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Chinese Journal of Clinicians(Electronic Edition) ›› 2020, Vol. 14 ›› Issue (11): 917-921. doi: 10.3877/cma.j.issn.1674-0785.2020.11.013

Special Issue:

• Basic Science Research • Previous Articles     Next Articles

Effect of oxymatrine on ventricular arrhythmia induced by acute myocardial infarction in rats

Guowei Qin1, Suining Li2, Tao Zhou2,()   

  1. 1. Department of Electrocardiographic Diagnosis, People's Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
    2. Department of Cardiac Surgery, Guizhou Provincial People's Hospital, Guiyang 550002, China.
  • Received:2020-04-21 Online:2020-11-15 Published:2021-03-23
  • Contact: Tao Zhou

Abstract:

Objective

To investigate the effect of oxymatrine (OMT) on ventricular arrhythmia (VA) induced by myocardial infarction (MI) in rats and explore the possible mechanism.

Methods

Seventy-five healthy male SD rats were randomly divided into a sham operation group, an MI group, and an OMT intervention group, with 25 rats in each group. MI was induced by ligation of the left anterior descending coronary artery in the MI group and OMT group. OMT (100 mg/kg) was administered in the OMT group at two weeks before and two weeks after the establishment of the model. At the end of drug intervention, echocardiography was used to evaluate left ventricular function. The levels of NT-proBNP and hs-TnT in plasma were detected by ELISA. Cx43 mRNA and protein expression in the myocardium weas detected of by RT-PCR and Western blot, respectively. VA was induced by burst stimulation.

Results

The levels of NT-proBNP and hs-TnT in the OMT group were significantly lower than those in the MI group [(256.45±38.55) ng/L vs (371.93±41.23)ng/L, (89.55±13.39)ng/L vs (132.63±20.12)ng/L; P<0.01]. The relative expression of Cx43 mRNA in the sham, MI, and OMT group was 0.93±0.16, 0.47±0.06 and 0.71±0.12 respectively, the relative expression of Cx43 protein in the three groups was 0.77±0.11, 0.50±0.07 and 0.61±0.08, respectively, and the relative expression of p-Cx43 protein was 0.62±0.09, 0.32±0.05 and 0.50±0.07 respectively; the differences among the three groups were statistically significant (P<0.05 for all). In addition, the VA induction rate in the OMT group was significantly lower than that of the MI group (21.4% vs 64.3%, P<0.05).

Conclusion

OMT can reduce the incidence of myocardial injury and VA in MI rats, and the underlying mechanism may be related to the regulation of Cx43 expression by OMT.

Key words: Oxymatrine, Myocardial infarction, Arrhythmia, Gap junction protein 43

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