the two groups of data was measured by the rank-sum test, and Spearman bivariate correlation analysis was performed to examine the correlation between variables.

Results

A large number of pDC cells were observed in the lip gland tissue of patients with pSS, and the count of pDC in the study group was significantly higher than that of the control group [103 (76, 203) vs 46 (39, 50), Z=-5.602, P<0.001)]. There was unusually high expression of IFN-γ in the study group, and the count of IFN-γ positive cells was also significantly higher in the study group than in the control group [174 (137, 214) vs 116 (112, 120), Z=-5.783, P<0.001)]. There was a positive correlation between pDC counts and lymphocyte focal index (r=0.810, P<0.001) as well as between pDC counts and the expression of IFN-γ (r=0.894, P<0.001), although there was no significant correlation between pDC and clinical indexes (P>0.05).

Conclusion

Both massive infiltration of pDC and overexpression of interferon are closely correlated with lymphocytic infiltration in labial gland tissue of patients with pSS. pDC are involved in the immune injury of labial gland tissue. Detection of pDC may be conducive to early pathological diagnosis of pSS.

Key words: Primary Sjogren′s syndrome, Plasmacytoid dendritic cells, Interferon gama, Immunofluorescent labeling, Laser confocal

Shancui Zheng, Kaiwen Wang, Lei Liu, Zhiyong Deng, Fang Chen, Xueming Sun, Junjun Cao, Jia Tang. Clinical significance of expression of plasmacytoid dendritic cells and interferon-γ in labial gland tissue of patients with Sjogren′s syndrome[J]. Chinese Journal of Clinicians(Electronic Edition), 2019, 13(06): 444-448.

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References 15

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组别	例数	pDC计数	IFN-γ
研究组	39	103（76，203）	174（137，214）
对照组	19	46（39，50）	116（112，120）
Z值	?	-5.602	-5.783
P值	?	<0.001	<0.001
抗SSA/SSB抗体阳性组	26	101（77，181）	151（141.5，203.5）
抗SSA/SSB抗体阴性组	13	169（72.5，216）	196（131.5，224）
Z值	?	-1.028	-0.820
P值	?	0.304	0.412

组别	例数	pDC计数	IFN-γ
研究组	39	103（76，203）	174（137，214）
对照组	19	46（39，50）	116（112，120）
Z值	?	-5.602	-5.783
P值	?	<0.001	<0.001
抗SSA/SSB抗体阳性组	26	101（77，181）	151（141.5，203.5）
抗SSA/SSB抗体阴性组	13	169（72.5，216）	196（131.5，224）
Z值	?	-1.028	-0.820
P值	?	0.304	0.412

相关指标	淋巴细胞灶数	IFN-γ	ESSDAI	血沉	C反应蛋白	IgA	IgG	ANA
r值	0.810	0.894	0.235	0.182	-0.021	0.079	0.063	0.114
P值	<0.001	<0.001	0.150	0.275	0.913	0.780	0.804	0.496

相关指标	淋巴细胞灶数	IFN-γ	ESSDAI	血沉	C反应蛋白	IgA	IgG	ANA
r值	0.810	0.894	0.235	0.182	-0.021	0.079	0.063	0.114
P值	<0.001	<0.001	0.150	0.275	0.913	0.780	0.804	0.496

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