Expression of glycine decarboxylase in p53-mutated B cell lymphoma in mice

doi:10.3877/cma.j.issn.1674-0785.2018.12.007

Abstract

Abstract:

Objective

To understand the relationship between p53-mutated B cell lymphoma and glycine decarboxylase (GLDC) expression.

Methods

Based on immunohistochemical staining results, BALB/c nude mice were divided into a p53 protein-positive group and 53 protein-negative group. GLDC mRNA expression was determined by real-time polymerase chain reaction, and GLDC protein expression was determined by Western blot. We designed a GLDC-specific siRNA to transfect human B cell lymphoma cell line (Raji). The t-test was used for statistical analyses.

Results

Both GLDC mRNA and protein expression in B cell lymphoma tissue were higher in the p53 protein-positive group than in the p53 protein-negative group, and there was a significant difference in the expression of GLDC mRNA between the two groups (2.38±0.26 vs 11.32±1.65, t=6.74, P<0.01).The proliferative ability of GLDC siRNA-transfected cells (0.22±0.01) decreased significantly compared with the negative-control siRNA group (0.61±0.06) and the blank control group (0.51±0.04) (t=3.74, P<0.05).

Conclusion

These findings support a direct relationship between p53 mutation and GLDC expression in B cell lymphoma. GLDC gene can promote cell proliferation in p53-mutated B cell lymphoma and thus may provide a new target for the treatment of B-cell lymphoma.

Key words: B-cell lymphoma, p53 gene, Glycine decarboxylase, Small interfering RNA

Tao Sun, Xiangdong Li, Peng Liu, Feng Liu, Shuomin Li. Expression of glycine decarboxylase in p53-mutated B cell lymphoma in mice[J]. Chinese Journal of Clinicians(Electronic Edition), 2018, 12(12): 684-687.

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Figures/Tables 4

References 14

1	SPIKE BT, Wahl GM. p53, stem cells, and reprogramming: tumor suppression beyond guarding the genome [J]. Genes Cancer, 2011, 2(4): 404-419.
2	Swerdlow SH, Campo E, Harris NL, et al. WHO Classificationof Tumours of Haematopoietic and LymphoidTissues[M]. Lyon, France: IARC Press, 2008: 326-332
3	Adams CM, Eischen CM. Inactivation of p53 is insufficient to allow B cells and B-cell lymphomas to survive without Dicer [J]. Cancer Res, 2014, 74(14): 3923-3934.
4	Huang X, Meng B, Iqbal J, et al. Activation of the STAT3 signaling pathway is associated with poor survival in diffuse large B-cell lymphoma treated with R-CHOP [J]. J Clin Oncol, 2013, 31(36): 4520-4528.
5	Jiang DF, Aguiar RC. MicroRNA-155 controls RB phosphorylation in normal and malignant B lymphocytes via the noncanonical TGF-β1/SMAD5 signaling module [J]. Blood, 2014, 123(1): 86-93.
6	De Miranda NF, Georgiou K, Chen LY, et al. Exome sequencing reveals novel mutation targets in diffuse large B-cell lymphomas derived from Chinese patients [J]. Blood, 2014, 124(16): 2544-2553.
7	Zhang WC, Shyh-Chang N, Yang HE, et al. Glycine decarboxylase activity drives non-small cell lung cancer tumor-initiating cells and tumorigenesis [J]. Cell, 2012, 148(1/2): 259-272.
8	Dominy JE, Vazquez F, Puigserver P. Glycine decarboxylase cleaves a ″malignant″ metabolic path to promote tumor initiation [J]. Cancer Cell, 2012, 21(2): 143-145.
9	Pai YJ, Leung KY, Savery D, et al. Glycine decarboxylase deficiency causes neural tube defects and features of non-ketotic hyperglycinemia in mice [J]. Nat Commun, 2015, 3(6): 6388-6399.
10	Timm S, Florian A, Arrivault S, et al. Glycine decarboxylase controls photosynthesis and plant growth [J]. FEBS Lett, 2012, 586(20): 3692-3697.
11	Vogelstein B, Kinzler KW. Cancer genes and the pathways they control [J]. Nat Med, 2004, 10(8): 789-799.
12	Hanahan D, Weinberg RA. Hallmarks of cancer: the next Generation [J]. Cell, 2011, 144(5): 646-674.
13	Chang H, Jiang AM, Qi CX. Aberrant nuclear p53 expression predicts hemizygous 17p (Tp53) deletion in chronic lymphocytic leukemia [J]. Am J Clin Pathol, 2010, 133(1): 70-74.
14	Kume A, Koyata H, Sakakibara T, et al. The glycine cleavage system. Molecular cloning of the chicken and human glycine decarboxylase cDNAs and some characteristicsinvolved in the deduced protein structures [J]. J Biol Chem, 1991, 266(5): 3323-3329.

组别	数量	GLDC mRNA	GLDC蛋白
p53蛋白阴性组	29	2.38±0.26	4.36±0.42
p53蛋白阳性组	19	11.32±1.65	23.42±2.26
t值	?	5.63	6.74
P值	?	<0.01	<0.01

组别	数量	GLDC mRNA	GLDC蛋白
p53蛋白阴性组	29	2.38±0.26	4.36±0.42
p53蛋白阳性组	19	11.32±1.65	23.42±2.26
t值	?	5.63	6.74
P值	?	<0.01	<0.01

组别	24 h	48 h	72 h	96 h
空白对照组	0.45±0.03	0.45±0.03	0.45±0.04	0.51±0.04
阴性对照siRNA组	0.49±0.03	0.52±0.04	0.62±0.05	0.61±0.06^a
GLDC siRNA转染组	0.50±0.03	0.48±0.03	0.54±0.04	0.22±0.01^b
F值	1.23	1.32	1.35	10.84
P值	0.82	0.81	0.78	0.02

组别	24 h	48 h	72 h	96 h
空白对照组	0.45±0.03	0.45±0.03	0.45±0.04	0.51±0.04
阴性对照siRNA组	0.49±0.03	0.52±0.04	0.62±0.05	0.61±0.06^a
GLDC siRNA转染组	0.50±0.03	0.48±0.03	0.54±0.04	0.22±0.01^b
F值	1.23	1.32	1.35	10.84
P值	0.82	0.81	0.78	0.02

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