Association between NLRP3 gene polymorphisms and inflammatory marks in elderly patients with acute myocardial infarction

doi:10.3877/cma.j.issn.1674-0785.2022.03.009

Abstract

Abstract:

Objective

To investigate the association between NLRP3 gene polymorphisms and inflammatory marks in elderly patients with acute myocardial infarction.

Methods

One hundred and twenty-six elderly patients with acute myocardial infarction at the Second Affiliated Hospital of Bengbu Medical College from January 2019 to December 2021 were selected as a study group, and 100 healthy people were selected as a control group. Fasting venous blood was collected from all subjects to detect serum high-sensitivity C-reactive protein (hs-CRP), interleukin-1β (IL-1β), interleukin-18 (IL-18), and NLRP3 levels, and the differences between the study group and control group were compared. The polymorphisms of the NLPR3 gene (rs10754558 and rs35829419) were detected in the study group, and the differences in serum hs-CRP, IL-1β, and IL-18 levels in patients with different genotypes were compared.

Results

The levels of hs-CRP, IL-1β, IL-18, and NLRP3 in the study group were significantly higher than those in the control group [(4.8±2.3) mg/dl vs (1.4±0.9) mg/dl, P＜0.001; (186.4±18.3) ng/ml vs (46.3±16.7) ng/ml, P＜0.001; (241.6±32.5) pg/ml vs (124.6±28.1) pg/ml, P＜0.001; (1.94±0.65) pg/ml vs (0.92±0.54) pg/ml, P＜0.001], and the positive rate of troponin was significantly higher than that of the control group (72.22% vs 0, P＜0.001). In the study group, the GG genotype of rs10754558 was associated with the highest levels of serum hs-CRP [(5.6±2.4) mg/L], IL-1β [(217.6±16.7) ng/ml], and IL-18 [(264.3±24.7) pg/ml], followed by the GC genotype [(4.6±2.1) mg/L, (177.1±16.3) ng/ml, and (236.8±24.1) pg/ml, respectively] and the CC genotype [(4.0±2.1) mg/L, (156.4±15.9) ng/ml, and (210.2±23.9) pg/ml, respectively]; the differences between any two of them were statistically significant (P＜0.05). The AA genotype of rs35829419 was associated with the highest levels of hs-CRP [(7.0±1.9) mg/L], IL-1β [(229.2±17.2) ng/ml], and IL-18 [(285.3±28.6) pg/ml], followed by the AC genotype [(5.3±2.0) mg/L, (194.3±16.8) ng/ml, and (253.4±28.9) pg/ml, respectively] and the CC genotype [(4.6±1.8) mg/L, (183.0±17.0) ng/ml, and (236.3±28.2) pg/ml, respectively]; the differences between any two of them were statistically significant (P＜0.05). The GG+GC [odds ratio [OR]=1.726, 95%CI 1.306-4.018, P＜0.001] and AA+AC [OR=4.617, 95%CI 2.512-8.019, P＜0.001] genotypes were independent risk factors for acute myocardial infarction.

Conclusion

The NLRP3 gene polymorphisms rs10754558 and rs35829419 affect serum inflammatory marks levels in elderly patients with acute myocardial infarction.

Key words: Myocardial infarction, High-sensitivity C-reactive protein, Interleukin-1β, Interleukin-18, Gene polymorphism, Nod-like receptor protein 3

Wenjun Wu, Sha Sha, Cong Tian, Jian Zhu. Association between NLRP3 gene polymorphisms and inflammatory marks in elderly patients with acute myocardial infarction[J]. Chinese Journal of Clinicians(Electronic Edition), 2022, 16(03): 241-245.

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URL: https://zhlcyszz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0785.2022.03.009

https://zhlcyszz.cma-cmc.com.cn/EN/Y2022/V16/I03/241

Figures/Tables 5

项目	研究组（126例）	对照组（100例）	统计值	P值
年龄（岁， $x ¯$ ±s）	66.7±5.9	65.9±5.3	t=1.059	0.291
男性［例（%）］	67（53.17）	57（57.00）	χ²=0.329	0.566
收缩压（mmHg， $x ¯$ ±s）	128.6±10.2	120.2±9.4	t=6.365	＜0.001
舒张压（mmHg， $x ¯$ ±s）	89.3±7.6	83.1±6.9	t=6.343	＜0.001
hs-CRP（mg/dl， $x ¯$ ±s）	4.8±2.3	1.4±0.9	t=15.193	＜0.001
肌钙蛋白阳性［例（%）］	91（72.22）	0（0）	χ²=120.905	＜0.001
NLRP3（ng/ml， $x ¯$ ±s）	1.94±0.65	0.92±0.54	t=12.612	＜0.001
IL-1β（ng/ml， $x ¯$ ±s）	186.4±18.3	46.3±16.7	t=59.401	＜0.001
IL-18（pg/ml， $x ¯$ ±s）	241.6±32.5	124.6±28.1	t=28.518	＜0.001

项目	研究组（126例）	对照组（100例）	统计值	P值
年龄（岁， $x ¯$ ±s）	66.7±5.9	65.9±5.3	t=1.059	0.291
男性［例（%）］	67（53.17）	57（57.00）	χ²=0.329	0.566
收缩压（mmHg， $x ¯$ ±s）	128.6±10.2	120.2±9.4	t=6.365	＜0.001
舒张压（mmHg， $x ¯$ ±s）	89.3±7.6	83.1±6.9	t=6.343	＜0.001
hs-CRP（mg/dl， $x ¯$ ±s）	4.8±2.3	1.4±0.9	t=15.193	＜0.001
肌钙蛋白阳性［例（%）］	91（72.22）	0（0）	χ²=120.905	＜0.001
NLRP3（ng/ml， $x ¯$ ±s）	1.94±0.65	0.92±0.54	t=12.612	＜0.001
IL-1β（ng/ml， $x ¯$ ±s）	186.4±18.3	46.3±16.7	t=59.401	＜0.001
IL-18（pg/ml， $x ¯$ ±s）	241.6±32.5	124.6±28.1	t=28.518	＜0.001

基因	研究组（126例）	对照组（100例）	χ²值	P值
rs10754558				7.656	0.022
基因型
	CC	27（21.43）	38（38.00）
	GG	45（35.71）	26（26.00）
	GC	54（42.86）	36（36.00）
基因频率				7.710	0.005
	C	108（42.86）	112（56.00）
	G	144（57.14）	88（44.00）
rs35829419				19.939	＜0.001
基因型
	AA	6（4.76）	0（0）
	CC	98（77.78）	98（98.00）
	AC	22（17.46）	2（2.00）
基因频率				23.739	＜0.001
	A	34（13.49）	2（1.00）
	C	218（86.51）	198（99.00）

基因型	例数	hs-CRP（mg/L， $x ¯$ ±s）	肌钙蛋白阳性［例（%）］	NLRP3（ng/ml， $x ¯$ ±s）	IL-1β（ng/ml， $x ¯$ ±s）	IL-18（pg/ml， $x ¯$ ±s）
CC	27	4.0±2.1	15（55.56）	1.2±0.7	156.4±15.9	210.2±23.9
GC	54	4.6±2.1^a	38（70.37）^a	1.8±0.6^a	177.1±16.3^a	236.8±24.1^a
GG	45	5.6±2.4^ab	38（84.44）^ab	2.4±0.6^ab	217.6±16.7^ab	264.3±24.7^ab
统计值		F=4.920	χ²=7.182	F=32.250	F=135.810	F=43.270
P值		0.009	0.028	＜0.001	＜0.001	＜0.001

基因型	例数	hs-CRP（mg/L， $x ¯$ ±s）	肌钙蛋白阳性［例（%）］	NLRP3（ng/ml， $x ¯$ ±s）	IL-1β（ng/ml， $x ¯$ ±s）	IL-18（pg/ml， $x ¯$ ±s）
CC	27	4.0±2.1	15（55.56）	1.2±0.7	156.4±15.9	210.2±23.9
GC	54	4.6±2.1^a	38（70.37）^a	1.8±0.6^a	177.1±16.3^a	236.8±24.1^a
GG	45	5.6±2.4^ab	38（84.44）^ab	2.4±0.6^ab	217.6±16.7^ab	264.3±24.7^ab
统计值		F=4.920	χ²=7.182	F=32.250	F=135.810	F=43.270
P值		0.009	0.028	＜0.001	＜0.001	＜0.001

基因型	例数	hs-CRP（mg/L， $x ¯$ ±s）	肌钙蛋白阳性［例（%）］	NLRP3（ng/ml， $x ¯$ ±s）	IL-1β（ng/ml， $x ¯$ ±s）	IL-18（pg/mL， $x ¯$ ±s）
CC	98	4.6±1.8	65（66.33）	1.9±0.7	183.0±17.0	236.3±28.2
AC	22	5.3±2.0^a	20（90.91）^a	2.3±0.6^a	194.3±16.8^a	253.4±28.9^a
AA	6	7.0±1.9^ab	6（100.00）^ab	3.0±0.6^ab	229.2±17.2^ab	285.3±28.6^ab
统计值		F=5.660	χ²=7.835	F=9.620	F=23.290	F=10.760
P值		0.005	0.020	＜0.001	＜0.001	＜0.001

基因型	例数	hs-CRP（mg/L， $x ¯$ ±s）	肌钙蛋白阳性［例（%）］	NLRP3（ng/ml， $x ¯$ ±s）	IL-1β（ng/ml， $x ¯$ ±s）	IL-18（pg/mL， $x ¯$ ±s）
CC	98	4.6±1.8	65（66.33）	1.9±0.7	183.0±17.0	236.3±28.2
AC	22	5.3±2.0^a	20（90.91）^a	2.3±0.6^a	194.3±16.8^a	253.4±28.9^a
AA	6	7.0±1.9^ab	6（100.00）^ab	3.0±0.6^ab	229.2±17.2^ab	285.3±28.6^ab
统计值		F=5.660	χ²=7.835	F=9.620	F=23.290	F=10.760
P值		0.005	0.020	＜0.001	＜0.001	＜0.001

项目	B值	SE	Wald χ²值	OR（95%CI）	P值
rs10754558
CC （参照）	-	-	-	1.000	-
GG+GC	1.612	0.618	9.617	1.726（1.306~4.018）	＜0.001
rs35829419
CC （参照）	-	-	-	1.000	-
AA+AC	0.961	0.219	18.506	4.617（2.512~8.019）	＜0.001

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