Objective To explore the effect of the mode of the sense control supervisor on the prevention and control of nosocomial infection and the ability of nurses to prevent and control. Methods From March 2019 to February 2021, the inpatients and on duty nurses in the Second Affiliated Hospital of Zhengzhou University were observed. According to the implementation time of the feeling control supervisor model, they were divided into two groups: pre intervention group and post intervention group. The incidence of hospital infection, the qualified rate of environmental monitoring, the compliance rate of hand hygiene, the incidence of occupational exposure and the ability of nursing staff to prevent and control hospital infection in the two groups were statistically analyzed. Results The incidence of nosocomial infection after intervention was 0.37%, which was significantly lower than that before intervention (0.54%) (2=7.047, P=0.008); The qualified rate of environmental sample monitoring before intervention was 83.82%, and the compliance rate of doctors and nurses was 63.43%, which was significantly lower than 92.06% and 72.09% after intervention (P＜0.05); The incidence of occupational exposure after intervention was significantly lower than that before intervention (2.22% vs 4.38%, P＜0.05); The nursing staff's hospital sense prevention and control ability was evaluated with the Gerson Hospital Safety Atmosphere Scale (C-HSCS). It was found that the nursing staff's hospital sense prevention and control ability was significantly improved after the intervention (P＜0.05). Conclusion The implementation of the feeling control supervisor model can effectively reduce the incidence of hospital infection, optimize the hospital environment, reduce occupational exposure, improve the compliance of hand hygiene and the ability of nurses to prevent and control hospital infection, which can be popularized in clinical practice.