Emodin enhances succinate dehydrogenase activity and oxidation in Cryptococcus neoformans

doi:10.3877/cma.j.issn.1674-0785.2023.03.014

Abstract

Abstract:

Objective

To explore the effects of emodin on succinate dehydrogenase (SDH) activity and oxidation in Cryptococcus neoformans (C. neoformans) and elaborate the new biological function of emodin, so as to improve the sensitivity of antibacterial drugs targeting SDH and alleviate the serious problem of drug resistance.

Methods

The minimal inhibitory concentration of emodin against C. neoformans was detected by broth microdilution and MTT assay, and the changes of reactive oxygen species (ROS) and mitochondrial membrane potential of C. neoformans treated with emodin were detected. The expression of SDH mRNA was detected by qPCR, and the interaction between emodin and SDH was detected by surface plasmon resonance.

Results

Emodin had no obvious inhibitory effects on C. neoformans, with an MIC＞256 μg/ml, but emodin at a concentration of 1~4 μg/ml was able to promote the SDH activity of C. neoformans. At 1 μg/ml, emodin significantly increased the ROS and SDH mRNA expression. Emodin can bind with SDH specifically.

Conclusion

Emodin can promote the SDH activity and enhance ROS production in C. neoformans, which represents the new biological function of emodin.

Key words: Emodin, Cryptococcus neoformans, Succinate dehydrogenase, Oxidation

Xue Wang, Jiayin Xu, Lijuan Liao, Ganrong Huang, Yongyi Huang, Xian Wei, Yanqiang Huang, Liang Huang. Emodin enhances succinate dehydrogenase activity and oxidation in Cryptococcus neoformans[J]. Chinese Journal of Clinicians(Electronic Edition), 2023, 17(03): 314-319.

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Figures/Tables 8

References 24

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引物名称	序列（5’ to 3’）
GADPH-F	TGAGAAGGACCCTGCCAACA
GADPH-R	ACTCCGGCTTGTAGGCATCAA
CNI03270-F	GCACGAACGATTGCCTGGTA
CNI03270-R	TGAGGACTTCGCCGGTGTAT

引物名称	序列（5’ to 3’）
GADPH-F	TGAGAAGGACCCTGCCAACA
GADPH-R	ACTCCGGCTTGTAGGCATCAA
CNI03270-F	GCACGAACGATTGCCTGGTA
CNI03270-R	TGAGGACTTCGCCGGTGTAT

菌株名称	MIC（μg/ml）
ATCC^® MYA4906	＞256
ATCC^® MYA4907	＞256
BHKS388	＞256
BHKS390	＞256
BHKS480	＞256
BHKS481	＞256
BHKS482	＞256

菌株名称	MIC（μg/ml）
ATCC^® MYA4906	＞256
ATCC^® MYA4907	＞256
BHKS388	＞256
BHKS390	＞256
BHKS480	＞256
BHKS481	＞256
BHKS482	＞256

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