Effects of thyroid hormone levels on spatial learning and memory in rats

doi:10.3877/cma.j.issn.1674-0785.2018.06.006

Abstract

Abstract:

Objective

To assess the effect of thyroid hormone levels on spatial memory in Wistar rats.

Methods

Healthy 8-week-old Wistar rats [N=30, approximately (200±20) g, after 1 week of adaptive feeding] were used in this study. The rats were randomly and equally divided into a control group, a hypothyroidism group and a hyperthyroidism group. Normal Wistar rats underwent bilateral thyroidectomy to establish a hypothyroidism model. Hypothyroidism was established 28 days after operation. Normal Wistar rats were injected subcutaneously with thyroxine 20 μg/100 g body weight each day for 28 days to establish a hyperthyroidism model. The Morris water maze test (including navigation and space exploration experiments) was performed on the three groups of rats after the establishment of the models to observe their difference in spatial learning and memory ability.

Results

Compared with control group rats, the escape latency was significantly prolonged in the navigation experiment and the space exploration ability decreased significantly in the hypothyroidism group and hyperthyroidism group, which manifested as statistically significant extended distance and time in the target quadrant and reduced number of passes through the target (P<0.05 for all).

Conclusion

Hypothyroidism or hyperthyroidism can lead to a decrease of spatial memory in Wistar rats.

Key words: Thyroid hormone, Hyperthyroidism, Hypothyroidism, Cognitive function, Spatial memory

Peiren Zhang, Yuntian Yang, Shan Jin, Qiang Zhang, La Ta, Yuntu Wu, Yinbao Bai. Effects of thyroid hormone levels on spatial learning and memory in rats[J]. Chinese Journal of Clinicians(Electronic Edition), 2018, 12(06): 341-346.

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Figures/Tables 9

References 18

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组别	只数	T3（nmoL/L）	T4（nmoL/L）	TSH（uIU/mL）
对照组	10	1.07±0.27^a	3.11±0.78^a	1.37±0.34^a
甲减组	9	0.64±0.07^b	0.78±0.16^b	10.16±3.02^b
甲亢组	10	2.29±1.41	8.09±8.45	0.25±1.17
Z值	?	24.933	20.016	21.449
P值	?	0.0001	0.0001	0.0001

组别	只数	T3（nmoL/L）	T4（nmoL/L）	TSH（uIU/mL）
对照组	10	1.07±0.27^a	3.11±0.78^a	1.37±0.34^a
甲减组	9	0.64±0.07^b	0.78±0.16^b	10.16±3.02^b
甲亢组	10	2.29±1.41	8.09±8.45	0.25±1.17
Z值	?	24.933	20.016	21.449
P值	?	0.0001	0.0001	0.0001

分组	目标象限时间（s）	目标象限路程（cm）	平均速度（cm/s）	穿越目标象限次数（次）50%（25%，75%）	总路程（cm）
对照组	58.23±21.69	499.83±155.80	14.27±2.24	4.0（3.0，5.0）	1711.8±268.87
甲亢组	29.97±5.35	279.81±99.98	17.27±1.81	1.5（1.3，1.06）	1772.3±250.71
甲减组	19.04±6.17	153.81±90.52	11.14±2.78	1.0（1.0，1.0）	1656.2±2393.6
统计值	F′=18.571	F′=17.546	F=16.957	Z=17.191	F=0.339
P值	<0.0001	<0.0001	<0.0001	<0.001	0.715

分组	目标象限时间（s）	目标象限路程（cm）	平均速度（cm/s）	穿越目标象限次数（次）50%（25%，75%）	总路程（cm）
对照组	58.23±21.69	499.83±155.80	14.27±2.24	4.0（3.0，5.0）	1711.8±268.87
甲亢组	29.97±5.35	279.81±99.98	17.27±1.81	1.5（1.3，1.06）	1772.3±250.71
甲减组	19.04±6.17	153.81±90.52	11.14±2.78	1.0（1.0，1.0）	1656.2±2393.6
统计值	F′=18.571	F′=17.546	F=16.957	Z=17.191	F=0.339
P值	<0.0001	<0.0001	<0.0001	<0.001	0.715

时间	对照组	甲亢组	甲减组
第1天	48.69±33.70	83.31±42.29	91.50±36.76
第2天	28.43±26.09^a	59.47±47.04^a	66.82±44.66^a
第3天	21.48±12.97^b	68.99±38.71	66.73±35.29^b
第4天	19.23±17.58^c	46.00±35.99^cd	55.64±35.69^c

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