Significance of GSDMD-dependent pyroptosis in patients with snakebite

doi:10.3877/cma.j.issn.1674-0785.2021.03.006

Abstract

Abstract:

Objective

To investigate the clinical significance of GSDMD-dependent pyroptosis in patients with poisonous snake bites.

Methods

Patients with poisonous snake bites who were hospitalized at Department of Emergency Medicine, the First Hospital of Guangxi Medical University from June 2018 to August 2019 were selected as a study group, and healthy volunteers during the same period were selected as a control group. The levels of serum GSDMD, IL-1β, and IL-18 in the study group and control group were measured by enzyme-linked immunosorbent assay (ELISA).

Results

The contents of GSDMD [13.662 (12.608, 17.663) pg/ml], IL-1β [14.262 (12.022, 16.442) pg/ml], and IL-18 [36.104 (31.974, 53.927) pg/ml] in the serum of poisoning patients with cobra bites were significantly higher than those in the control group [GSDMD: 9.260 (9.112, 9.720) pg/ml; IL-1β: 12.597 (6.250, 13.808) pg/ml; IL-18: 31.783 (24.080, 35.208) pg/ml] (P＜0.05), but there was no significant difference in GSDMD, IL-1β, IL-18 between patients with trimeresurus stejnegeri bites [GSDMD: 9.472 (9.318, 10.705) pg/ml; IL-1β: (12.005±5.377) pg/ml; IL-18: (35.227±19.643) pg/ml] and the control group [GSDMD 9.260 (9.112，9.720) pg/ml;IL-1β (11.017±3.852) pg/ml;IL-18 (29.933±5.476) pg/ml] (P＞0.05).

Conclusion

GSDMD, IL-1,β and IL-18 have increased expression in patients with cobra bite poisoning, which may be involved in the immune inflammatory reaction.

Key words: Pyroptosis, Snakebite, Gasdermin D, Interleukin-1β, Interleukin-18

Zeyu Yang, Xia Yang, Jiawei Wang, Xinling Tan, Minmin Zhang, Zong Ning. Significance of GSDMD-dependent pyroptosis in patients with snakebite[J]. Chinese Journal of Clinicians(Electronic Edition), 2021, 15(03): 187-190.

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URL: https://zhlcyszz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0785.2021.03.006

https://zhlcyszz.cma-cmc.com.cn/EN/Y2021/V15/I03/187

Figures/Tables 2

组别	例数	GSDMD	IL-1β	IL-18
眼镜蛇咬伤组	12	13.662（12.608，17.663）	14.262（12.022，16.442）	36.104（31.974，53.927）
对照组	10	9.260（9.112，9.720）	12.597（6.250，13.808）	31.783（24.080，35.208）
Z值		-3.890	-1.978	-2.242
P值		0.000	0.048	0.025

组别	例数	GSDMD［M（Q₂₅，Q₇₅）］	IL-1β（ $x ¯$ ±s）	IL-18（ $x ¯$ ±s）
竹叶青咬伤组	9	9.472（9.318，10.705）	12.005±5.377	35.227±19.643
对照组	10	9.260（9.112，9.720）	11.017±3.852	29.933±5.476
统计值		Z=-1.470	t=-0.464	t=-0.820
P值		0.142	0.649	0.424

组别	例数	GSDMD［M（Q₂₅，Q₇₅）］	IL-1β（ $x ¯$ ±s）	IL-18（ $x ¯$ ±s）
竹叶青咬伤组	9	9.472（9.318，10.705）	12.005±5.377	35.227±19.643
对照组	10	9.260（9.112，9.720）	11.017±3.852	29.933±5.476
统计值		Z=-1.470	t=-0.464	t=-0.820
P值		0.142	0.649	0.424

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