重复流产对F1代雄鼠生长发育及生育能力的影响

doi:10.3877/cma.j.issn.1674-0785.2021.07.010

中华临床医师杂志(电子版) ›› 2021, Vol. 15 ›› Issue (07) : 532 -536. doi: 10.3877/cma.j.issn.1674-0785.2021.07.010

基础研究

重复流产对F1代雄鼠生长发育及生育能力的影响

吕芳¹, 王丽丽², 王欣³, 王宁², 张树成²^,^†(

)

^1. 215004　江苏苏州，苏州大学附属第二医院生殖医学中心；100081　北京，国家卫健委科学技术研究所生殖生理室
^2. 100081　北京，国家卫健委科学技术研究所生殖生理室
^3. 225003　江苏扬州，扬州大学医学院

收稿日期:2021-05-26 出版日期:2021-07-15
通信作者: 张树成
基金资助:
国家自然科学青年基金(81601343); 国家科技基础性工作专项(2013FY110500); 苏州大学附属第二医院科教兴院人才托举项目(XKTJ-RC202002)

Impact of repeated abortions on growth and reproductive capacity of F1 generation male mice

Fang Lv¹, Lili Wang², Xin Wang³, Ning Wang², Shucheng Zhang²^,^†()

^1. Reproductive Medicine Center, the Second Affiliated Hospital of Soochow University, Soochow 215004, China; Department of Reproductive Physiology, National Research Institute for Family Planning, Beijing 100081, China
^2. Department of Reproductive Physiology, National Research Institute for Family Planning, Beijing 100081, China
^3. Yangzhou University Medical College, Yangzhou 225003, China

Received:2021-05-26 Published:2021-07-15
Corresponding author: Shucheng Zhang

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引用本文：

吕芳, 王丽丽, 王欣, 王宁, 张树成. 重复流产对F1代雄鼠生长发育及生育能力的影响[J]. 中华临床医师杂志(电子版), 2021, 15(07): 532-536.

Fang Lv, Lili Wang, Xin Wang, Ning Wang, Shucheng Zhang. Impact of repeated abortions on growth and reproductive capacity of F1 generation male mice[J]. Chinese Journal of Clinicians(Electronic Edition), 2021, 15(07): 532-536.

目的

探讨重复流产对F1代雄鼠生长发育情况和交配繁殖能力的影响。

方法

将经历2次药物流产之后的BALB/c小鼠再次妊娠后分娩的F1代雄鼠作为实验组，未经历流产小鼠分娩的F1代作为对照组。比较出生3周后，2组F1代小鼠存活率和体质量增长情况。比较2组F1代雄鼠睾丸发育情况、附睾精子密度、精子活力以及重复流产后F1代雄鼠交配生殖能力的变化。

结果

与对照组比较，实验组F1代小鼠存活率、生长发育状况无显著差异（P＞0.05），但实验组F1代雄鼠睾丸重量（P＜0.01）、精子密度（P＜0.05）、精子活力（P＜0.05）显著低于对照组，并且交配合笼能力显著低于对照组（P＜0.05），提示实验组F1代雄鼠生殖系统发育和生育能力降低。

结论

母本重复流产经历将会影响F1代雄鼠的生殖系统发育和生育能力。

关键词: 重复流产, 精子活率, F1代, 生育力

Objective

To investigate the impact of repeated abortions on the growth and reproductive capacity of F1 generation male mice.

Methods

F1 generation male mice born by BALB/c mice after two medical abortions induced by intraperitoneal injection of RU486 were used as an experimental group, and F1 generation male mice born by BALB/c mice that had no history of abortions were used as a control group. The survival rate and body weight of F1 generation neonates during the first three weeks after birth were measured. The testicular weight, testicular morphology, epididymis sperm concentration, sperm motility, and reproductive ability of F1 generation male mice were compared between the two groups.

Results

There was no significant difference in neonate survival rate or body weight gain between the two groups (P＞0.05), but the reproductive capacity paramters, including the testicular weight (P＜0.01), sperm concentration (P＜0.05), sperm activity (P＜0.05), and the mating ability (P＜0.05), were significantly lower in the experimental group than in the control group, indicating that the development of the reproductive system and fertility of F1 generation male mice decreased in the experimental group.

Conclusion

The reproductive system development and fertility of F1 male mice can be impaired by repeated abortions of mothers.

Key words: Repeated abortion, Sperm motility, F1 generation, Fertility

图1 F1代小鼠出生至21 d离乳体质量变化曲线

图2 2组F1代雄鼠精子形态观察及睾丸重量、精子密度及精子活力比较。图a为C_G1P1_F1雄鼠精子形态；图b为T_G3P1_F1雄鼠精子形态（箭头所示为小鼠精子）；图c为2组F1代雄鼠精子密度比较；图d为2组F1代雄鼠精子活力比较；图e为2组F1代雄鼠睾丸重量比较

图3 2组F1代雄鼠睾丸大体形态及HE染色情况。图a为C_G1P1_F1小鼠睾丸组织大体形态；图b为T_G3P1_F1中无精症小鼠睾丸组织大体形态；图c为C_G1P1_F1小鼠睾丸组织HE染色（×400）；图d为T_G3P1_F1小鼠睾丸组织HE染色（×400）；图e为T_G3P1_F1中无精症小鼠睾丸HE染色（×400）

表1 T_G3P1_F1与C_G1P1_F1生殖交配能力比较

组别	雄鼠数（只）	检栓天数（d）	合笼雌鼠（只）	见栓雌鼠（只）	妊娠雌鼠（只）	见栓后妊娠率（%）	着床点数目（ $x ¯$ ±s）	吸收胎率（%， $x ¯$ ±s）	活胎数（只， $x ¯$ ±s）	出生体质量（g， $x ¯$ ±s）
C_G1P1_F1	23	16	75	34	21	61.8	7.0±0.6	15.3±4.8	5.9±0.2	1.41±0.13
T_G3P1_F1	20	16	75	19	13	68.4	6.8±0.8	28.6±9.9	5.0±0.3	1.40±0.22

表1 T_G3P1_F1与C_G1P1_F1生殖交配能力比较

组别	雄鼠数（只）	检栓天数（d）	合笼雌鼠（只）	见栓雌鼠（只）	妊娠雌鼠（只）	见栓后妊娠率（%）	着床点数目（ $x ¯$ ±s）	吸收胎率（%， $x ¯$ ±s）	活胎数（只， $x ¯$ ±s）	出生体质量（g， $x ¯$ ±s）
C_G1P1_F1	23	16	75	34	21	61.8	7.0±0.6	15.3±4.8	5.9±0.2	1.41±0.13
T_G3P1_F1	20	16	75	19	13	68.4	6.8±0.8	28.6±9.9	5.0±0.3	1.40±0.22

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