In light of the significant threat posed by Disease X to global public health and the shortcomings of traditional epidemic management models, this study examines precise prevention and control strategies along with implementation pathways for Disease X in large general hospitals. Grounded in risk management and hospital resilience theories, this study focuses on the smallest medical treatment unit as the entry point. It proposes a personalized hierarchical zoning management model for infection prevention and control based on the principle of "one department, one strategy", clarifies the rationale for initiating hierarchical containment, defines the criteria for designating and managing emergency risk zones, specifies operational protocols for infection prevention and control, and formulates strategies for personnel deployment. By developing a "hospital campus-department-ward" tri-zone prevention and control network and a "red-yellow-green" three-tier risk management model, supplemented by visual guidance in the form of "textual specifications + process diagrams", this study achieves precise and differentiated disease prevention and control. The framework offers a practical and viable implementation pathway for the precise prevention and control of Disease X in large general hospitals, providing valuable insights for advancing precise prevention and control of infectious diseases.