Abstract:
Objective To test the resistance of Mycobacterium tuberculosis to isoniazid (INH), rifampicin (RFP), ethambutol (EMB,) and streptomycin (SM) by using microplate allochroic silica gel assay to assess its clinical application value.
Methods One hundred and fifty clinical isolates of Mycobacterium tuberculosis from Nantong Sixth People′s Hospital were applied to test their resistance to the four kinds of first-line antituberculous drugs by microplate allochroic silica gel assay, and the results were compared with those obtained by the Bactec MGIT method.
Results The best concentration of MTB in microwell is 10-3 mg/ml. The time of detection for microplate allochroic silica gel assay was 7-10 days. The best thresholds for detecting drug susceptibility of INH, RFP, EMB, and SM were 0.25 μg/ml, 1 μg/ml, 4 μg/ml, and 2 μg/ml, respectively. According to the results of Bactec MGIT method, the accuracies of the microplate allochroic silica gel assay were 96.7%, 98.0%, 92.0%, and 96.0%, respectively.
Conclusion The microplate allochroic silica gel assay for detecting drug resistance of Mycobacterium tubercu1osis is accurate, rapid, inexpensive, and simple, and can meet the need of rapid clinical diagnosis.
Key words:
Mycobacterium tuberculosis,
Isoniazid,
Rifampicin,
Ethambutol,
Streptomycin,
Microplate allochroic silica gel assay
Fei Huang, Junlin Chen, Delin Gu, Mei Qu, Zhongyi Hu, Yuansheng Ding. Testing of drug resistance of Mycobacterium tuberculosis by microplate allochroic silica gel assay[J]. Chinese Journal of Clinicians(Electronic Edition), 2017, 11(20): 2301-2306.