Impact of repeated abortions on growth and reproductive capacity of F1 generation male mice

doi:10.3877/cma.j.issn.1674-0785.2021.07.010

Abstract

Abstract:

Objective

To investigate the impact of repeated abortions on the growth and reproductive capacity of F1 generation male mice.

Methods

F1 generation male mice born by BALB/c mice after two medical abortions induced by intraperitoneal injection of RU486 were used as an experimental group, and F1 generation male mice born by BALB/c mice that had no history of abortions were used as a control group. The survival rate and body weight of F1 generation neonates during the first three weeks after birth were measured. The testicular weight, testicular morphology, epididymis sperm concentration, sperm motility, and reproductive ability of F1 generation male mice were compared between the two groups.

Results

There was no significant difference in neonate survival rate or body weight gain between the two groups (P＞0.05), but the reproductive capacity paramters, including the testicular weight (P＜0.01), sperm concentration (P＜0.05), sperm activity (P＜0.05), and the mating ability (P＜0.05), were significantly lower in the experimental group than in the control group, indicating that the development of the reproductive system and fertility of F1 generation male mice decreased in the experimental group.

Conclusion

The reproductive system development and fertility of F1 male mice can be impaired by repeated abortions of mothers.

Key words: Repeated abortion, Sperm motility, F1 generation, Fertility

Fang Lv, Lili Wang, Xin Wang, Ning Wang, Shucheng Zhang. Impact of repeated abortions on growth and reproductive capacity of F1 generation male mice[J]. Chinese Journal of Clinicians(Electronic Edition), 2021, 15(07): 532-536.

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Figures/Tables 4

组别	雄鼠数（只）	检栓天数（d）	合笼雌鼠（只）	见栓雌鼠（只）	妊娠雌鼠（只）	见栓后妊娠率（%）	着床点数目（ $x ¯$ ±s）	吸收胎率（%， $x ¯$ ±s）	活胎数（只， $x ¯$ ±s）	出生体质量（g， $x ¯$ ±s）
C_G1P1_F1	23	16	75	34	21	61.8	7.0±0.6	15.3±4.8	5.9±0.2	1.41±0.13
T_G3P1_F1	20	16	75	19	13	68.4	6.8±0.8	28.6±9.9	5.0±0.3	1.40±0.22

组别	雄鼠数（只）	检栓天数（d）	合笼雌鼠（只）	见栓雌鼠（只）	妊娠雌鼠（只）	见栓后妊娠率（%）	着床点数目（ $x ¯$ ±s）	吸收胎率（%， $x ¯$ ±s）	活胎数（只， $x ¯$ ±s）	出生体质量（g， $x ¯$ ±s）
C_G1P1_F1	23	16	75	34	21	61.8	7.0±0.6	15.3±4.8	5.9±0.2	1.41±0.13
T_G3P1_F1	20	16	75	19	13	68.4	6.8±0.8	28.6±9.9	5.0±0.3	1.40±0.22

References 21

1	Peixoto P, Cartron PF, Serandour AA, et al. From 1957 to nowadays: a brief history of epigenetics [J]. Int J Mol Sci, 2020, 21(20): 7571.
2	Argyraki M, Damdimopoulou P, Chatzimeletiou K, et al. In-utero stress and mode of conception: impact on regulation of imprinted genes, fetal development and future health [J]. Hum Reprod Update, 2019, 25(6): 777-801.
3	Horton TH. Cross-fostering of voles demonstrates in utero effect of photoperiod [J]. Biol Reprod, 1985, 33(4): 934-939.
4	Meaney MJ, Szyf M, Seckl JR. Epigenetic mechanisms of perinatal programming of hypothalamic-pituitary-adrenal function and health [J]. Trends Mol Med, 2007, 13(7): 269-277.
5	Weaver IC, Cervoni N, Champagne FA, et al. Epigenetic programming by maternal behavior [J]. Nat Neurosci, 2004, 7(8): 847-854.
6	Anway MD, Cupp AS, Uzumcu M, et al. Epigenetic transgenerational actions of endocrine disruptors and male fertility [J]. Science, 2005, 308(5727): 1466-1469.
7	Hales CN, Barker DJ. The thrifty phenotype hypothesis [J]. Br Med Bull, 2001, 60: 5-20.
8	Symonds ME, Sebert SP, Hyatt MA, et al. Nutritional programming of the metabolic syndrome [J]. Nat Rev Endocrinol, 2009, 5(11): 604-610.
9	Correia-Branco A, Keating E, Martel F. Maternal undernutrition and fetal developmental programming of obesity: the glucocorticoid connection [J]. Reprod Sci, 2015, 22(2): 138-145.
10	Vaktskjold A, Tri DV, Odland JO, et al. Parity and birth weight in the Khanh Hoa Province, Vietnam [J]. Open Womens Health J, 2010, 4:1-4.
11	Klemetti R, Gissler M, Niinimäki M, et al. Birth outcomes after induced abortion: a nationwide register-based study of first births in Finland [J]. Hum Reprod, 2012, 27(11): 3315-3320.
12	Brown JSJr, Adera T, Masho SW. Previous abortion and the risk of low birth weight and preterm births [J]. J Epidemiol Community Health, 2008, 62(1): 16-22.
13	王丽丽, 郎楠, 张树成, 等. 重复药物流产小鼠后续妊娠生育结局的研究 [J]. 生殖医学杂志, 2013, 22(9): 697-702.
14	陈忠, 邓卓霖, 李坤雄, 等. 男性不育症睾丸活检诊断的进展 [J]. 广西医科大学学报, 1994, 11(1): 116-119.
15	Petak SM, Nankin HR, Spark RF, et al. American Association of Clinical Endocrinologists Medical Guidelines for clinical practice for the evaluation and treatment of hypogonadism in adult male patients-2002 update [J]. Endocr Pract, 2002, 8(6): 440-456.
16	World Health Organization. Laboratory manual of the WHO for the examination of human semen and sperm-cervical mucus interaction [J]. Ann Ist Super Sanita, 2001, 37(1): 1-123.
17	Sobolewski M, Abston K, Conrad K, et al. Lineage- and sex-dependent behavioral and biochemical transgenerational consequences of developmental exposure to lead, prenatal stress, and combined lead and prenatal stress in mice [J]. Environ Health Perspect, 2020, 128(2): 27001.
18	Hamada H, Matthews SG. Prenatal programming of stress responsiveness and behaviours: progress and perspectives [J]. J Neuroendocrinol, 2019, 31(3): e12674.
19	Cupp AS, Uzumcu M, Suzuki H, et al. Effect of transient embryonic in vivo exposure to the endocrine disruptor methoxychlor on embryonic and postnatal testis development [J]. J Androl, 2003, 24(5): 736-745.
20	Uzumcu M, Suzuki H, Skinner MK. Effect of the anti-androgenic endocrine disruptor vinclozolin on embryonic testis cord formation and postnatal testis development and function [J]. Reprod Toxicol, 2004, 18(6): 765-774.
21	Lv F, Xu XB, Zhang SC, et al. Repeated abortion affects subsequence pregnancy outcomes in BALB/c mice [J]. Plos One, 2012, 7(10): e48384.

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