The construction of national regional medical centers, a key initiative aimed at optimizing the allocation of medical resources and enhancing regional medical service capabilities, has created new opportunities for discipline development in large public hospitals. Taking Zhejiang Provincial People's Hospital as an example, this paper systematically examines its strategic planning, implementation approaches, and practical achievements in discipline construction during the period of national regional medical center construction. The research shows that by adopting measures such as formulating a "two hearts and four wings" development strategy, prioritizing breakthroughs in cutting-edge technologies, establishing high-level scientific research and innovation platforms, refining talent recruitment and training mechanisms, and strengthening regional radiation and leadership, the hospital has significantly enhanced its discipline strength. This has resulted in a replicable and scalable model for high-quality discipline development, offering valuable insights for the discipline construction of other regional medical centers.