Home    中文  
 
  • Search
  • lucene Search
  • Citation
  • Fig/Tab
  • Adv Search
Just Accepted  |  Current Issue  |  Archive  |  Featured Articles  |  Most Read  |  Most Download  |  Most Cited

Chinese Journal of Clinicians(Electronic Edition) ›› 2021, Vol. 15 ›› Issue (04): 272-279. doi: 10.3877/cma.j.issn.1674-0785.2021.04.007

• Basic Science Research • Previous Articles     Next Articles

Effects of Active Extracts of Miaoyao Fanggan Sachet on bronchial epithelial cells stimulated with polymyocytic acid

Xiaofen Zhang1, Quan Zhang2,()   

  1. 1. Clinical Medical College, Guizhou Medical University, Guiyang 550001, China
    2. Department of Infection, Affiliated Hospital of Guizhou Medical University, Guiyang 550001, China
  • Received:2021-03-24 Online:2021-04-15 Published:2021-08-06
  • Contact: Quan Zhang

Abstract:

Objective

To investigate the effects of diammonium glycyrrhizinate (DG) and magnesium isoglycyrrhizinate (MI) on bronchial epithelial cells (NHBE) stimultated with polymyocytic acid (PIC), to determine the therapeutic effects of DG and MI and to explore the possible mechanisms involved.

Methods

NHBE cells were stimulated with 1 μg/ml PIC to construct the cell model. Cells treated with Dexamethasone (DXMS) at 10 μg/ml were used as a positive control. DG and MI at low, medium, and high concentrations (0.5, 1.0, 2.0 mg/ml) were used for cell intervention. Cell activity was detected with CCK8 kit. IFN-α and IL-6 levels were detected by ELISA. IFN-α and IL-6 mRNA levels were detected by qPCR. p-TAK1/TAK1 and p-IkB/IkB expression levels were detected by Western blot.

Results

CCK8 assay showed that the viability of NHBE cells decreased with the increase of drug concentration, and the decrease of cell viability was more obvious in the positive control group. Compared with blank control cells, the expression of IFN-α and IL-6 significantly increased in the model group (cells treated with PIC; P<0.05). Compared with the model group, the expression levels of IFN-α and IL-6 significantly decreased after treatment with different doses of DG and MI (P<0.05). The mRNA expression levels of IFN-α and IL-6 in the model group were significantly higher compared with the blank control group (P<0.05), but these in the intervention groups were significantly decreased (P<0.05). After modeling, the ratios of p-TAK1/TAK1 and p-IkB/IkB tended to increase significantly (P<0.05), while they showed no signficant changes in NHBE cells treated with low, medium, and high concentrations of DG and MI. In the positive control group, these ratios decreased compared with those in the model group (P<0.05).

Conclusion

Ammonium glycyrrhizinate is one of the main active components of Miaoyao Fanggan Sachet. DG and MI have certain curative effects on PIC induced NHBE proinflammatory response possibly by downregulating IFN-α and IL-6 expression and reducing the inflammatory response induced by PIC. Whether the mechamism of PIC induced pro-inflammatory response of NHBE cells is related to the phosphorylation levels of TAK1 and IKB proteins needs to be further studied.

Key words: Miaoyao Fanggan Sachet, Polymyocytic acid, Diammonium glycyrrhizinate, Magnesium isoglycyrrhizinate

京ICP 备07035254号-20
Copyright © Chinese Journal of Clinicians(Electronic Edition), All Rights Reserved.
Tel: 010-57830845 E-mail: zhlcyszz@cma.org.cn
Powered by Beijing Magtech Co. Ltd