Progress in research of detection and detoxification methods for aflatoxin B1 in Semen platyclade

doi:10.3877/cma.j.issn.1674-0785.2023.12.004

Abstract

Abstract:

Aspergillus flavus is easy to grow and produce toxin in the process of harvesting, drying, peeling, wind sorting, transportation, and storage of Semen platycladi. This not only seriously affects the quality of Semen platycladi, but also causes carcinogenic, teratogenic, and mutagenic effects. Therefore, there is an urgent need to solve the problem of Aspergillus flavus pollution. In order to strengthen the standardized storage management of oily traditional Chinese medicines such as Semen platycladi, this paper reviews the detection and detoxification methods for aflatoxin B1, and proposes that the application of immunosensors has the advantages of fast detection, high sensitivity, high specificity, and automation. The biological detoxification method is more efficient and environmentally friendly, and the degradation of the fermentation broth without toxins can effectively remove the accumulated mycotoxins. Natural plant volatile oils also have an inhibitory effect on the growth of Aspergillus flavus and the synthesis of aflatoxin B₁.

Key words: Semen Platycladi, Aspergillus flavus, Aflatoxin B₁, Detection, Detoxification

Ying Li, Yingying Cheng, Qijin Shi, Chen Gao, Hui Yang, Min Jin. Progress in research of detection and detoxification methods for aflatoxin B_{₁ in Semen platyclade[J]. Chinese Journal of Clinicians(Electronic Edition), 2023, 17(12): 1223-1227.}

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References 54

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基质	提取	检测	检出限	文献
花生油	丙酮-水	薄层色谱法	–	[6]
沙棘	70%甲醇	超高效液相色谱-串联质谱法	–	[7]
蜂房	70%甲醇	柱后光衍生-高效液相色谱法	0.42 μg/kg	[8]
饲料	乙腈/水	高效液相色谱法	–	[10]
饲料	70%甲醇	酶联免疫吸附法	–	[10]
玉米	甲醇-水	电化学生物传感器	0.47 ng/L	[12]
花生	甲醇-水	电化学发光免疫分析	5.00 ng/L	[13]
谷物	甲醇：水	免疫聚合酶链反应	0.03 μg/L	[15]
玉米	甲醇-水	电化学发光传感器	3.50 fg/ml	[18]
玉米油	甲醇	电化学发光传感器	8.50 fg/ml	[19]
粮食	甲醇-水	拉曼光谱仪–胶体金传感器	0.50 μg/L	[20]
食品	甲醇-水	循环微流控芯片	0.06 μg/L	[21]
小米	70%甲醇	电化学适体传感器	6.30 ng/L	[24]

基质	提取	检测	检出限	文献
花生油	丙酮-水	薄层色谱法	–	[6]
沙棘	70%甲醇	超高效液相色谱-串联质谱法	–	[7]
蜂房	70%甲醇	柱后光衍生-高效液相色谱法	0.42 μg/kg	[8]
饲料	乙腈/水	高效液相色谱法	–	[10]
饲料	70%甲醇	酶联免疫吸附法	–	[10]
玉米	甲醇-水	电化学生物传感器	0.47 ng/L	[12]
花生	甲醇-水	电化学发光免疫分析	5.00 ng/L	[13]
谷物	甲醇：水	免疫聚合酶链反应	0.03 μg/L	[15]
玉米	甲醇-水	电化学发光传感器	3.50 fg/ml	[18]
玉米油	甲醇	电化学发光传感器	8.50 fg/ml	[19]
粮食	甲醇-水	拉曼光谱仪–胶体金传感器	0.50 μg/L	[20]
食品	甲醇-水	循环微流控芯片	0.06 μg/L	[21]
小米	70%甲醇	电化学适体传感器	6.30 ng/L	[24]

对象	处理方法	抑制率(%)	原理	文献
柏子仁	75%乙醇(1∶2)，搅拌5 min，过滤，80 ℃干燥3~4 h	90.5	溶剂萃洗(物理法)	[37]
柏子仁	室温下用6~8 kGy的γ射线辐照后予以乙醇、水洗2~3次，烘干	>50.5	辐射法(物理法)	[38]
花生油	0.17%的组氨酸改性蒙脱土在110 ℃下吸附30 min	98.2	吸附法(物理法)	[39]
花生油	10%的碱液处理、30 min	90.7	碱液处理法(化学法)	[42]
花生油	7.5%的碱液处理、25 min	98.0	碱液处理法(化学法)	[43]
花生油	1.5 mg/g的磁性多壁碳纳米管吸附60 min	92.36	吸附法(物理法)	[44]
柏子仁	用嗜麦芽窄食单胞菌的上清液、发酵液和胞外蛋白粗提取液在37 ℃脱除72 h	70.5~76.0	降解作用(生物法)	[49]
花生粕	白芝发酵料水比1∶2、30 ℃降解12 h	90.7	生物降解(生物法)	[51]
黄曲霉	贝莱斯芽孢杆菌胞外酶处理72 h	88.24	降解作用(生物法)	[52]
黄曲霉	丁酸梭菌发酵液酶蛋白处理72 h	68.65	降解作用(生物法)	[53]

对象	处理方法	抑制率(%)	原理	文献
柏子仁	75%乙醇(1∶2)，搅拌5 min，过滤，80 ℃干燥3~4 h	90.5	溶剂萃洗(物理法)	[37]
柏子仁	室温下用6~8 kGy的γ射线辐照后予以乙醇、水洗2~3次，烘干	>50.5	辐射法(物理法)	[38]
花生油	0.17%的组氨酸改性蒙脱土在110 ℃下吸附30 min	98.2	吸附法(物理法)	[39]
花生油	10%的碱液处理、30 min	90.7	碱液处理法(化学法)	[42]
花生油	7.5%的碱液处理、25 min	98.0	碱液处理法(化学法)	[43]
花生油	1.5 mg/g的磁性多壁碳纳米管吸附60 min	92.36	吸附法(物理法)	[44]
柏子仁	用嗜麦芽窄食单胞菌的上清液、发酵液和胞外蛋白粗提取液在37 ℃脱除72 h	70.5~76.0	降解作用(生物法)	[49]
花生粕	白芝发酵料水比1∶2、30 ℃降解12 h	90.7	生物降解(生物法)	[51]
黄曲霉	贝莱斯芽孢杆菌胞外酶处理72 h	88.24	降解作用(生物法)	[52]
黄曲霉	丁酸梭菌发酵液酶蛋白处理72 h	68.65	降解作用(生物法)	[53]

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