Diagnosis of lymphatic tuberculosis and drug resistance analysis by gene chip technology

doi:10.3877/cma.j.issn.1674-0785.2020.11.008

Abstract

Abstract:

Objective

To assess the clinical value of gene chip technology in the diagnosis of lymphatic tuberculosis and analysis of drug resistance.

Methods

Eighty patients with suspected lymphatic tuberculosis treated at Ganzhou Fifth People's Hospital from August 2016 to October 2019 were included. Bacterial identification was performed in all patients using gene chip technology and culture method. Strains that have been identified as belonging to the Mycobacterium tuberculosis (MTB) composite group were used to conduct rifampin and isoniazid resistance testing. Clinical symptoms were recorded. Using the results of traditional Roche solid culture method as the reference standard, the diagnostic coincidence rate of gene chip technology for the identification of nontuberculous Mycobacteria (NTM). Then, the coincidence rate, sensitivity, and specificity of gene chip technology for the detection of rifampicin and isoniazid resistance were calculated.

Results

The main clinical manifestations of 80 patients with suspected lymphoid tuberculosis included mass, chills, emaciation, expectoration, and suffocation, accounting for 78.75% (63/80), 32.50% (26/80), 31.25% (25/80), 33.75% (27/80), and 30.00% (24/80) of all cases, respectively. The diagnostic coincidence rate of gene chip technology for NTM identification in patients with lymphoid tuberculosis was 100%. The sensitivity, specificity, and coincidence rate of gene chip technology in detecting rifampicin were 98.57% (69/70), 100.00% (17/17), and 98.11% (52/53), respectively, which had no significant difference from those of the traditional Roche solid culture method (P＞0.05). The sensitivity, specificity, and coincidence rate of gene chip technology in detecting isoniazid resistance were 98.57% (69/70), 100.00% (23/23), and 97.87% (46/47), respectively, which had no significant difference from those of the traditional Roche solid culture method (P＞0.05).

Conclusion

Gene chip technology has high application value in the diagnosis of lymphoid tuberculosis, and its efficiency for diagnosis of lymphoid tuberculosis and detection of MTB drug resistance is basically comparable to that of the traditional culture method.

Key words: Lymphatic tuberculosis, Mycobacterium tuberculosis, Gene chip technology, Drug resistance detection

Zhong Zeng, Jing Zhou, Jun Tao, Haibin Lai. Diagnosis of lymphatic tuberculosis and drug resistance analysis by gene chip technology[J]. Chinese Journal of Clinicians(Electronic Edition), 2020, 14(11): 890-894.

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References 17

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临床表现	例数	百分比（%）	临床表现	例数	百分比（%）
发热	6	7.50	咳嗽	13	16.25
低热	7	8.75	咯痰	27	33.75
高热	6	7.50	胸痛	16	20.00
畏寒	26	32.50	憋气	24	30.00
盗汗	8	10.00	头痛	6	7.50
消瘦	25	31.25	腹胀	10	12.50
咯血	13	16.25	肿块	63	78.75
关节肿痛	11	13.75	腰痛	9	11.25

临床表现	例数	百分比（%）	临床表现	例数	百分比（%）
发热	6	7.50	咳嗽	13	16.25
低热	7	8.75	咯痰	27	33.75
高热	6	7.50	胸痛	16	20.00
畏寒	26	32.50	憋气	24	30.00
盗汗	8	10.00	头痛	6	7.50
消瘦	25	31.25	腹胀	10	12.50
咯血	13	16.25	肿块	63	78.75
关节肿痛	11	13.75	腰痛	9	11.25

方法	符合率	敏感度	特异度
基因芯片技术	98.57（69/70）	100.00（17/17）	98.11（52/53）
培养法	100.00（70/70）	100.00（18/18）	100.00（52/52）
χ²值	1.440	0.001	1.908
P值	0.230	1.000	0.167

方法	符合率	敏感度	特异度
基因芯片技术	98.57（69/70）	100.00（17/17）	98.11（52/53）
培养法	100.00（70/70）	100.00（18/18）	100.00（52/52）
χ²值	1.440	0.001	1.908
P值	0.230	1.000	0.167

方法	符合率	敏感度	特异度
基因芯片技术	98.57（69/70）	100.00（23/23）	97.87（46/47）
培养法	100.00（70/70）	100.00（24/24）	100.00（46/46）
χ²值	1.440	0.001	2.153
P值	0.230	1.000	0.142

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